Team:Lethbridge HS/Results
Results
What results did we get in the lab?
Results
Oxalic acid results:
| Oxalic Acid Concentration (mg/mL) | OD528 |
|---|---|
| 0 | 1.7104 |
| 0.01 | 1.817 (only one trial) |
| 0.5 | 1.647 (only one trial) |
| 0.1 | 1.145125 |
| 0.15 | 1.6255 (only two trials) |
| 0.2 | 0.7021428571 |
| 0.25 | 1.5195 (only two trials) |
| 0.3 | 0.588375 |
| 0.35 | 1.696 (only one trial) |
| 0.4 | 0.7051 |
| 0.45 | 1.737 (only one trial) |
| 0.5 | 0.6271428571 |
| 0.55 | 1.666 (only one trial) |
| 0.6 | 0.6502222222 |
| 0.65 | 1.674 (only one trial) |
| 0.7 | 0.6052857143 |
| 0.75 | 1.597 (only one trial) |
| 0.8 | 0.7459 |
| 0.85 | 1.533 (only one trial) |
| 0.9 | 0.504 |
| 1.0 | 0.6849 |
| 1.4 | 0.789 (only two trials) |
| 1.5 | 0.983 (only one trial) |
| 1.6 | 0.8195 (only two trial) |
| 1.8 | 0.047 (only one trials) |
| 1.6 | 0.037 (only two trials) |
Bee trials:
| Treatment | Before | After |
|---|---|---|
| None | Somewhat lethargic. Drank when prompted. | Appeared tired, but otherwise unaffected. |
| 0.00% | Most lethargic, did not eat when offered | Wet and slow moving. |
| 0.05% | Very vigorous; ran around inside the tube. Fed. | Very energetic. Unaffected by treatment. |
| 0.2% | Very vigorous; ran around inside the tube. Fed. | Slightly less energetic than the 0.05% bee. |
The bees will be able to survive all of the oxalic acid that the E.coli can produce.
Mite trials:
| Control | Oxalic Acid | RFP | |
|---|---|---|---|
| Parafilm Pouch | Mites dead | Mites dead | Mites dead |
| Larvae 1 | Mites dead | Mites dead | Mites dead |
| Larvae 2 | Mites dead | Mites dead | Mites dead |
The mites did not survive any of the trials.
Antibiotics testing:
| Test (Antibiotics) | Result | After |
|---|---|---|
| AMP 1 | Positive | |
| AMP 2 | Positive | Wet and slow moving. |
| CAM 1 | Positive | |
| CAM 2 | Positive | |
| TET 1 | Positive | |
| TET 2 | Positive | |
| Control (water) | Positive |
The results we got for antibiotics testing show that biofilms can still be recultured even after being treated with antibiotics.
