Team:Lethbridge HS/Results
Results
What results did we get in the lab?
Results
Oxalic acid results:
| Oxalic Acid Concentration (mg/mL) | OD528 |
|---|---|
| 0.1 | 1.862 |
| 0.2 | 1.675 |
| 0.3 | 1.675 |
| 0.4 | 1.655 |
| 0.5 | 1.673 |
| 0.6 | 1.680 |
| 0.7 | 1.655 |
| 0.8 | 1.630 |
| 0.9 | 1.587 |
| 1.0 | 1.564 |
Bee trials:
| Treatment | Before | After |
|---|---|---|
| None | Somewhat lethargic. Drank when prompted. | Appeared tired, but otherwise unaffected. |
| 0.00% | Most lethargic, did not eat when offered | Wet and slow moving. |
| 0.05% | Very vigorous; ran around inside the tube. Fed. | Very energetic. Unaffected by treatment. |
| 0.2% | Very vigorous; ran around inside the tube. Fed. | Slightly less energetic than the 0.05% bee. |
The bees will be able to survive all of the oxalic acid that the E.coli can produce.
Mite trials:
| Control | Oxalic Acid | RFP | |
|---|---|---|---|
| Parafilm Pouch | Mites dead | Mites dead | Mites dead |
| Larvae 1 | Mites dead | Mites dead | Mites dead |
| Larvae 2 | Mites dead | Mites dead | Mites dead |
The mites did not survive any of the trials.
Antibiotics testing:
| Test (Antibiotics) | Result | After |
|---|---|---|
| AMP 1 | Positive | |
| AMP 2 | Positive | Wet and slow moving. |
| CAM 1 | Positive | |
| CAM 2 | Positive | |
| TET 1 | Positive | |
| TET 2 | Positive | |
| Control (water) | Positive |
The results we got for antibiotics testing show that biofilms can still be recultured even after being treated with antibiotics.
Testing with cleaners
| Cleaner | Results | After |
|---|---|---|
| Bleach | - | |
| Tilex | 3 | |
| Mr. Clean | - | |
| Fantastik | 1 | |
| Truly | 2 | |
| Windex | 1 | |
| Lysol | - | |
| GreenWorks | 1 |
