Team:China Tongji/Team
Team
1. Members
- 1.1 Xinhong Chen
- 1.2 Yu Feng
- 1.3 Geyang Zhang
- 1.4 Wenqi Cao
- 1.5 Zikang Xing
- 1.6 Yini Yang
- 1.7 Chenyu Pi
- 1.8 Shan qi
2. Advisor
- 2.1 Junwu Li
- 2.2 Yuanyuan Wu
3. Instructor
- 3.1 Songcheng Zhu
- 3.2 Wei Li
- 3.3 Meng Hao
4. Attributions
- 4.1 Team Members’ Attribution
- 4.2 Acknowledgement
5. Sponsor
- 5.1 Genewiz
- 5.2 Thorlabs
6. Contact
1. Members
Xinhong Chen
[ The leader of China_Tongji ]
A sophomore in Tongji University majoring in Life science.
Filled of crazy ideas, the “idea maker”.
Born in February, the most creative Aquarius.
Constantly exploring new fantasy.
Not easy to fall in love but single-minded when it happens.
Meet with optogenetic this summer, fall in love with C,elegans.
“Igemer” becomes my new mark.
Tough but memorable.
Yu Feng
[ The organizer of China_Tongji, also be in charge of team connection ]
A sophomore student who is major in life science.
Fall in love with biology when I was a high school student.
Always dreaming to be a neurobiologist and study of how the brain works.
Be a member of Tongji team last year as an intern student.
So proud to be an iGEMer and lead our team to finish the project this time!
Good at organizing the team and the whole project.
Thoughtfulness and carefulness is my major virtue.
9 months hardworking with my lovely friends, I hope we can get what we want in the final competition!
Geyang Zhang
[ C.elegans, the art director ]
I’m Geyang Zhang, a girl with song and sunshine;
China is my homeland, I value all the creatures it breeds;
Nature pushed me into science, I explore deep in its mind;
Grown up as time passed by, Tongji is a new place to play;
Then I meet with IGEM, where my ideas would be made;
Friends gathered for the same goal, we are pursuing for a medal of gold;
Worms became our road to go, and light in cells are things we want to know;
Some changes may be made by my hands, which is the most exciting thought filled my head.
Wenqi Cao
[ The expert in C.elegans’ microinjection ]
I am Wenqi cao. A girl.
Like reading, travelling, gardening but not microinjecting.
Like all lovely creatures including C.elegans.
Like the sky when sakura blossoming.
Like the wind when stars sparkling.
Especially like the microscope when worm fluorescing.
Dream to be a writer and travel to all the fascinating places in the world. And it sounds good to be a worm eating E.coli all the time till die.
Happy to be a member of Tongji iGem team.
Happy to work with my dear friends.
Happy to meet you, good luck to whoever you are.
Zikang Xing
[ Be in charge of Molecular Architectures ]
I am a Chinese, Masculinity is my gender.
My major is called bioscience, Science is my mentor.
I don't fear difficulty, Sometimes I like challenge.
I only want to keep happy, Optimism never change.
This year I join iGEM, Working hard with classments.
We set up a new team, Designing optogenetic C.elegans.
Medal is in our dreams, We will beat other teams!
Yini Yang
[ Be in charge of Molecular Architectures ]
I am a Chinese, Femininity is my gender.
My major is called bioscience, Science is my mentor.
My favorite is nature, I respect each individual.
Life is the rarest treasure, All units are equal.
I have special thought, My creativity never leave.
Idea comes from my heart, And I'll work hard to achieve!
People from other countries, I'd like to make friends with!
Chenyu Pi
[ Director of molecular biology, the leader of E.coli part ]
Hello, my name is Chenyu Pi.
I like to smile and I think smile is the best treasure that god give me.
Not only I love science, but also I love sports and music.
They may become all my life.
In our team, I concentrate on the molecular experiment such as PCR, transformation and ligation.
I always try hard to achieve a goal although the result does not conform to our expectation.
Come on, fighting!
Shan qi
[ Be in charge of LED light source building ]
My name is SHAN QI, and I am a student of Tongji University who is major in life science.
I am a big boy from the north district of China. I loves traditional Chinese culture and some Chinese style music.
