Team:Lethbridge HS/Description

Project

How does our project work?

• Description

• What is nuclease? What is dextranase?

• What we are doing differently

• Extracellular Polymeric Substance Matrix

Overview

Without the bees planning dinner would be significantly more difficult. Bees pollinate 70 out of the top 100 food crops, which supply 90% of the world nutrition. It is apparent that bees are an integral part of the ecosystem and human life. However, bees have been in decline for about 30 years, but the rate of deaths have gone up in the past decade. In the United States, a startling 30% of bees are dying each year and this is due to a phenomenon called Colony Collapse Disorder (CCD) which is destroying productive bee colonies worldwide. One factor contributing to CCD is the parasitic mite, Varroa destructor. The parasite sucks the bees’ haemolymph (blood), and transmits RNA viruses, such as Deformed Winged Virus, which are detrimental to colony productivity. Current methods used to control V. destructor are inefficient and resistance is developing in treated populations. Using synthetic biology, we designed E.coli that produce the miticide oxalic acid in the bee gut. This method targets V. destructor by directly delivering oxalic acid into the mites, creating mite-proof bee populations.