Difference between revisions of "Team:Czech Republic/Practices"

(Multinational general public vs. biologists GMO survey)
(Key outputs)
 
(18 intermediate revisions by 4 users not shown)
Line 1: Line 1:
{{:Team:Czech_Republic/Template:Top|Human Practice}}
+
{{:Team:Czech_Republic/Template:Top|Human Practices}}
  
 
= Outreach strategy =
 
= Outreach strategy =
The IOD band presents a new approach to diagnostics and diagnostic engineering. So at Team Czech Republic we addressed engineers and medical experts to discuss the application and the novel engineering approach. In addition, Czech Republic teamed up with Chalmers Gothenburg to conduct a multinational survey on public conception of Synthetic Biology.  
+
The IOD band presents a new approach to diagnostics and diagnostic engineering. So at Team Czech Republic we addressed engineers and medical experts to discuss the application and the novel engineering approach. In addition, Czech Republic teamed up with Chalmers Gothenburg to conduct a multinational survey on the public perception of Synthetic Biology.  
  
 
<html><div><div class="left"></html>
 
<html><div><div class="left"></html>
Line 9: Line 9:
  
 
[[File:Czech_Republic_IODLecture.png|470px|link=]]
 
[[File:Czech_Republic_IODLecture.png|470px|link=]]
Since a lot of ideas behind IODs were build on the fundamentals of classical engineering, we wanted to present the concept of our project (from an engineering point-of-view) to young engineers and get their feedback. We had a great opportunity to present our project and synthetic biology in general at a lecture of Introduction into Cybernetics led by Prof. Ing. Miloš Schlegel CSc. After giving a 30 min lecture about synthetic biology, we introduced our project. The response was surprisingly positive. Not only were we asked several to-the-point questions, but several students even showed deeper interest in synthetic biology and individually contacted us later.
 
  
 +
Since a lot of ideas behind IODs were built on the fundamentals of classical engineering, we wanted to present the concept of our project (from an engineering point-of-view) to young engineers and receive their feedback. We presented our project and synthetic biology in general at a lecture of the Introduction to Cybernetics class led by Prof. Ing. Miloš Schlegel CSc. After giving a 30-minute lecture on synthetic biology, we introduced our project. The response was surprisingly positive. Not only we were asked several to-the-point questions, but some of the students even showed a deeper interest in synthetic biology and individually contacted us after the talk.
  
===Key outputs===
+
<html></div><div class="right"></html>
Since most of us are not only synthetic biologist but also students of cybernetics, the engineering point of view strongly influenced our project design.
+
  
Our engineering experience in modeling, system design and control was perceptible in all sections of IOD development.
+
== [[Team:Czech_Republic/Practices/Interview|Medicine]] ==
 +
[[File:Czech_Republic_Interview_introduction.png|475px|link=]]
  
Most engineers are eager to see the immediate reaction of the system. Following the lectures such as the one for young engineers we designed IODs as a fast diagnostic test. Also the preliminary process is quick thanks to the clone-free preparation of required IODs.
+
In order to get a professional opinion on our project and to learn more about cancer and CTCs, we conducted an interview with two cancer specialists, Mgr. Pavel Pitule, Ph.D. and Mgr. Pavel Ostašov, Ph.D. The interview took place at the Biomedical Center of Faculty of Medicine in Pilsen. After introducing our project idea we discussed some particularly interesting points. This interview was not only helpful and enriching but also led to an agreement to collaborate with the Laboratory of Tumor Biology in the near future.
  
Questions we received during our lecture from students and teachers concerning current cancer diagnostics and their price and efficiency were afterwards discussed with cancer specialists in the [[Team:Czech_Republic/Practices/Interview| interview]].
 
  
 +
<html></div><div style="clear: both;"></div><div class="left"></html>
  
 +
===Key outputs=== 
 +
 +
Most engineers are eager to see the immediate reaction of the system. '''Following the lectures such as the one for young engineers, we designed IODs as a fast diagnostic test'''. Also, the preliminary process is quick thanks to the clone-free preparation of required IODs.
 +
 +
'''Questions we received''' during the lecture from students and teachers concerning current cancer diagnostics, their price, and efficiency '''were afterwards discussed with cancer specialists in the [[Team:Czech_Republic/Practices/Interview| interview]]'''.
  
 
[[Team:Czech_Republic/Practices/Lecture | Read more about the lecture...]]
 
