Difference between revisions of "Team:Northeastern Boston/notebook"

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<p>Northeastern has decided to use Protocols.io to host all of its iGEM protocols. Protocols.io is an online open source wet lab repository. Researchers can upload their experimental protocol, share it others, build in functionality (like timers), and "fork" other researchers' protocols (adapting them for their particular situation).</p>
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<p>Northeastern has decided to use <a href="https://www.protocols.io/" target="_blank">Protocols.io</a> to host all of its iGEM protocols. Protocols.io is an online open source wet lab repository. Researchers can upload their experimental protocol, share it others, build in functionality (like timers), and "fork" other researchers' protocols (adapting them for their particular situation).</p>
  
 
<p>We think this is worth breaking the <a href="https://2015.igem.org/Wiki_Requirements" target="_blank">"No iFrames"</a> rule. The benefit of teams being able to create a sharable protocol, with easy reference and modifiability, outweigh the fear of "cheating" from iGEM teams altering the externally hosted content (in part because altering lab protocols is relatively benign). Other teams could, with the Protocols.io app, exactly replicate the protocol that we followed, for example, when we transformed <a href="https://www.protocols.io/view/Glass-Bead-Transformation-of-Chlamydomonas-dtn6md" target="_blank"><i>C. reinhardtii.</i></a></p>
 
<p>We think this is worth breaking the <a href="https://2015.igem.org/Wiki_Requirements" target="_blank">"No iFrames"</a> rule. The benefit of teams being able to create a sharable protocol, with easy reference and modifiability, outweigh the fear of "cheating" from iGEM teams altering the externally hosted content (in part because altering lab protocols is relatively benign). Other teams could, with the Protocols.io app, exactly replicate the protocol that we followed, for example, when we transformed <a href="https://www.protocols.io/view/Glass-Bead-Transformation-of-Chlamydomonas-dtn6md" target="_blank"><i>C. reinhardtii.</i></a></p>

Revision as of 22:22, 18 September 2015

DNA

HiFi DNA Assembly
Plasmid Miniprep
Gel Electrophoresis
PCR with Phusion
Gel DNA Recovery
T4 Ligation
Restriction Digest

Escherichia coli

LB Agar Plates
E. coli Heat Shock

Chlamydomonas reinhardtii

TAP Agar Plates
Glass Bead Transformation

Protocols.io