Difference between revisions of "Team:Brasil-USP"

 
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{{Brasil-USP}}
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{{:Team:Brasil-USP/Templates/Head}}
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<html>
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<h2> Welcome to iGEM 2015!</h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h2> Project Description </h2>
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<html>
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<p align="justify">     Brasil-USP team`s 2015 project is to develop an innovative and sustainable solution for a significant environmental problem: accumulation of waste tires. Current recycling techniques demand high energy consumption, and are completely overwhelmed by the annual amount of rubber waste. In addition, natural rubber degradation may take up to 1000 years. We aim to accelerate this process by genetically engineering microorganisms to express and secrete two crucial enzymes for the degradation of natural rubber: RoxA (Rubber oxygenase) and Lcp (Latex clearing protein),. Tires and several other products, however, are composed by vulcanized rubber, which ensures its durability and increase its elasticity and strength. In this regard, for vulcanized rubber degradation, the project may include a pre-treatment, which uses Acidithiobacillus ferrooxidans, bacteria that naturally devulcanizes rubber. Both of these processes will be scaled up in bioreactors.
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The major resulting compound of the designed process is ODTD (12-oxo-4,8-dimethyl-trideca-4,8-diene-1-al), a triisoprene unit that can be turned into products with high commercial interest. Theoretical studies performed by our team indicate that it is possible to chemically or enzymatically transform this molecule into a fuel or a new polymer. In this year’s project, we propose a mechanism to alter this molecule into a highly functional compound. Consequently, besides enabling faster rubber degradation and decreasing final pollutant emission, our project also grants the final product a considerable economic interest.
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     <!-- Brasil-USP INTRODUCTION
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</p>
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==============================================
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      -->
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    <div class="container marketing">
  
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<h4>Before you start: </h4>
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      <!-- START THE FEATURETTES -->
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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</ul>
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<div class="highlightBox">
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      <div class="row featurette" style="padding-top: 40px; padding-bottom: 200px;">
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<h4> Styling your wiki </h4>
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<div class="container">
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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          <div class="col-md-7">
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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            <h2 class="featurette-heading center">
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</div>
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      Our project is to develop an innovative
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              and sustainable solution for a significant environmental
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              problem: <br /><span class="TextoVerde"><b>accumulation of
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waste tires</b></span>
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    </h2>
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            <p class="lead">
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      Current recycling techniques demand high energy
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      consumption, and
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      are <span class="TextoVermelho"><b>completely overwhelmed
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  by the annual amount of rubber waste</b></span>. In
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      addition, natural rubber degradation may take up to 1000
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      years.
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    </p>
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          </div>
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          <div class="col-md-5">
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    <div id="videoCover_">
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              <video width="450" id="videoCover" autoplay muted loop>
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<source src="https://static.igem.org/mediawiki/2015/1/1d/Team-Brasil-USP_VideoPneus.webm" type="video/webm" />
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<!--<source src="https://static.igem.org/mediawiki/2015/b/bd/Team-Brasil-USP_VideoPneus.mp4" type="video/mp4" />
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  -->              <source src="https://static.igem.org/mediawiki/2015/8/87/Team-Brasil-USP_VideoPneus.mov" type="video/mov">
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  Your browser does not support the video element.
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              </video>
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            </div>
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          </div>
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</div>
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      </div>
  
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<h4> Editing your wiki </h4>
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      <div class="row meio featurette" style="padding-top: 300px; padding-bottom: 300px;">
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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<div class="container">
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:Brasil-USP&action=edit"> Click here to edit this page! </a></p>
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          <div class="col-md-7 col-md-push-5">
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>  
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            <h2 class="featurette-heading">
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      We aim to accelerate this process by genetically
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      engineering microorganisms to express and secrete two
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      crucial enzymes for the degradation of natural
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      rubber: <span class="TextoAzul"><b>RoxA</b></span>
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      (Rubber oxygenase)
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      and <span class="TextoAzul"><b>Lcp</b></span> (Latex
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      clearing protein).
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    </h2>
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    <p class="lead">
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      Tires and several other products, however, are composed
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      by vulcanized rubber, which ensures its durability and
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      increase its elasticity and strength. In this regard,
 +
      for vulcanized rubber degradation, the project may
 +
      include a pre-treatment, which uses Acidithiobacillus
 +
      ferrooxidans, bacteria that naturally devulcanizes
 +
      rubber. Both of these processes will be scaled up in
 +
      bioreactors.
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    </p>  
 +
          </div>
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          <div class="col-md-5 col-md-pull-7">
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    <img src="https://static.igem.org/mediawiki/2015/4/4b/Team-Brasil-USP_CapaDesenho.png" style="height: 500px; margin-top: -50px;" />
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          </div>
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</div>
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      </div>
  
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      <div class="row featurette last" style="padding-top: 300px;padding-bottom: 300px;">
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<h1>Both of these processes will be scaled up in bioreactors!</h1>
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<img src="https://static.igem.org/mediawiki/2015/9/9f/Team-Brasil-USP_CapaReator.png" />
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      </div>
  
