Lab

The wet lab work we did in the course of this project was as diverse as it can possibly be. We worked with mammalian cell culture, did a lot of experiments and cloning with E. coli, developped and tested a microfluidics chip and, last but not least, we combined all these three parts together. We invested a lot of time into the generation of reliable data sets and never stopped looking for more experiments to do that would make it possible for us to achieve the most fruitful interaction with the modeling team, which in turn helped us a lot in setting a focus on critical points.

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Please take a look at our experimental Results to see how we achieved

• the characterization of 15 newly designed promoters controlled by LldR and Lactate
• the characterization of 2 newly designed fusion-promoters, combining repression by LldR and LacI
• the closer elucidation of the never characterized mechanism of action behind the (link)LldR-mediated repression of the LldR-operator-promoter
• the observation of (link)binding of bacteria to apoptotic mammalian cells
• the validation of a tight (link)AND gate

and many other things.

We are also happy to present to you the process of desinging a proper testing system of our final test setup in a microfluidic chip.

If you want to know more about the experiments we performed, please visit our Experiments page.

We are one of 71 teams that participated at this years Interlab Study, in which we measrued the fluorescence ot the three test constructs using FACS, as well as our plate reader.