As a student who is major in biology, I likes to find out the right thing.
I always insist that the best preparation for tomorrow is doing the best today.
It is my first year to join in the iGEM.
So I am looking forward to do something fancy in this competition.
2. Advisor
2.1 Junwu Li
Hi,I am Junwu Li. This is my first time to contact iGEM, I think it is a very meaningful competition.
I am very interested in programming, I think it is amazing to realize my ideas by computer language.
I feel honored to have chance to design Wiki page for Tongji University Team of China in 2015, meanwhile, I can learn a lot relevant knowledge in the process of designing web page.Anyway, I will try my best to design the wiki page, ensure that the content is easy to read and the interface is beautiful.
2.2 Yuanyuan Wu
My name is Yuanyuan Wu and I am 23 years old, born in Jiangsu province.
I was graduated from China Pharmceutical University.
My major is bioengineering and I got my bachelor degree after my graduation in the year of 2014.
I have lots of hobbies, for example, swimming, jogging, badminton.
I am open-minded, willing and have broad interests like reading and especially in financial market.
3. Instructor
3.1 Songcheng Zhu
I am Songcheng Zhu, an Associate Professor at Life School of Tongji University.I like music, travelling, sports and stay with my kids in my spare time.My major is Biochemistry and I love those molecules in the body of life, particularly DNA, RNA and Protein. I am very interesting in how a protein is located to the right place in the the right time in a cell.
Happy to be a teacher in An University, where I can work and study with young students.Even more happy to work with our iGEM team, it is a diligent and enthusiastic unit I have ever seen.
Good luck to our team.
3.2 Wei Li
Dr. Li joined the faculty of the School of Life Sciences and Technology in 2012. Li received her Ph.D. degree in Biophysics from Huazhong University of Science and Technology. Prior to joining Tongji University, Li completed her post-doctoral training at the University of Michigan, where she was awarded the American Heart Association Postdoctoral Fellowship.
The Li lab combines the genetic model organism C.elegans and mammalian system to study the mechanisms of mechanosensation and pain, and uses a multidisciplinary approach of molecular genetics, functional imaging, electrophysiology, optogenetics and behavioral analysis.
3.3 Meng Hao
Hey guys, this is Heidi saying hi from Shanghai. It's really a huge honor and pleasure for me to join the iGEM this year. Currently I work as an international program coordinator of School of Life Sciences and Technology, Tongji University and I will be the guiding teacher of the 2015 iGEM representative team of Tongji University. Though it is the first time for our team to participate in a global science competition, I have strong confidence in all of them that they would make a great difference here on their debut, with their extraordinary courage and dedication. While what really matters to us is nothing but the precious experience where we can see the world and gain growth. Can't wait to meet you all in Boston! Cheers!
4. Attributions
4.1 Team Members’ Attribution
4.1.1 Xinhong Chen
The team leader: Build our team in January.
Paperwork: Introduction and abstract of the project, About Our Lab Form, About Our Project Form, Final Safety Form, Part Registry.
Primer designing: Design all the primers for the seamless clone experiments in August.
Build our parts: Try to make pmec4-ic1c2, pmec4-dsred and pmec4-blink.
Make our backbone: pSB1C3-pmyo2-chR2, pSB1C3-pmyo2-dsRed, pSB1C3-pmyo3-chR2, pSB1C3-pmyo3-dsRed.
Help setting up the test model on the C.elegans.
Human practice: Give science class in Tongji Primary School to help them learn more about iGEM and Synthenic Biology.
Communicate with Tongji University to get some funding and experiment reagent.
Travel arrangement.
4.1.2 Yu Feng
Organize the team: Contact between the team and the school.
Paper work: Project budget, experiment record and all the funding application forms.
Build our parts: ptwk16-chR2, ptwk16-IC1C2 and ptwk16-chETA, pmyo3-ic1c2, pmyo3-chETA, pmyo2-ic1c2 and pmyo2-chETA.
Make our backbone: pSB1C3-ptwk16-chR2, pSB1C3-pmyo2-chR2, pSB1C3-pmyo2-chETA and pSB1C3-ptwk16-chETA.
