Team:Michigan/Notebook/September

September

Work plan:

LacZ into Thrombin plasmid:

• cloning not working probably because the insert is almost as big as backbone let’s transform LacZ again
• transform in vitro LacZ part again
• plasmid is ready, digest with HindiIII and A
• gel extract plasmid, measure dna concentration

LacZ into Gliadin plasmid:
Pfldh plasmid with GFP:
measure fluorescence

Things to order:

• aptamer and trigger for Gliadin
• switch for Gliadin with GFP (check design folder)
• PfLDH protein
• aptamer and trigger for Pfldh
• aptamer and trigger for Kanamycin