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An important aspect of the iGEM competition is the use and creation of standard biological parts.
Here is a table of summary of the parts that we constructed or have submitted to the registry
Name Natural function of part Usage Copies BBa_K1648000 Basic Part flanking sequence of mamHIEJKLMN from Magnetosome Island (MAI) Template for homologous recombination with mamAB operon In pSB1C3 (submitted) BBa_K1648001 Basic Part flanking sequence of mamOPAQRBSTU from Magnetosome Island (MAI) Template for homologous recombination with mamGC, mamXY, mms operons (not submitted) BBa_K1648002 Composite part Biobrick for homologous recombination with mamAB operon from Magnetosome Island (MAI) Template for homologous recombination with mamAB operon(contains a promotor and terminator) In pSB1A3 (stocked) In pSB1C3 (submitted) BBa_K1648003 Composite part Biobrick for homologous recombination with mamGC, mamXY, mms operons from Magnetosome Island (MAI) Template for homologous recombination with mamGC, mamXY, mms operons(contains a promotor and terminator) In pSB1C3 (submitted) BBa_K1648004 Basic Part Biobrick for mamC fuse protein on magnetosome membrane Template for fuse protein of interest to magnetosome membrane In pSB1C3 (submitted) BBa_K1648005 Basic Part Functional biobrick for GFP nanobody Express GFP nanobody to conduct immunoprecipitation with GFP In pSB1C3 (submitted) BBa_K1648006 Composite part Functional biobrick for mamC fuse protein on magnetosome membrane and GFP nanobody fuse GFP nanobody to magnetosome membrane In pSB1C3 (submitted) BBa_K1648045 oprF from Azotobacter vinelandii Allows non-specific transport from bacteria and extracellular environment In pSB1C3 (submitted) BBa_K1648046 Mutated oprF from Azotobacter vinelandii with five-point mutations Give 5-fold transmission efficiency than original oprF Planing BBa_K1648047 oprF from Azotobacter vinelandii with RBS Intermediate part for transcription of oprF Planing BBa_K1648048 oprF from Azotobacter vinelandii promoted by tetRpro Constitutive production of oprF which allows non-specific transport from bacteria and extracellular environment In pSB1A3 (submitted) BBa_K1648049 Mutated oprF from Azotobacter vinelandii with five-point mutations promoted by tetRpro Constitutive production of mutated oprF In pSB1C3 (submitted) BBa_K1648050 suspecting porin from Pseudomonas fluorescens with constitutive promoter Expression of oprF for characterization In pSB1C3 (submitted) BBa_K1648051 Forming porin on Azotobacter vinelandii and templates for overexpressing hydrogenase in Azotobacter vinelandii overexpress both porin and hydrogenase once transformed into Azotobacter vinelandii with homologous recombination In pSB1C3 (submitted)
