Team:Czech Republic/Goals

Goals

Golas of module 1

  • Construct a set of reporter promoters for yeast cells
  • Characterize reporter promoters in all mating types
  • Design synthetic MATa and MATx strains
  • Build a synthetic diploid strain with a functional yeast pheromone pathway
  • Demonstrate the correct functionality of yeast pheromone pathway in synthetic diploids

Goals of module 2

  • Construct a set of yeast plasmids with different mating pheromones and their receptors and contribute to the Registry with new BioBricks
  • Verify the correct coupling of the receptors to the yeast pheromone mating pathway
  • Verify the correct expression and secretion of the different pheromones
  • Show the orthogonality of the used receptors and pheromones