Difference between revisions of "Team:Czech Republic/Practices"
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The possitive attitude and further interest of many respondents really delighted us. | The possitive attitude and further interest of many respondents really delighted us. | ||
| − | However the difference in opinions between biologists and general public is disturbing althought anticipated. On that account we are going to arrange more lectures about synthetic biology on our | + | However the difference in opinions between biologists and general public is disturbing althought anticipated. On that account we are going to arrange more lectures about synthetic biology on our university and also for general public in Czech Republic. |
[[Team:Czech_Republic/Practices/GMO_survey|Read more about the survey...]] | [[Team:Czech_Republic/Practices/GMO_survey|Read more about the survey...]] | ||
Revision as of 10:22, 18 September 2015
Human Practice
Contents
Outreach strategy
The IOD band presents a new approach to diagnostics and diagnostic engineering. So at Team Czech Republic we addressed engineers and medical experts to discuss the application and the novel engineering approach. In addition, Czech Republic teamed up with Chalmers Gothenburg to conduct a multinational survey on public conception of Synthetic Biology.
Engineering
Since a lot of ideas behind IODs were build on the fundamentals of classical engineering, we wanted to present the concept of our project (from an engineering point-of-view) to young engineers and get their feedback. We had a great opportunity to present our project and synthetic biology in general at a lecture of Introduction into Cybernetics led by Prof. Ing. Miloš Schlegel CSc. After giving a 30 min lecture about synthetic biology, we introduced our project. The response was surprisingly positive. Not only were we asked several to-the-point questions, but several students even showed deeper interest in synthetic biology and individually contacted us later.
Key outputs
Since most of us are not only synthetic biologist but also students of cybernetics, engineering point of view strongly influenced design of our project.
Our engineering experience in modeling, system design and control was perceptible in all sections of IODs´ development.
Most engineers eager to see the immediate reaction of the system. Therefore we designed IODs as a fast diagnostic test. Also the preliminary process is quick thanks to the cloning-free preparation of required IODs.
Questions about current cancer diagnostics and their prizes and efficiency that we received during our lecture were thereafter discussed with cancer specialists in the interview.
Read more about the lecture...
Medicine
In order to get a professional opinion on our project and to learn more about cancer and CTCs, we conducted an interview with two cancer specialists, Mgr. Pavel Pitule, Ph.D. and Mgr. Pavel Ostašov, Ph.D. The interview took place at the Biomedical Center of Faculty of Medicine in Pilsen. After introduction of our project idea we discussed some particularly interesting points. This interview was not only helpful and enriching but also led to an agreement to collaborate with the Laboratory of Tumor Biology in the near future.
Key outputs
Use a change of color instead of the agglutination as an indicator of CTCs' presence
In response, a group of experiments from module 2 was changed to use GFP as an indicator of successful communication between IODs. The use of color indication in the further research proved advantageous (atleast partially), and the use of color indicators will be always considered in later experiments.
A recommendation to test the agglutination on a slide
In response, since we have the possibility to perform our experiments in microfluidics, we considered sticking by this advice and used microfluidics in our agglutination experiments.
Mgr. Pavel Pitule Ph.D.
Mgr. Pavel Pitule, Ph.D. is a junior researcher at the Biomedical Center at the Faculty of Medicine in Pilsen. He is the research group leader of Laboratory of Tumor Biology.
Mgr. Pavel Ostašov Ph.D.
Mgr. Pavel Ostašov, Ph.D. is a researcher at the Biomedical Center at the Faculty of Medicine in Pilsen. He is a member of Laboratory of Tumor Biology.
Multinational general public vs. biologists GMO survey
Team Chalmers Gothenburg offered us to participate in their GMO survey. We asked 63 biologists and 50 respondents from general public to fill in the survey. Same thing was done in Sweden. Thanks to that we have the opportunity to compare GMO knowledge in Sweden and Czech Republic. Big thanks belongs to team Chalmers Gothenburg for statistical evaluation of all data.
Key outputs
The possitive attitude and further interest of many respondents really delighted us.
However the difference in opinions between biologists and general public is disturbing althought anticipated. On that account we are going to arrange more lectures about synthetic biology on our university and also for general public in Czech Republic.
Safety
We discussed the safety issues of our technology throughout the whole duration of the project since we were aware of working with modified S. cerevisiae with the vision to use them in a clinical setting. Read more...