Difference between revisions of "Team:NRP-UEA-Norwich/Results/Probiotic"
Line 327: | Line 327: | ||
<h2 class="title1">Making constructs to express putative acyl-transferases in plant chloroplasts</h2> | <h2 class="title1">Making constructs to express putative acyl-transferases in plant chloroplasts</h2> | ||
− | <h4 class="title2">AIM</h4> | + | <h4 class="title2">AIM:</h4> |
<p class="space10">The aim of our prebiotic is to produce acylated/butrylated starch in plants. Methods to chemically acylate starch purified from plants already exist, but as they use strong chemicals and require heating they are not environmentally friendly. Using various acyltransferases, we hope to acylate starch in plants. We’ll be using a model plant, <i>Nicotinia benthamiana</i>, for initial tests because we can get results within a few days. Later on we’d aim to make transgenic plants in a species makes a lot of starch such as maize (corn), potatoes or wheat.</p> | <p class="space10">The aim of our prebiotic is to produce acylated/butrylated starch in plants. Methods to chemically acylate starch purified from plants already exist, but as they use strong chemicals and require heating they are not environmentally friendly. Using various acyltransferases, we hope to acylate starch in plants. We’ll be using a model plant, <i>Nicotinia benthamiana</i>, for initial tests because we can get results within a few days. Later on we’d aim to make transgenic plants in a species makes a lot of starch such as maize (corn), potatoes or wheat.</p> | ||
Line 337: | Line 337: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-md-12"> | <div class="col-md-12"> | ||
− | <p class="space30"> | + | <h4 class="title2">METHOD:</h4> |
+ | <p class="space30">We used Golden Gate Cloning and the Plant Standard Syntax described in RFC 106 and Patron et al (2015) to make our constructs. We used 35s promoter from Cauliflower Mosiac Virus (BBa_K1467101), to drive constitutive expression. We made a chloroplast transit peptide (New part - BBa_K1618028) in an N-terminal translation fusion with the acyl transferases for the protein to reach the chloroplast, where starch is produced. | ||
+ | </p> | ||
<p class="space30">This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p> | <p class="space30">This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p> |
Revision as of 15:55, 17 September 2015
RESULTS - PROBIOTIC
Title Here
Maybe a Date Here?
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be.
Figure 1: A model structure of glycogen with acyl groups added.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Title Here2
Maybe a Date Here?
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Title Here3
Maybe a Date Here?
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Title Here4
Maybe a Date Here?
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Title Here 5
Maybe a Date Here?
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Title Here 6
Maybe a Date Here?
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Making constructs to express putative acyl-transferases in plant chloroplasts
AIM:
The aim of our prebiotic is to produce acylated/butrylated starch in plants. Methods to chemically acylate starch purified from plants already exist, but as they use strong chemicals and require heating they are not environmentally friendly. Using various acyltransferases, we hope to acylate starch in plants. We’ll be using a model plant, Nicotinia benthamiana, for initial tests because we can get results within a few days. Later on we’d aim to make transgenic plants in a species makes a lot of starch such as maize (corn), potatoes or wheat.
METHOD:
We used Golden Gate Cloning and the Plant Standard Syntax described in RFC 106 and Patron et al (2015) to make our constructs. We used 35s promoter from Cauliflower Mosiac Virus (BBa_K1467101), to drive constitutive expression. We made a chloroplast transit peptide (New part - BBa_K1618028) in an N-terminal translation fusion with the acyl transferases for the protein to reach the chloroplast, where starch is produced.
This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Title Here 8
Maybe a Date Here?
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
FLAV
Maybe a Date Here?
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.
Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.