[[Team:Czech_Republic/Practices/Lecture | Read more about the lecture...]]
Line 27: Line 32:
  
 
<html></div><div class="right"></html>
 
<html></div><div class="right"></html>
 
== [[Team:Czech_Republic/Practices/Interview|Medicine]] ==
 
[[File:Czech_Republic_Interview_introduction.png|475px|link=]]
 
In order to get a professional opinion on our project and to learn more about cancer and CTCs, we conducted an interview with two cancer specialists, Mgr. Pavel Pitule, Ph.D. and Mgr. Pavel Ostašov, Ph.D. The interview took place at the Biomedical Center of Faculty of Medicine in Pilsen. After introduction of our project idea we discussed some particularly interesting points. This interview was not only helpful and enriching but also led to an agreement to collaborate with the Laboratory of Tumor Biology in the near future.
 
  
 
=== Key outputs ===
 
=== Key outputs ===
  
'''Use a change of color instead of the agglutination as an indicator of CTCs' presence'''
+
'''We were recommended to use a color change instead of the agglutination as an indicator of CTCs' presence.'''
 +
As a result, a group of experiments from module 2 was changed to use GFP as an indicator of successful communication between IODs. The use of color indication in the further research proved advantageous (at least partially), and the use of color indicators will always be considered in later experiments.
  
In response, a group of experiments from module 2 was changed to use GFP as an indicator of successful communication between IODs. The use of color indication in the further research proved advantageous (atleast partially), and the use of color indicators will be always considered in later experiments.
 
  
 +
'''A recommendation was made to test the agglutination on a slide.'''
 +
As a result, since we had the possibility to perform our experiments in microfluidics, we listened to this advice and used microfluidics as well as microscope slides in our agglutination experiments.
  
'''A recommendation to test the agglutination on a slide'''
+
[[Team:Czech_Republic/Practices/Interview | Read more about the interview...]]
 
+
In response, since we have the possibility to perform our experiments in microfluidics, we considered sticking by this advice and used microfluidics in our agglutination experiments.
+
 
+
[[Team:Czech_Republic/Practices/Interview | Read the interview...]]
+
  
 
<html><div style="clear: both;"></html>
 
<html><div style="clear: both;"></html>
  
[[File:Czech_Republic_interview_Pitule.jpg|150px|left|border|link=|Interview]]
 
====Mgr. Pavel Pitule Ph.D.====
 
Mgr. Pavel Pitule, Ph.D. is a junior researcher at the Biomedical Center at the Faculty of Medicine in Pilsen. He is the research group leader of Laboratory of Tumor Biology.
 
 
<html></div><div style="clear: both;"></html>
 
 
[[File:Czech_Republic_interview_Ostasov.png|150px|left|border|link=|Interview]]
 
====Mgr. Pavel Ostašov Ph.D.====
 
Mgr. Pavel Ostašov, Ph.D. is a researcher at the Biomedical Center at the Faculty of Medicine in Pilsen. He is a member of Laboratory of Tumor Biology.
 
  
 
<html></div></div><div style="clear: both;"></div></div></html>
 
<html></div></div><div style="clear: both;"></div></div></html>
Line 61: Line 51:
 
= [[Team:Czech_Republic/Practices/GMO_survey|Multinational general public vs. biologists GMO survey]] =
 
= [[Team:Czech_Republic/Practices/GMO_survey|Multinational general public vs. biologists GMO survey]] =
  
Team Chalmers Gothenburg offered us to participate in their GMO survey. We asked 63 biologists and 50 respondents from general public to fill in the survey. Same thing was done in Sweden. Thanks to that we have the opportunity to compare GMO knowledge in Sweden and Czech Republic. Big thanks belongs to team Chalmers Gothenburg for statistical evaluation of all data.
+
Team Chalmers Gothenburg offered us to participate in their GMO survey. We asked 63 biologists and 50 respondents from the general public to fill in the survey. Same thing was done in Sweden. Thanks to this, we had the opportunity to compare GMO knowledge in Sweden and Czech Republic. Big thanks belongs to the team Chalmers Gothenburg for statistical evaluation of all data.
  
<h3>Key outputs</h3>
+
===Key outputs===
The possitive attitude and further interest of many respondents really delighted us.
+
The positive attitude and further interest of many respondents delighted us.
  
However the difference in opinions between biologists and general public is disturbing althought anticipated. On that account we are going to arrange more lectures about synthetic biology on our university and also for general public in Czech Republic.   
+
However, although anticipated, the difference in opinions between biologists and the general public is disturbing. On that account, we are going to arrange more lectures about synthetic biology at our university and also for the general public in Czech Republic.   
 
