Metodology

The methodology consists of cutting and pasting, with ligases and restriction enzymes as a "waterfall", for final assembly that will produce both proteins. The motive of this assembly is due to the fact that the promoter, the RBS and the terminator were too small, and if you take out them from their original plasmids, these are lost, so, simply sticking and peeling it off one by one, we arrived to a linear Assembly in the three corresponding plasmids (promoter, RBS and terminator).

At this time we are innovating ideas to add a circuit that will allow us in the future to obtain a method of detecting and quantifying the presence of gluten, which can also be checked by a commercial kit.

Team:San Andres/Modeling

Metodology