Difference between revisions of "Team:Brasil-USP"

 
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    <!-- Brasil-USP INTRODUCTION
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<div id="Br"><a href=""><img src="https://static.igem.org/mediawiki/2015/e/e0/Team-Brasil-USP_CapaTopo_small.png" height="45px" /></a></div>
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      <!-- START THE FEATURETTES -->
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<div id="logo"><a href=""> iGEM Brasil-USP </a></div>
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    <li><a href="#capa" class="active" title="capa" >Home</a></li>
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            <h2 class="featurette-heading center">
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    <li><a href="#project" title="project">The Project</a></li>
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      Our project is to develop an innovative
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    <li><a href="#wiki" title="wiki">Our Wiki</a></li>
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              and sustainable solution for a significant environmental
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    <li><a href="#sponsors" title="sponsors">The Sponsors</a></li>
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              problem: <br /><span class="TextoVerde"><b>accumulation of
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    <li><a href="#team" title="team">The Team</a></li>
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waste tires</b></span>
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            <p class="lead">
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      Current recycling techniques demand high energy
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      consumption, and
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      are <span class="TextoVermelho"><b>completely overwhelmed
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  by the annual amount of rubber waste</b></span>. In
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      addition, natural rubber degradation may take up to 1000
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      years.
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<img class="igem" align="center" src="https://static.igem.org/mediawiki/2015/f/f7/Team-Brasil-USP_CapaMainsymbol.png" />
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            <h2 class="featurette-heading">
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<h2>Degradation of</h2>
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      We aim to accelerate this process by genetically
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<h1>Natural Rubber</h1>
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      engineering microorganisms to express and secrete two
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<div id="square">
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      crucial enzymes for the degradation of natural
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  <h3>Team Brasil-USP</h3>
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      rubber: <span class="TextoAzul"><b>RoxA</b></span>
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      (Rubber oxygenase)
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      and <span class="TextoAzul"><b>Lcp</b></span> (Latex
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      clearing protein).
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    </h2>
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    <p class="lead">
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      Tires and several other products, however, are composed
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      by vulcanized rubber, which ensures its durability and
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      increase its elasticity and strength. In this regard,
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      for vulcanized rubber degradation, the project may
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      include a pre-treatment, which uses Acidithiobacillus
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      ferrooxidans, bacteria that naturally devulcanizes
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      rubber. Both of these processes will be scaled up in
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      bioreactors.
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    </p>
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          <div class="col-md-5 col-md-pull-7">
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    <img src="https://static.igem.org/mediawiki/2015/4/4b/Team-Brasil-USP_CapaDesenho.png" style="height: 500px; margin-top: -50px;" />
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    <div id="Intro">
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      <div class="row featurette last" style="padding-top: 300px;padding-bottom: 300px;">
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<h1>Both of these processes will be scaled up in bioreactors!</h1>
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<img src="https://static.igem.org/mediawiki/2015/9/9f/Team-Brasil-USP_CapaReator.png" />
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      </div>
  