Contact with other teams to get communication with them, including NJAU, SYSU, SJTU, ITB and NYU. Also contact with Alexander Gottschalk’ lab in Goethe University, Frankfort, Germany to get their help.
Find sponsors: Contact with GENEWIZ and THORLABS to get us some project fund.
Help design of wiki page: Write basic material for our wiki page. Including protocol of our experiments, lab note, attribution and team introduction.
Travel arrangement.
4.1.3 Geyang Zhang
The art director: Design of our logo, team suit, banner, video, poster and postcard.
Test our C.elegans: Do the behavior test every day and maintain our worms. Including pmyo3-chR2, pmyo2-chR2, pmyo2-ic1c2, pmyo2-chETA, pmyo3-ic1c2, pmyo3-chETA, pttx3-chR2, pttx3-ic1c2 and ptwk16-chR2.
Modeling: Use the test result to build our optogenetics model.
Contact with Anna Moroni’s lab in University of Milan to get their blink plasmid.
4.1.4 Wenqi Cao
Idea maker: Finally came out of our project idea: optogenetic on C.elegan.
Build our parts: pmyo3-chR2 and pmyo2-chR2.
Microinjection: Tried pmyo-2-chR2 , pmyo2-blink, pmyo2-iC1C2, pmyo2-cheTA, pmyo-3-chR2, pmyo3-blink, pmyo3-ic1c2, pmyo3-chETA, pmec-3-chR2,cre-loxP system and pttx-3-chR2.
Paperwork: Experiment design. Write a part of background which include the mechanism of ChR2 and why we choose those opsins.
Travel arrangement.
4.1.5 Shan Qi
The equipment director: Assemble the light sources and improve their performances.
Test our C.elegans: Do the behavior test every day and maintain our worms. Including pmyo3-chR2, pmyo2-chR2, pmyo2-ic1c2, pmyo2-chETA, pmyo3-ic1c2, pmyo3-chETA, pttx3-chR2, pttx3-ic1c2 and ptwk16-chR2.
Modeling: Draw the C.elegans track and analyze them.
Paperwork: Experiment design.
4.1.6 Chenyu Pi
Make our fluorescence E.coli: Design the experiment and make out YFP, GFP and mcherryPet22b E.coli, which we are going to use in the traffic model of C.elegan. Then try to make YFP and GFP OP50 and PA14, these are the food of C.elegan.
Build our parts: pmyo2-blink, pmyo3-blink, pmyo2-ic1c2, pmyo2-chETA, pttx3-chR2, pttx3-ic1c2, pttx3-blink and pttc3-dsred.
Make our backbone: pSB1C3-pmyo3-chR2, pSB1C3-pmyo2-chR2, pSB1C3-pmyo3-blink, pSB1C3-pmyo2-blink, pSB1C3-pttx3-blink, pSB1C3-pttx3-chR2 and pSB1C3-pttx3-dsred.
Paint the plasmid profile.
4.1.7 Zikang Xing
Design our questionnaire and analyze the result.
Build our parts: pttx3-chR2, pttx3-blink, pttx3-ic1c2, pttx3-chETA and pttx3-dsred.
Update the pmyo2-dered.
Make our backbone: pSB1C3-pttx3-chR2, pSB1C3-pttx3-blink, pSB1C3-pttx3-ic1c2, pSB1C3-pttx3-chETA and pSB1C3-pttx3-dsred.
Point mutation: Update the pmyo2-dered.
Help with the modeling build.
4.1.8 Yini Yang
Design our questionnaire and analyze the result.
Build our parts: pttx3-chR2, pttx3-blink, pttx3-ic1c2, pttx3-chETA and pttx3-dsred.
Update the pmyo2-dered.
Make our backbone: pSB1C3-pttx3-chR2, pSB1C3-pttx3-blink, pSB1C3-pttx3-ic1c2, pSB1C3-pttx3-chETA and pSB1C3-pttx3-dsred.
Paper work: Human practice and the standards of the different medals.
Primer design all the tradition method of ligation’s primers.
Help contact with some biology company in order to get some funding.
4.1.9 Advisor Junwu Li
Help us make our wiki, which allows us to present out project in a beautiful way.
