Difference between revisions of "Team:Czech Republic/Goals"

(Goals of module 2)
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* Verify the correct expression and secretion of the different pheromones
 
* Verify the correct expression and secretion of the different pheromones
 
* Show the orthogonality of the used receptors and pheromones
 
* Show the orthogonality of the used receptors and pheromones
 +
==Goals of module 3==
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* Demonstrate the ability of our receptors to bind chosen markers.
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{{:Team:Czech_Republic/Template:Bottom}}

Revision as of 18:36, 18 September 2015

Goals

Golas of module 1

  • Construct a set of reporter promoters for yeast cells
  • Characterize reporter promoters in all mating types
  • Design synthetic MATa and MATx strains
  • Build a synthetic diploid strain with a functional yeast pheromone pathway
  • Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids

Goals of module 2

  • Construct a set of yeast plasmids with different mating pheromones and their receptors and contribute to the Registry with new BioBricks
  • Verify the correct coupling of the receptors to the yeast pheromone mating pathway
  • Verify the correct expression and secretion of the different pheromones
  • Show the orthogonality of the used receptors and pheromones

Goals of module 3

  • Demonstrate the ability of our receptors to bind chosen markers.