Difference between revisions of "Team:NRP-UEA-Norwich/Results/Probiotic"

Line 114: Line 114:
 
                       <li role="presentation" class="aqua scroll-link" data-id="top"><a href="#setting2" aria-controls="content2" role="tab" data-toggle="tab"><span>- Title of page</span></a></li>
 
                       <li role="presentation" class="aqua scroll-link" data-id="top"><a href="#setting2" aria-controls="content2" role="tab" data-toggle="tab"><span>- Title of page</span></a></li>
 
                       <li role="presentation" class="aqua scroll-link" data-id="top"><a href="#setting3" aria-controls="content3" role="tab" data-toggle="tab"><span>- Title of page</span></a></li>
 
                       <li role="presentation" class="aqua scroll-link" data-id="top"><a href="#setting3" aria-controls="content3" role="tab" data-toggle="tab"><span>- Title of page</span></a></li>
                       <li role="presentation" class="aqua scroll-link" data-id="top"><a href="#setting4" aria-controls="content4" role="tab" data-toggle="tab"><span>- Cloning of Plant Constructs</span></a></li>
+
                       <li role="presentation" class="aqua scroll-link" data-id="top"><a href="#setting4" aria-controls="content4" role="tab" data-toggle="tab"><span>- Longish title of thing</span></a></li>
 
                       <li role="presentation" class="aqua scroll-link space50" data-id="top"><a href="#setting5" aria-controls="content4" role="tab" data-toggle="tab"><span>- Title of page</span></a></li>
 
                       <li role="presentation" class="aqua scroll-link space50" data-id="top"><a href="#setting5" aria-controls="content4" role="tab" data-toggle="tab"><span>- Title of page</span></a></li>
 
<li role="presentation" class="aqua scroll-link" data-id="top"><a href="#setting6" aria-controls="content4" role="tab" data-toggle="tab"><span>- Title of page</span></a></li>
 
<li role="presentation" class="aqua scroll-link" data-id="top"><a href="#setting6" aria-controls="content4" role="tab" data-toggle="tab"><span>- Title of page</span></a></li>
Line 326: Line 326:
 
                       <div class="col-md-12 space50">
 
                       <div class="col-md-12 space50">
  
                         <h2 class="title1">Making constructs to express putative acyl-transferases in plant chloroplasts</h2>
+
                         <h2 class="title1">Title Here2</h2>
                         <h4 class="title2">AIM:</h4>
+
                         <p class="fsize20">Maybe a Date Here?</p>
  
                         <p class="space10">The aim of our prebiotic is to produce acylated/butrylated starch in plants. Methods to chemically acylate starch purified from plants already exist, but as they use strong chemicals and require heating they are not environmentally friendly. Using various acyltransferases, we hope to acylate starch in plants. We’ll be using a model plant, <i>Nicotinia benthamiana</i>, for initial tests because we can get results within  a few days. Later on we’d aim to make transgenic plants in a species makes a lot of starch such as maize (corn), potatoes or wheat.</p>
+
                         <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
 +
 
 +
                        <p class="space20">This new property means that silicon-based light detectors identify spin, so more information can be.</p>
 
                       </div>
 
                       </div>
  
                        
+
                       <div class="col-md-6 space80">
 +
                        <img src="image/Section-1-Photo.png" alt="..." class="img-responsive mautomargin">
 +
                      </div>
 
                     </div>   
 
                     </div>   
  
 
                     <div class="row">   
 
                     <div class="row">   
 
                         <div class="col-md-12">
 
                         <div class="col-md-12">
<h4 class="title2">METHOD:</h4>
+
                             <p class="space30">Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
                             <p class="space10">We used Golden Gate Cloning  and the Plant Standard Syntax<sub><a data-id="ref" class="scroll-link" style = "color: #002bb8;">1</a></sub> to make our constructs. We used 35s promoter from Cauliflower Mosiac Virus (BBa_K1467101), to drive constitutive expression.  We made a chloroplast transit peptide (New part - BBa_K1618028) in an N-terminal translation fusion with the acyl transferases for the protein to reach the chloroplast, where starch is produced.
+
</p>
+
 
+
<img src="https://static.igem.org/mediawiki/2015/f/f5/NRP-UEA-Norwich-results-leda1.png" href="https://static.igem.org/mediawiki/2015/f/f5/NRP-UEA-Norwich-results-leda1.png" alt="..." class="img-responsive mautomargin fancybox" style="cursor: pointer;">
+
                            <p class="space20">In order to confirm that the transit peptide was functional we made a second set of constructs that had a fluorescent reporter in a C-terminal translational fusion.</p>
+
 
+
<img src="https://static.igem.org/mediawiki/2015/a/a9/NRP-UEA-Norwich-results-leda2.png" href="https://static.igem.org/mediawiki/2015/a/a9/NRP-UEA-Norwich-results-leda2.png" alt="..." class="img-responsive mautomargin fancybox" style="cursor: pointer;">
+
<p class="space20">To deliver the constructs to plants we needed to use Agrobacterium tumefaciens as a shuttle chassis. This meant parts needed to be assembled into a binary vector with origins of replication for both E.coli and A. tumefaciens. In order to submit the parts to the registry we also assembled parts into the MoCloFlipper pSB1C3 that accepts Golden Gate Parts into the pSB1C3 backbone.</p>
+
 
+
<p>All cloning was done according to the Golden Gate one-step Digestion-Ligation Protocol provided on our protocols page <a href="https://2015.igem.org/Team:NRP-UEA-Norwich/Protocols#Ones-Step Golden Gate Digestion Ligation Protocol" style = "color: #002bb8;">here</a>.</p>
+
 
+
<h4 class="title2">Results:</h4>
+
  
 
                             <p class="space30">This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>
 
                             <p class="space30">This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.</p>

Revision as of 17:04, 17 September 2015

House of Carbs

RESULTS - PROBIOTIC

Title Here

Maybe a Date Here?

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be.

...

Figure 1: A model structure of glycogen with acyl groups added.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Title Here2

Maybe a Date Here?

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be.

...

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Title Here3

Maybe a Date Here?

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be.

...

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Title Here4

Maybe a Date Here?

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be.

...

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Title Here 5

Maybe a Date Here?

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be.

...

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Title Here 6

Maybe a Date Here?

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be.

...

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Title Here2

Maybe a Date Here?

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be.

...

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

References

1. Patron et al (2015) Standards for Plant Synthetic Biology: A Common Syntax for Exchange of DNA Parts doi: 10.1111/nph.13532/full

Title Here 8

Maybe a Date Here?

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be.

...

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

FLAV

Maybe a Date Here?

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be.

...

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

This new property means that silicon-based light detectors identify spin, so more information can be. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin. Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.Currently, copper wires transfer information in a computer; the process is slowed down as the wires heat up. "Photonic" and "spintronic" computing is the principle of transferring information by light or electron spin.

MANY THANKS TO OUR SPONSORS

Useful Links

Contact Us

  • nrpuea.igem2015@gmail.com

  • Norwich Research Park,
  • Colney,
  • Norwich, NR4 7UH, UK.

We are the NRP-UEA-Norwich 2015 iGEM Team.

Designed and developed by the NRP-UEA-Norwich iGEM Team