Give us some advice about the data organization and formal paper work.
4.1.10 Advisor Yuanyuan Wu
Teach us how to do the microinjection on C.elegan.
Help us make two of our plasmids: ptwk16-blink and ptwk16-ic1c2.
4.2 Acknowledgement
Here, we want to thank for all professors, teachers and advisors who have offered us great help during our project.
4.2.1 General support
First and foremost, we would like to show our deepest gratitude to our instructors: professor Songcheng Zhu and professor Wei Li. Professor Zhu, a respectable, responsible and resourceful scholar, who has provided us the advices of the project’s idea and molecular experiments. As an experienced instructor of C.elegan, Professor Li helped us a lot on the worm part. Dr. Li has also offered us many suggestions on our equipment assembling.
We also want to thank for our instructor MengHao, who is going to take us to the Jamboree!
4.2.2 Lab support
Here, we want to thank for all the labs which provided us with the experiment reagent, including Professor Chao Zhang’s lab, Professor Wei Li’s lab, Professor Jianmin Fang’s lab, Professor Jian Fei’s lab and Professor Jiuhong Kang’s lab. We definitely couldn’t finish our project without their kind help!
4.2.3 Funding support
Thanks for Professor Jing Zhang, who is in charge of our project in school. She helped us a lot in buying reagent and equipment.
Thanks for our college---Tongji University, they gave us enough funding to start our project.
Also, we want to thank GENEWIZ and THORLABS, these two companies are our project’s sponsors.
4.2.4 Wiki support
We are grateful to our advisor Junwu Li who helped us on designing our wiki page. Because of him, we can show our project in such a beautiful way!
4.2.5 Technique support
Our advisor Yuanyuan Wu taught us how to do the microinjection on C.elegans patiently. She also taught us how to use the fluorescence microscope.
4.2.6 Human practice support
One of our human practice is the exhibition at Shanghai Science Museum, thanks to NYU, they contacted with the museum so that we can have this fantastic human practice.
We have also gave a popular science course at Tongji Primary School, thanks to Green Road Club which gave us this chance to let more young student know about synthetic biology.
4.2.7 Presentation coach
Professor Songcheng Zhu and professor Jing Zhang helped us on presentation coaching. Thanks for their patient instructions.
4.2.8 Plasmid support
Finally but no least, in our project, we used some other labs’ plasmids. Here we want to show our sincere thanks to these labs, which include Alexander Gottschalk’ lab in Goethe University, Frankfort, Germany and Anna Moroni’s lab in University of Milan, Milano. Thank you for your kindness!
5. Sponsor
5.1 GENEWIZ
Excelling in both science and service, GENEWIZ is the largest trusted partner for DNA services. Since 1999, our commitment is to advance research productivity through our distinctive combination of fast, reliable results, specialized expertise, competitive pricing, excellent project management and easy access to award-winning, friendly technical support.
With state-of-the-art facilities at the local, national, and international levels, we value our loyal customers who have made GENEWIZ an industry leader. Together, we can make a difference in the pursuit of scientific discoveries, better medicines, a greener environment, and abundant healthy food supplies.
It's easy to work with GENEWIZ!
5.2 THORLABS
Thorlabs, Inc. is a privately held optical equipment company headquartered in Newton, New Jersey. The company was founded by Alex Cable, who serves as its current president and CEO. As of 2013, Thorlabs employed about 1,000 people directly or indirectly and has sales of approximately $200 million.The company has offices in multiple American States, Brazil, Canada, China, France, Germany, Japan, Sweden, and the United Kingdom. It sells approximately 20,000 different products.
Thorlabsdesigns and manufactures products in the areas of breath analysis technology, fiber optics, lasers, optical instrumentation, optomechanics, photonics, and vibration isolation. Approximately 90% of the products it sells are manufactured in-house.
6. Contact
Xinhong Chen
Email: 1353325@tongji.edu.cn
Phone number: 86-15221397515
Address: NO.1239, Siping Road, Tongji University
Yu Feng
Email: hyfy1995@sina.com
Phone number: 86-15316722910
Address: NO.1239, Siping Road, Tongji University
Copyright © China_Tongji iGEM 2015 | Developer: LiJunWu