Difference between revisions of "Team:Brasil-USP"

 
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      Brasil-USP team's 2015 project is to develop an innovative
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      Our project is to develop an innovative
 
               and sustainable solution for a significant environmental
 
               and sustainable solution for a significant environmental
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               problem: <br /><span class="TextoVerde">accumulation of
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               problem: <br /><span class="TextoVerde"><b>accumulation of
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waste tires</span>
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waste tires</b></span>
 
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<h1>Both of these processes will be scaled up in bioreactors!</h1>
 
<h1>Both of these processes will be scaled up in bioreactors!</h1>
 
<img src="https://static.igem.org/mediawiki/2015/9/9f/Team-Brasil-USP_CapaReator.png" />
 
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             <h2>Our Parts</h2>
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             <h2>Our Project</h2>
 
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      Our team has submitted two different new parts to iGEM registry system: Lcp and RoxA, enzymes involved in natural rubber degradation. Also, we have worked hard characterizing our main DNA circuit. One of the used designs for this was a simple circuit where TetR inhibits GFP production. As our kill switch system is based on TetR inhibition, this experiment provided us key results to better understand and to model it, generating information on the timing balance and promoter strength of our main circuit.
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      Our project was designed to combat a serious environmental problem worldwide: the accumulation of rubber products waste. For this end, we designed a simple DNA circuit to be expressed in a model bacteria, E.coli. The designed DNA circuit will co-express two essential enzymes for rubber degradation, Lcp and RoxA, followed by their exportation – guaranteeing maximum contact with the threated materials.
 
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      <a class="btn btn-primary" href="#" role="button">Learn
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      <a class="btn btn-primary" href="https://2015.igem.org/Team:Brasil-USP/Project/Overview" role="button">Learn
 
more &raquo;</a>
 
more &raquo;</a>
 
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             <h2>Our main circuit</h2>
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             <h2>Entrepreneurship</h2>
 
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      Our project was designed to combat a serious environmental problem worldwide: the accumulation of rubber products waste. For this end, we designed a simple DNA circuit to be expressed in a model bacteria, E.coli. The designed DNA circuit will co-express two essential enzymes for rubber degradation, Lcp and RoxA, followed by their exportation – guaranteeing maximum contact with the threated materials. Both of these enzymes interact with the polymers composing natural rubber, generating several products. Between these, the most abundant is ODTD, a triisoprene unit that can be transformed into a high value fuel. Therefore, our project doesn’t only removes rubber waste from the environment, but it also turns it into a product with high aggregate value.  
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      Given the high potential for an industrial application, we have investigated possible business strategies that could turn our project into a competitive startup. As a first step, we have met with warehouses where waste tires are treated and discarded to both promote our ideas and learn more on how to implement our system into their reality. Furthermore, we applied an circular business model.  
 
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      <a class="btn btn-primary" href="#" role="button">Learn
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      <a class="btn btn-primary" href="https://2015.igem.org/Team:Brasil-USP/Entrepreneurship" role="button">Learn
 
more &raquo;</a>
 
more &raquo;</a>
 
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             <h2>Interlab Study</h2>
 
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      Given the high potential for an industrial application,
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      We also contributed with the Interlab Study, using DH5α E Coli with a backbone plasmid pSB1C3 to measure the promter strengths of three promoters from the Anderson's library. We showed differences and similarities when compared to the original experiments.
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      we have investigated possible business plans that could
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      turn our project into a competitive startup. As a first
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      step, we have met with warehouses where waste tires are
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      treated and discarded to both promote our ideas and
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      learn more on how to implement our system into their
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      reality. Several chemical processes involved in our
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      project are indeed complex, demanding some extra sfatety
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      care.
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more &raquo;</a>
 
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           <h1>We thank our sponsors for all support!</h1>
 
           <h1>We thank our sponsors for all support!</h1>
 
   
 
   
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           <p><a class="btn btn-primary btn-lg" href="/Team:Brasil-USP/Sponsors" role="button">Learn more &raquo;</a></p>
 
           <p><a class="btn btn-primary btn-lg" href="/Team:Brasil-USP/Sponsors" role="button">Learn more &raquo;</a></p>
 
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         <p><a class="btn btn-primary btn-lg" href="Team:Brasil-USP/Team/Members" role="button">Check out our team...&raquo;</a></p>
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         <p><a class="btn btn-primary btn-lg" href="/Team:Brasil-USP/Team/Members" role="button">Check out our team...&raquo;</a></p>
 
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Latest revision as of 00:53, 21 November 2015

Degradation of Natural Rubber

Team Brasil-USP

Our project is to develop an innovative and sustainable solution for a significant environmental problem:
accumulation of waste tires

Current recycling techniques demand high energy consumption, and are completely overwhelmed by the annual amount of rubber waste. In addition, natural rubber degradation may take up to 1000 years.

We aim to accelerate this process by genetically engineering microorganisms to express and secrete two crucial enzymes for the degradation of natural rubber: RoxA (Rubber oxygenase) and Lcp (Latex clearing protein).

Tires and several other products, however, are composed by vulcanized rubber, which ensures its durability and increase its elasticity and strength. In this regard, for vulcanized rubber degradation, the project may include a pre-treatment, which uses Acidithiobacillus ferrooxidans, bacteria that naturally devulcanizes rubber. Both of these processes will be scaled up in bioreactors.

Both of these processes will be scaled up in bioreactors!

Generic placeholder image

Our Project

Our project was designed to combat a serious environmental problem worldwide: the accumulation of rubber products waste. For this end, we designed a simple DNA circuit to be expressed in a model bacteria, E.coli. The designed DNA circuit will co-express two essential enzymes for rubber degradation, Lcp and RoxA, followed by their exportation – guaranteeing maximum contact with the threated materials.

Learn more »

Generic placeholder image

Entrepreneurship

Given the high potential for an industrial application, we have investigated possible business strategies that could turn our project into a competitive startup. As a first step, we have met with warehouses where waste tires are treated and discarded to both promote our ideas and learn more on how to implement our system into their reality. Furthermore, we applied an circular business model.

Learn more »

Generic placeholder image

Interlab Study

We also contributed with the Interlab Study, using DH5α E Coli with a backbone plasmid pSB1C3 to measure the promter strengths of three promoters from the Anderson's library. We showed differences and similarities when compared to the original experiments.

Learn more »

We thank our sponsors for all support!

Learn more »

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