Member’s Attributions
ZHANG Yihao is the team leader of Peking iGEM 2015. He participated in conceiving the project, took charge of troubleshooting, and managed the experiments, guaranteeing the normal operation of the lab.
WEI Weijia was committed to ameliorate the paired dCas9 (PC) reporter system, namely, to enhance the sensitivity and robustness. Early in the project, he was also responsible for information gathering, experiment design, and lab management
LI Hang was responsible for designing and manufacturing the portable electronic device that convert optical signal into analogue electrical signal.
CHENG Sida was in charge of Human Practice and also worked in the wet lab to construct and prepare plasmids for gRNA in vitro transcription.
LI Jiaofeng worked as the financial manager of the team. She was also responsible for plasmid construction and submission.
LI Xiang was in charge of building M.tuberculosis-sensing system using Molecule Beacon. He was also engaged in the protein purification of dCas9 fusion proteins.
LING Pingping was in charge of measuring the luminescence intensity of PC reporter system. She analyzed the entire experimental data.
LIU Dongming helped to complete the plasmid construction of sgRNA and was in part responsible for human practice.
LIU Wenchao was in charge of the construction of CRISPR gRNA as well as RNA scaffold in the beginning. Later she was working on the purification of dCas9 fusion protein.
WANG Beining took the task of cloning dCas9-luciferase. She was also responsible for the testing of PC reporter system.
WANG Yu participated in plasmid construction, protein purification, and wiki building. She was also the designer of our PowerPoint.
XU Luze was in charge of Modeling. His main task was to computationally search for the suitable guide sequences. Also, he was the lab manager for the normal operation of our lab.
YE Yixuan was responsible for human practice and part of the mathematical modeling.
Zhang Xiaohan was in charge of Conference of China iGEMer’s Committee and also helped in human practice and poster design.
Acknowledgement
Peking iGEM 2015 team gratefully acknowledges the following institutes:
- School of Life Sciences, Peking University
- Office of Educational Administration, Peking University
- Center for Quantitative Biology, Peking University
- School of Physics, Peking University
- School of Basic Medical Sciences, Peking University
- Institute of Microbiology, Chinese Academy of Sciences
- Beijing Research Institute for Tuberculosis Control
We thank the following professors for supporting our project with equipments and chemicals :
- Prof. OUYANG Qi
- Prof. LOU Chubo
- Prof. LAI Luhua
- Prof. HE Xinqiang
- Prof. XU Chongren
Lab support
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We would like to thank:
Dr. ZHAO Huiwei for providing us with the plasmid of dCas9 and the linker(GGGGS*2) for fusion proteins; -
Professor LAI Luhua for providing us with the pET21a plasmid; -
Dr. HOU Junran for providing us with the plasmid carrying FRB and FKBP proteins; -
ZHANG Haoqian for providing us with the pSB1A3 plasmid with BsaI sites removed; -
Graduate LIU Ruicun from USTC for giving us the plasmid WJ024X containing DHFR; -
Dr. ZHAO Xuejin for giving us the plasmid containing gRNA Generator, technical support, and equipment support; -
Dr. LIU Cuihua for providing us the isolated genomic of M.tuberculosis.
Wiki support
Our instructor, ZHANG Haoqian, gave us many ideas about the structure of wiki and the polishment of language.
Presentation coaching
- Professor OUYANG Qi and Professor LOU Chunbo coached us for the presentation.
- Professor LAI Luhua, Professor LI Fangting and Professor RAO Yi participated in our presentation rehearsal and made suggestions for revisions.
Human practices support
- Doctor LI Bo from Beijing research institute for tuberculosis control was an invaluable resource for informing us on all aspects of TB facts.
- Director DING Beichuan of Central Laboratory in Beijing research institute for tuberculosis control showed us around the advanced TB detecting laboratory and gave advice for our detecting system.
- Director WANG Suming from Beijing research institute for tuberculosis control accepted our interview and gave advice for our project.
- Doctor XIE Guangkuan from the Institute of Medical Humanities of Peking University accepted our interview and advised on the ethics discussion.
- Director XIA Kebiao from Infectious Disease Department in Zhenghe County Hospital and Director LV Tingrong from Zhenghe Epidemic Prevention Station provided us with the epidemic situation of TB in grass-roots units.
- Zhenghe No.1 Middle School of Fujian Province provided us a chance for advertising the basic knowledge of TB control and synthetic biology to the high school students.
- ZHAO Mingyue, a freshman of Heilongjiang University, gave us much help in questionnaire survey, handing out propagandistic handbooks and maintaining the lab operation.
Fundraising help and advice
- BluePHA® and Synbio Tech® supported the CCiC (.Conference of China iGEMer Committee) as sponsors.
- Our project is funded by School of Life Sciences, Peking University.
Last but not least
- We would like to thank all the people who came to our progress presentations and gave useful feedback and input.
- We would like to thank all the 34 iGEM teams for participanting the CCiC and the volunteers for providing indispensable help. They really gave us lots of inspiration and suggestions.