Team:UCLA/Parts

iGEM UCLA




PARTS

Honeybee Parts

  1. [http://parts.igem.org/Part:BBa_K1763000 BBa_K1763000 ] This part is the coding region of honeybee silk protein #3. For the sequence click here . Click on the link for more details on how the sequence was designed and chosen, as well as how the cloning was carried out.
  2. [http://parts.igem.org/Part:BBa_K1763001 BBa_K1763001] This part is a composite part of the honeybee silk protein #3 coding region along with [http://parts.igem.org/Part:BBa_R0010 BBa_R0010 Promoter ] and [http://parts.igem.org/Part:BBa_B0034 RBS BBa_B0034 ]. For the sequence click here . Click HERE for more details on how the sequence was designed and chosen, as well as how the cloning was carried out.
  3. [http://parts.igem.org/Part:BBa_K1763008 BBa_K1763008]. This sequence is a fusion protein between our honeybee silk and the Spycatcher, protein which has specific affinity for SpyCatcher. It also contains regulatory elements like a promoter and rbs for expression.
  4. [http://parts.igem.org/Part:BBa_K1763007 BBa_K1763007] is our honeybee silk sequence with a T7 promoter upstream for effective protein expression in bacterial strains containing t7 polymerase.

Spider Silk Parts

  1. [http://parts.igem.org/Part:BBa_K1763002 BBa_K1763002]: Major Ampullate Spidroin 2, construct AB. Used for Iterative Capped Assembly of Spider Silk Genes.
  2. [http://parts.igem.org/Part:BBa_K1763003 BBa_K1763003]: Major Ampullate Spidroin 2, construct BC. Used for Iterative Capped Assembly of Spider Silk Genes.
  3. [http://parts.igem.org/Part:BBa_K1763003 BBa_K1763004]: Major Ampullate Spidroin 2, construct CA. Used for Iterative Capped Assembly of Spider Silk Genes.

Protein Cage Parts

Silk Functionalization Parts