   
 
   
 
[[Team:Czech_Republic/Practices/GMO_survey|Read more about the survey...]]
 
[[Team:Czech_Republic/Practices/GMO_survey|Read more about the survey...]]
  
 
= [[Team:Czech_Republic/Safety|Safety]] =
 
= [[Team:Czech_Republic/Safety|Safety]] =
We discussed the safety issues of our technology throughout the whole duration of the project since we were aware of working with modified S. cerevisiae with the vision to use them in a clinical setting.
+
We did not only wholeheartedly followed standard lab safety techniques and complied with the national GMO laboratory regulations but also considered the broader safety assumptions of our technology. We discussed our idea with non-biological specialists as well as experts, which enabled us to improve the design and get a better grasp of the notion our project idea transmits.
 +
 
 
[[Team:Czech_Republic/Safety|Read more...]]
 
[[Team:Czech_Republic/Safety|Read more...]]
  
 
{{:Team:Czech_Republic/Template:Bottom}}
 
{{:Team:Czech_Republic/Template:Bottom}}

Latest revision as of 02:32, 19 September 2015

Human Practices

Outreach strategy

The IOD band presents a new approach to diagnostics and diagnostic engineering. So at Team Czech Republic we addressed engineers and medical experts to discuss the application and the novel engineering approach. In addition, Czech Republic teamed up with Chalmers Gothenburg to conduct a multinational survey on the public perception of Synthetic Biology.

Engineering

Czech Republic IODLecture.png

Since a lot of ideas behind IODs were built on the fundamentals of classical engineering, we wanted to present the concept of our project (from an engineering point-of-view) to young engineers and receive their feedback. We presented our project and synthetic biology in general at a lecture of the Introduction to Cybernetics class led by Prof. Ing. Miloš Schlegel CSc. After giving a 30-minute lecture on synthetic biology, we introduced our project. The response was surprisingly positive. Not only we were asked several to-the-point questions, but some of the students even showed a deeper interest in synthetic biology and individually contacted us after the talk.

Medicine

Czech Republic Interview introduction.png

In order to get a professional opinion on our project and to learn more about cancer and CTCs, we conducted an interview with two cancer specialists, Mgr. Pavel Pitule, Ph.D. and Mgr. Pavel Ostašov, Ph.D. The interview took place at the Biomedical Center of Faculty of Medicine in Pilsen. After introducing our project idea we discussed some particularly interesting points. This interview was not only helpful and enriching but also led to an agreement to collaborate with the Laboratory of Tumor Biology in the near future.


Key outputs

Most engineers are eager to see the immediate reaction of the system. Following the lectures such as the one for young engineers, we designed IODs as a fast diagnostic test. Also, the preliminary process is quick thanks to the clone-free preparation of required IODs.

Questions we received during the lecture from students and teachers concerning current cancer diagnostics, their price, and efficiency were afterwards discussed with cancer specialists in the interview.

Read more about the lecture...


Key outputs

We were recommended to use a color change instead of the agglutination as an indicator of CTCs' presence. As a result, a group of experiments from module 2 was changed to use GFP as an indicator of successful communication between IODs. The use of color indication in the further research proved advantageous (at least partially), and the use of color indicators will always be considered in later experiments.


A recommendation was made to test the agglutination on a slide. As a result, since we had the possibility to perform our experiments in microfluidics, we listened to this advice and used microfluidics as well as microscope slides in our agglutination experiments.

Read more about the interview...


Multinational general public vs. biologists GMO survey

Team Chalmers Gothenburg offered us to participate in their GMO survey. We asked 63 biologists and 50 respondents from the general public to fill in the survey. Same thing was done in Sweden. Thanks to this, we had the opportunity to compare GMO knowledge in Sweden and Czech Republic. Big thanks belongs to the team Chalmers Gothenburg for statistical evaluation of all data.

Key outputs

The positive attitude and further interest of many respondents delighted us.

However, although anticipated, the difference in opinions between biologists and the general public is disturbing. On that account, we are going to arrange more lectures about synthetic biology at our university and also for the general public in Czech Republic.

Read more about the survey...

Safety

We did not only wholeheartedly followed standard lab safety techniques and complied with the national GMO laboratory regulations but also considered the broader safety assumptions of our technology. We discussed our idea with non-biological specialists as well as experts, which enabled us to improve the design and get a better grasp of the notion our project idea transmits.

Read more...