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            <h2>Our Project</h2>
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      Our project was designed to combat a serious environmental problem worldwide: the accumulation of rubber products waste. For this end, we designed a simple DNA circuit to be expressed in a model bacteria, E.coli. The designed DNA circuit will co-express two essential enzymes for rubber degradation, Lcp and RoxA, followed by their exportation – guaranteeing maximum contact with the threated materials.
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    </p>
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            <p>
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      <a class="btn btn-primary" href="https://2015.igem.org/Team:Brasil-USP/Project/Overview" role="button">Learn
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more &raquo;</a>
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          <img src="https://static.igem.org/mediawiki/2015/thumb/9/9d/Team-Brasil-USP_LOGO3MODIFICADO.png/800px-Team-Brasil-USP_LOGO3MODIFICADO.png" alt="Generic placeholder image" width="290">
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  <div class="board">
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            <h2>Entrepreneurship</h2>
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            <p>
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      Given the high potential for an industrial application, we have investigated possible business strategies that could turn our project into a competitive startup. As a first step, we have met with warehouses where waste tires are treated and discarded to both promote our ideas and learn more on how to implement our system into their reality. Furthermore, we applied an circular business model.  
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    </p>
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    <p>
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      <a class="btn btn-primary" href="https://2015.igem.org/Team:Brasil-USP/Entrepreneurship" role="button">Learn
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more &raquo;</a>
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    </p>
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          </div>
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</div>
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        <div class="col-lg-4">
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          <img src="https://static.igem.org/mediawiki/2015/8/84/Home_Brasil_USP_globe.png" alt="Generic placeholder image" width="105">
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  <div class="board">
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            <h2>Interlab Study</h2>
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            <p>
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      We also contributed with the Interlab Study, using DH5α E Coli with a backbone plasmid pSB1C3 to measure the promter strengths of three promoters from the Anderson's library. We showed differences and similarities when compared to the original experiments.
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    </p>
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            <p>
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      <a class="btn btn-primary" href="https://2015.igem.org/Team:Brasil-USP/interlabstudy" role="button">Learn
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more &raquo;</a>
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          </div>
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  </p>
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        <h3 class="title">
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            Brasil-USP team's 2015 project is to develop an <br />
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      <div class="sponsors">
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            innovative and sustainable solution for a significant environmental problem:
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<div class="container">
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            <br /><span class="TextoVerde">accumulation of waste tires</span>.
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          <h1>We thank our sponsors for all support!</h1>
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        </h3>
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  <img src="https://static.igem.org/mediawiki/2015/4/4c/BrasilUSP_sponsors_logos.png" />
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        <br />
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<h3>Current recycling techniques demand high energy consumption, and are <span class="TextoVermelho"><b>completely overwhelmed by the annual amount of rubber waste</b></span>. In addition, natural rubber degradation may take up to 1000 years.</h3>  
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          <p><a class="btn btn-primary btn-lg" href="/Team:Brasil-USP/Sponsors" role="button">Learn more &raquo;</a></p>
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            <br /><br /><br />
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    <h3>We aim to accelerate this process by genetically engineering microorganisms to express and secrete two crucial enzymes for the degradation of natural rubber: <span class="TextoAzul"><b>RoxA</b></span> (Rubber oxygenase) and <span class="TextoAzul"><b>Lcp</b></span> (Latex clearing protein).</h3>
+
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    <h3>Tires and several other products, however, are composed by vulcanized rubber, which ensures its durability and increase its elasticity and strength. In this regard, for vulcanized rubber degradation, the project may include a pre-treatment, which uses Acidithiobacillus ferrooxidans, bacteria that naturally devulcanizes rubber. Both of these processes will be scaled up in bioreactors.</h3>
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        <h3 class="title" style="color: #333;">Both of these processes will be scaled up in bioreactors!</h3>
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        <img src="https://static.igem.org/mediawiki/2015/9/9f/Team-Brasil-USP_CapaReator.png" height="400" style="display:block; margin: 0 auto 0 auto;" />
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      <h1>Very soon we will share our progress and results!</h1>
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      <img src="https://static.igem.org/mediawiki/2015/f/f4/Team-Brasil-USP_CapaFotosTime.jpg" />
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      <h1>These are our sponsors</h1>
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      <img src="https://static.igem.org/mediawiki/2015/4/4c/Team-Brasil-USP_CapaSponsors.png" />
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<h1>
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    We are students and post docs from<br />
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    University of São Paulo, Brazil
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<h2>
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    Learn more about our team!
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        <p><a class="btn btn-primary btn-lg" href="/Team:Brasil-USP/Team/Members" role="button">Check out our team...&raquo;</a></p>
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{{:Team:Brasil-USP/Templates/Foot}}

Latest revision as of 00:53, 21 November 2015

Degradation of Natural Rubber

Team Brasil-USP

Our project is to develop an innovative and sustainable solution for a significant environmental problem:
accumulation of waste tires

Current recycling techniques demand high energy consumption, and are completely overwhelmed by the annual amount of rubber waste. In addition, natural rubber degradation may take up to 1000 years.

We aim to accelerate this process by genetically engineering microorganisms to express and secrete two crucial enzymes for the degradation of natural rubber: RoxA (Rubber oxygenase) and Lcp (Latex clearing protein).

Tires and several other products, however, are composed by vulcanized rubber, which ensures its durability and increase its elasticity and strength. In this regard, for vulcanized rubber degradation, the project may include a pre-treatment, which uses Acidithiobacillus ferrooxidans, bacteria that naturally devulcanizes rubber. Both of these processes will be scaled up in bioreactors.

Both of these processes will be scaled up in bioreactors!

Generic placeholder image

Our Project

Our project was designed to combat a serious environmental problem worldwide: the accumulation of rubber products waste. For this end, we designed a simple DNA circuit to be expressed in a model bacteria, E.coli. The designed DNA circuit will co-express two essential enzymes for rubber degradation, Lcp and RoxA, followed by their exportation – guaranteeing maximum contact with the threated materials.

Learn more »

Generic placeholder image

Entrepreneurship

Given the high potential for an industrial application, we have investigated possible business strategies that could turn our project into a competitive startup. As a first step, we have met with warehouses where waste tires are treated and discarded to both promote our ideas and learn more on how to implement our system into their reality. Furthermore, we applied an circular business model.

Learn more »

Generic placeholder image

Interlab Study

We also contributed with the Interlab Study, using DH5α E Coli with a backbone plasmid pSB1C3 to measure the promter strengths of three promoters from the Anderson's library. We showed differences and similarities when compared to the original experiments.

Learn more »

We thank our sponsors for all support!

Learn more »

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