Difference between revisions of "Team:Czech Republic/Goals"

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==Golas of module 1==
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*Construct a set of reporter promoters for yeast cells
 +
*Characterize reporter promoters in all mating types
 +
*Design synthetic MATa and MATx strains
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*Build a synthetic diploid strain with a functional yeast pheromone pathway
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*Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids
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==Goals of module 2==
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* Construct a set of yeast plasmids with different mating pheromones and their receptors and contributed to the Registry with 6 BioBricks
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* Verify the correct coupling of the receptors to the yeast pheromone mating pathway
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* Verify the correct expression and secretion of the different pheromones
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* Show the orthogonality of the used receptors and pheromones
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Revision as of 18:31, 18 September 2015

Goals

Golas of module 1

  • Construct a set of reporter promoters for yeast cells
  • Characterize reporter promoters in all mating types
  • Design synthetic MATa and MATx strains
  • Build a synthetic diploid strain with a functional yeast pheromone pathway
  • Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids

Goals of module 2

  • Construct a set of yeast plasmids with different mating pheromones and their receptors and contributed to the Registry with 6 BioBricks
  • Verify the correct coupling of the receptors to the yeast pheromone mating pathway
  • Verify the correct expression and secretion of the different pheromones
  • Show the orthogonality of the used receptors and pheromones