Difference between revisions of "Team:China Tongji/test"

 
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        <li class="listOne"><p id="listSafety">1. Safety</p></li>
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            <div class="titleButton"><a href="https://2015.igem.org/Team:China_Tongji/Achivement">Achivement</a></div>
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                    <p class="imgsName">Figure1: We use ethanol to sterilize our gloves before the experiments.</p>
 +
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 +
                <li><img height="533" src="https://static.igem.org/mediawiki/2015/8/89/China-Tongji-safety-figure2.jpg">
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                    <p class="imgsName">Figure2: We prevent the C.elegans incubator from being directly exposed to the environment.</p>
 +
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 +
                <li><img height="533" src="https://static.igem.org/mediawiki/2015/3/3e/China-Tongji-safety-figure3.jpg">
 +
                    <p class="imgsName">Figure3:  We use UV light to sterilize the laminar bench everytime we finish our experiments.</p>
 +
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 +
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                    <p class="imgsName">Figure4: We did most of the experiments on C.elegans and E.colis in the  laminar flow bench.</p>
 +
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                <li class=""><a>4</a></li>
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         <!-- 1. Safety -->
      <p class="titleOne" id="ProjectIntroduction">Project Introduction</p>   
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        <p class="titleOne" id="SafetySub">1. Safety</p>   
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         <p class="contentP">Safety is prior to all other stuffs especially in the lab.</p>
      <p class="contentP">In our project, we will use the technology of optogenetic and use light of different wave produced by the special light source assembled by ourselves to control the moving of C.elegans, construct a movement controlling system and bulid an amusement park of C.elegans.</p>
+
<p class="contentP">The Administrative office of Laboratory and Equipment in Tongji University is responsible for the safety of biology labs. Before we freshmen get into the lab and do experiments, we must take some courses offered by Administrative office of Laboratory and Equipment and have several tests to qualify our safety training on <a class="linkContent" href="http://aqjy.tongji.edu.cn" target="_blank">the Tongji University website</a>. </p>
<p class="contentP">We will try to design our special parts and express channalrhodopsin in specific C.elegans' neurons. To accomplish our goals to express channalrhodopsin in single neuron, we make the use of cre- loxp system and the overlapping of promoters. We not only use the traditional channalrhodopsin,chR2,but also try to express the novel channelrhodopsin, Blink, and other fancy channelrhodopsins which have never been tested in C.elegans.</p>
+
<p class="contentP">After taking the cources and passing the tests, we became familiar with some rules to secure the biosafety such as wearing the white coat and gloves is the necessity before doing the experiment, never bring the experiment materials out of the lab and never eat or drink inside the lab. And later all our members also sign up on a letter of commitment in which we promise to obey all the rules to secure the lab safety and the lab manager has the right to prevent us from continuing the experiments if we did something harmful to safety. </p>  
<p class="contentP">And we will also get some parts and assemble those parts into a new equipment which can serves as the light source of our experiment.Then we will use computer controlling that lightsource to change the light which can activate or suppress the channelrhodopsin. By doing that, we can try to control the behaviours of C.elegans such as moving forwards or twisting more effectively.</p>
+
<p class="contentP">In our project, we use 2kinds of organism, C.elegans and E.colis. E.coli is ranked in the organisms of Level 1 risk goup and C.elegans is icluded in <a class="linkContent" href="https://2015.igem.org/Safety/WhiteList" target="_blank">the iGEM White List</a> which means that none of the organisms we are working will cause any threat to the environment.</p>
<p class="contentP">What's more,we will express GFP,YFP,mcherry in E.coli. By combining the color of microorgasims and C.elegans, we want to construct some interesting scenes to form a C.elegans' fancy world.</p>
+
<p class="contentP">Even though the E.coli and the C.elegans we work with are not harmful for the environment and health, we still assume that they may threaten the ecology, so we wear rubber gloves and lab coat when we do the experiments to keep it from direct touching with human body. We also do all the experiments with these living organism in the laminar flow bench to prevent those organism from getting into the outside air. After we do the experiments, we use NaclO solution to sterilizing waste such as the used agar plate and the used tips. What's more, everytime after we finish our work, we use UV light to sterilize the lab.</p>
<p class="contentP">This technology will be helpful in the research on neuron's function and interaction. In the future, this technology may also be used in mechanical controlling system and the theraphy of movement defect.</p>
+
<p class="contentP">In order to secure 100 percetnt safe, we talked to the lab manager,Sanyun Liu, making sure that all of our experiments on E.coli are safe and also make sure the C.elegans we work with are properly disposed after our experiments.</p>
        <div class="divider"></div>
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<p class="contentP">To see some details about the safety of our parts, please look through <a class="linkContent" href="https://2015.igem.org/Safety/Final_Safety_Form?team_id=1634" target="_blank">our Safety Form</a>.</p>
       
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    <p class="titleOne" id="VideoAppreciation">Video Appreciation</p> 
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{{China_Tongji_Foot}}
 
{{China_Tongji_Foot}}

Latest revision as of 10:52, 14 September 2015

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Test

  • 1. Safety


  • 1. Safety

    Safety is prior to all other stuffs especially in the lab.

    The Administrative office of Laboratory and Equipment in Tongji University is responsible for the safety of biology labs. Before we freshmen get into the lab and do experiments, we must take some courses offered by Administrative office of Laboratory and Equipment and have several tests to qualify our safety training on the Tongji University website.

    After taking the cources and passing the tests, we became familiar with some rules to secure the biosafety such as wearing the white coat and gloves is the necessity before doing the experiment, never bring the experiment materials out of the lab and never eat or drink inside the lab. And later all our members also sign up on a letter of commitment in which we promise to obey all the rules to secure the lab safety and the lab manager has the right to prevent us from continuing the experiments if we did something harmful to safety.

    In our project, we use 2kinds of organism, C.elegans and E.colis. E.coli is ranked in the organisms of Level 1 risk goup and C.elegans is icluded in the iGEM White List which means that none of the organisms we are working will cause any threat to the environment.

    Even though the E.coli and the C.elegans we work with are not harmful for the environment and health, we still assume that they may threaten the ecology, so we wear rubber gloves and lab coat when we do the experiments to keep it from direct touching with human body. We also do all the experiments with these living organism in the laminar flow bench to prevent those organism from getting into the outside air. After we do the experiments, we use NaclO solution to sterilizing waste such as the used agar plate and the used tips. What's more, everytime after we finish our work, we use UV light to sterilize the lab.

    In order to secure 100 percetnt safe, we talked to the lab manager,Sanyun Liu, making sure that all of our experiments on E.coli are safe and also make sure the C.elegans we work with are properly disposed after our experiments.

    To see some details about the safety of our parts, please look through our Safety Form.