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<h4>Templates </h4>
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      <hr class="featurette-divider">
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the  
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>  
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 +
      <div class="row threecolumns">
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        <div class="col-lg-4">
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          <img  src="https://static.igem.org/mediawiki/2015/7/7d/Team-Brasil-USP_MainLogo.png" alt="Generic placeholder image" width="200">
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  <div class="board">
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            <h2>Our Project</h2>
 +
            <p>
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      Our project was designed to combat a serious environmental problem worldwide: the accumulation of rubber products waste. For this end, we designed a simple DNA circuit to be expressed in a model bacteria, E.coli. The designed DNA circuit will co-express two essential enzymes for rubber degradation, Lcp and RoxA, followed by their exportation – guaranteeing maximum contact with the threated materials.
 +
    </p>
 +
            <p>
 +
      <a class="btn btn-primary" href="https://2015.igem.org/Team:Brasil-USP/Project/Overview" role="button">Learn
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more &raquo;</a>
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    </p>
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          </div>
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</div><!-- /.col-lg-4 -->
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        <div class="col-lg-4">
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          <img src="https://static.igem.org/mediawiki/2015/thumb/9/9d/Team-Brasil-USP_LOGO3MODIFICADO.png/800px-Team-Brasil-USP_LOGO3MODIFICADO.png" alt="Generic placeholder image" width="290">
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  <div class="board">
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            <h2>Entrepreneurship</h2>
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            <p>
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      Given the high potential for an industrial application, we have investigated possible business strategies that could turn our project into a competitive startup. As a first step, we have met with warehouses where waste tires are treated and discarded to both promote our ideas and learn more on how to implement our system into their reality. Furthermore, we applied an circular business model.
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    </p>
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    <p>
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      <a class="btn btn-primary" href="https://2015.igem.org/Team:Brasil-USP/Entrepreneurship" role="button">Learn
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more &raquo;</a>
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    </p>
 +
          </div>
 +
</div>
 +
       
 +
        <div class="col-lg-4">
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          <img src="https://static.igem.org/mediawiki/2015/8/84/Home_Brasil_USP_globe.png" alt="Generic placeholder image" width="105">
 +
  <div class="board">
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            <h2>Interlab Study</h2>
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            <p>
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      We also contributed with the Interlab Study, using DH5α E Coli with a backbone plasmid pSB1C3 to measure the promter strengths of three promoters from the Anderson's library. We showed differences and similarities when compared to the original experiments.
 +
    </p>
 +
            <p>
 +
      <a class="btn btn-primary" href="https://2015.igem.org/Team:Brasil-USP/interlabstudy" role="button">Learn
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more &raquo;</a>
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          </div>
 +
  </p>
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        </div><!-- /.col-lg-4 -->
 +
      </div><!-- /.row -->
  
  
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<h4>Tips</h4>
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      <div class="sponsors">
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<div class="container">
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<ul>
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          <h1>We thank our sponsors for all support!</h1>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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  <img src="https://static.igem.org/mediawiki/2015/4/4c/BrasilUSP_sponsors_logos.png" />
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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          <p><a class="btn btn-primary btn-lg" href="/Team:Brasil-USP/Sponsors" role="button">Learn more &raquo;</a></p>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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</div>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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      </div>
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<li>Have lots of fun! </li>
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</ul>  
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<h4>Inspiration</h4>
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    </div><!-- /.container -->
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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<h4> Uploading pictures and files </h4>
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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    <div id="team">
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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      <div class="container">
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        <p><a class="btn btn-primary btn-lg" href="/Team:Brasil-USP/Team/Members" role="button">Check out our team...&raquo;</a></p>
 +
      </div>
 +
    </div>
  
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<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
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</html>
  
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{{:Team:Brasil-USP/Templates/Foot}}
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</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
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</html>
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Latest revision as of 00:53, 21 November 2015

Degradation of Natural Rubber

Team Brasil-USP

Our project is to develop an innovative and sustainable solution for a significant environmental problem:
accumulation of waste tires

Current recycling techniques demand high energy consumption, and are completely overwhelmed by the annual amount of rubber waste. In addition, natural rubber degradation may take up to 1000 years.

We aim to accelerate this process by genetically engineering microorganisms to express and secrete two crucial enzymes for the degradation of natural rubber: RoxA (Rubber oxygenase) and Lcp (Latex clearing protein).

Tires and several other products, however, are composed by vulcanized rubber, which ensures its durability and increase its elasticity and strength. In this regard, for vulcanized rubber degradation, the project may include a pre-treatment, which uses Acidithiobacillus ferrooxidans, bacteria that naturally devulcanizes rubber. Both of these processes will be scaled up in bioreactors.

Both of these processes will be scaled up in bioreactors!

Generic placeholder image

Our Project

Our project was designed to combat a serious environmental problem worldwide: the accumulation of rubber products waste. For this end, we designed a simple DNA circuit to be expressed in a model bacteria, E.coli. The designed DNA circuit will co-express two essential enzymes for rubber degradation, Lcp and RoxA, followed by their exportation – guaranteeing maximum contact with the threated materials.

Learn more »

Generic placeholder image

Entrepreneurship

Given the high potential for an industrial application, we have investigated possible business strategies that could turn our project into a competitive startup. As a first step, we have met with warehouses where waste tires are treated and discarded to both promote our ideas and learn more on how to implement our system into their reality. Furthermore, we applied an circular business model.

Learn more »

Generic placeholder image

Interlab Study

We also contributed with the Interlab Study, using DH5α E Coli with a backbone plasmid pSB1C3 to measure the promter strengths of three promoters from the Anderson's library. We showed differences and similarities when compared to the original experiments.

Learn more »

We thank our sponsors for all support!

Learn more »

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