Team:UCLA/Notebook/Spider Silk Genetics/22 July 2015
7/22/2015
Transformation Results
- Colonies are present on all plates from yesterday.
Miniprep
- Used Zymo Classic kit to prep M2-9(T7) and M2-SeqAB
Concentration (ng/uL) | A 260/280 | |
---|---|---|
M2-9(T7)-1 | 590.49 | 1.86 |
M2-9(T7)-2 | 614.07 | 1.84 |
M2-9(T7)-3 | 392.19 | 1.84 |
M2-Seq AB | 1117.69 | 1.88 |
BsaI Digestion for M2-Seq AB
- Digest 5 ug of M2-SeqAB plasmid in 50 uL reaction with 4 uL of BsaI.
Volume (uL) | |
---|---|
BsaI | 4 |
10x Cutsmart | 5 |
DNA | 4.47 |
ddH2O | 36.53 |
- Ran digest on 1.5% TAE gel. Used 2 uL of 50 bp ladder.
- The digestion worked, but it is not as complete as previous digestions have been. Compare to Digestion of MaSp2 Plasmids and Gel Purification previously.