Difference between revisions of "Team:Aachen/Lab/Bioreactor"

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{{Team:Aachen/Header}}
 
{{Team:Aachen/Header}}
 
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= frist heading =
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{{Team:Aachen/ReadMore|title=Download Library|link=/Team:Aachen/Lab/Bioreactor/Download_Library|picture=rmDownloadLibrary}}
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{{Team:Aachen/ReadMore|title=<html><span style="font-size:18px">Characterization of Bioreactor</span></html>|link=/Team:Aachen/Lab/Bioreactor/Characterization_of_Bioreactor|picture=rmReactorCharacterization|url=/wiki/images/6/64/Aachen_Reactor_Cracterisation_Wide.JPG}}
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{{Team:Aachen/ReadMore|title=Control Software|link=/Team:Aachen/Software|picture=rmSoftware|url=/wiki/images/7/7a/Aachen_tile_Lab_Bioreactor_controlsoftware.JPG}}
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{{Team:Aachen/ReadMore|title=Hardware|link=/Team:Aachen/Lab/Bioreactor/Hardware|picture=rmHardware|url=/wiki/images/d/d9/Aachen_BioreactorAssembledPump1.jpg}}
 
As our cloning tools get more and more advanced, the next bottleneck starts to show up: strain characterization.
 
As our cloning tools get more and more advanced, the next bottleneck starts to show up: strain characterization.
  
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For metabolic engineering the chemostat cultivation is one of the most important characterization experiments. But not only are 200-1000&nbsp;ml chemostats expensive to run, they are also not easy to standardize.
  
For metabolic engineering the chemostat cultivation is one of the most important characterization experiments. But not only are 200-1000&nbsp;ml chemostats expensive to run, they are also not parallelizable well.
 
  
 
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To increase the effectiveness of metabolic engineering research, we made a Do-It-Yourself chemostat bioreactor in the 10&nbsp;ml scale.
To increase the effectiveness of metabolic engineering research, we made a DIY chemostat bioreactor in the 10&nbsp;ml scale.
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In addition to that we want to do our bit in spreading biotechnology.
 
In addition to that we want to do our bit in spreading biotechnology.
At the moment professional lab equipment is not affordable for smaller institutions like schools, community labs and even small universities. If synthetic biology should be announced to a wider public, it is necessary that education can start way earlier. Therefore our reactor system is cheap and DIY, that even school classes can assemble and use them in their biology courses and start exploring the world of biotechnology.
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At the moment professional lab equipment is not affordable for smaller institutions like schools, community labs and even small universities. If synthetic biology should be propagated in a wider public, it is necessary that its education starts early. Therefore our reactor system is DIY and inexpensive, so that it can be assembled in schools.
 
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{{Team:Aachen/DoubleFigure|Aachen_Reactor_Overview_1.JPG|Aachen_Reactor_Overview_3.JPG|title1=Tubes coming out of the reactors|title2=OD device and pump box|size=medium}}
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We focused on building a highly modular system. Requirements on lab equipment change from experiment to experiment. Therefore it is useless to create a closed system that can do only one task at all. It should not matter if you need six pumps or just three analysis units or three whole reactors. With our system that is all possible and even more: The open implementation of the MCP makes it possible to easily add new components and stay ready for future requirements.
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{{Team:Aachen/ReadMore|title=Download Library|link=/Team:Aachen/Lab/Bioreactor/Download_Library|picture=rmDownloadLibrary}}
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{{Team:Aachen/ReadMore|title=Hardware|link=/Team:Aachen/Lab/Bioreactor/Hardware|picture=rmHardware}}
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{{Team:Aachen/ReadMore|title=Control Software|link=/Team:Aachen/Software|picture=rmSoftware|url=/wiki/images/7/7a/Aachen_tile_Lab_Bioreactor_controlsoftware.JPG}}
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{{Team:Aachen/ReadMore|title=<html><span style="font-size:14px">Characterization of Bioreactor</span></html>|link=/Team:Aachen/Lab/Bioreactor/Characterization_of_Bioreactor|picture=rmDownloadLibrary|url=/wiki/images/2/20/Aachen_DownloadLibrary.jpg}}
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{{Team:Aachen/DoubleFigure|Aachen_Reactor_Overview_4.JPG|Aachen_Reactor_Overview_3.JPG|title1=Overview over the whole system|title2=OD device and pump box|subtitle1=The reactors with all tubes connected can be autoclaved while assembled and set up staying sterile afterwards|subtitle2=The system covers only little space so it can be used even in small labs|size=medium}}
  
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To supervise the experiments we developed the software named MasterControlProgram or MCP. A single program is used to control the individual components of the bioreactor as well as analyse the data. The MCP can incorporate various components by following the protocol laid out.
  
  
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We focused on building a highly modular system. Requirements of the lab equipment can change depending on the experiment. Therefore it is not very useful to create a closed system that can do only one task. It should not matter if you need six pumps or just three analysis units or three complete reactors. With our system that is all possible and even more: The open implementation of the MCP makes it possible to easily add new components and be prepared for future requirements.
  
 
=References=
 
=References=

Revision as of 23:47, 18 September 2015

frist heading

As our cloning tools get more and more advanced, the next bottleneck starts to show up: strain characterization.

For metabolic engineering the chemostat cultivation is one of the most important characterization experiments. But not only are 200-1000 ml chemostats expensive to run, they are also not easy to standardize.


To increase the effectiveness of metabolic engineering research, we made a Do-It-Yourself chemostat bioreactor in the 10 ml scale.


In addition to that we want to do our bit in spreading biotechnology. At the moment professional lab equipment is not affordable for smaller institutions like schools, community labs and even small universities. If synthetic biology should be propagated in a wider public, it is necessary that its education starts early. Therefore our reactor system is DIY and inexpensive, so that it can be assembled in schools.

Aachen Reactor Overview 4.JPG
Overview over the whole system
The reactors with all tubes connected can be autoclaved while assembled and set up staying sterile afterwards
Aachen Reactor Overview 3.JPG
OD device and pump box
The system covers only little space so it can be used even in small labs

To supervise the experiments we developed the software named MasterControlProgram or MCP. A single program is used to control the individual components of the bioreactor as well as analyse the data. The MCP can incorporate various components by following the protocol laid out.


We focused on building a highly modular system. Requirements of the lab equipment can change depending on the experiment. Therefore it is not very useful to create a closed system that can do only one task. It should not matter if you need six pumps or just three analysis units or three complete reactors. With our system that is all possible and even more: The open implementation of the MCP makes it possible to easily add new components and be prepared for future requirements.

References