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| | <h3 class="classic-title"><span>Favorite Parts</span></h3> | | <h3 class="classic-title"><span>Favorite Parts</span></h3> |
| | <img src="https://static.igem.org/mediawiki/2015/0/08/Peking-team-about-01.jpg" style="width:100%"> | | <img src="https://static.igem.org/mediawiki/2015/0/08/Peking-team-about-01.jpg" style="width:100%"> |
| − | <p>At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores et quas molestias excepturi sint occaecati cupiditate non provident, similique sunt in culpa qui officia deserunt mollitia animi, id est laborum et dolorum fuga. Et harum quidem rerum facilis est et expedita distinctio. Nam libero tempore, cum soluta nobis est eligendi optio cumque nihil impedit quo minus id quod maxime placeat facere possimus, omnis voluptas assumenda est, omnis dolor repellendus. Temporibus autem quibusdam et aut officiis debitis aut rerum necessitatibus saepe eveniet ut et voluptates repudiandae sint et molestiae non recusandae. Itaque earum rerum hic tenetur a sapiente delectus, ut aut reiciendis voluptatibus maiores alias consequatur aut perferendis doloribus asperiores repellat.</p> | + | <p>Nluc-dCas9 fusion protein</p> |
| − | </div>
| + | <p>(BBa_K1689010)</p> |
| − | <!--divider-->
| + | <p>Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Cluc-dCas9 fusion protein ( BBa_K1689009) and sgRNAs, our paired dCas9 reporter system were constructed.</p> |
| − | <div class="hr5" style="margin-top:25px;margin-bottom:45px"></div>
| + | <p>Cluc-dCas9 fusion protein</p> |
| | + | <p>( BBa_K1689009)</p> |
| | + | <p>Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Nluc-dCas9 fusion protein ( BBa_K1689010) and sgRNAs, our paired dCas9 reporter system were constructed.</p> |
| | + | <p>sgRNA generator</p> |
| | + | <p>(BBa_K1689000)</p> |
| | + | <p> sgRNA generator is used for generating sgRNA which recognizes targeted DNA, then guiding dCas9 to it. |
| | + | </p> |
| | + | |
| | + | </div> |
| | | | |
| − | <div id="Part list">
| + | <h3 class="classic-title"><span>Part List</span></h3> |
| − | <h3 class="classic-title"><span>Parts List</span></h3> | + | <h4>Parts for paired dCas9 reporter system</h4> |
| − | <table class="table-striped table-hover col-md-12" style="font-family: 'Open Sans', sans-serif; font-size: 13px;padding:5px;"> | + | |
| − | <tr>
| + | |
| − | <th style="padding-right:8px">
| + | |
| − | </th><th style="padding-right:8px">Name
| + | |
| − | </th><th style="padding-right:8px">Description
| + | |
| − | </th><th style="padding-right:8px">Length
| + | |
| − | </th>
| + | |
| − | </tr>
| + | |
| − |
| + | |
| − | <tr>
| + | |
| − | <td style="padding-right:8px"> A/U
| + | |
| − | </td><td><a href="" style="color:#00afd1">xxx</a>
| + | |
| − | </td><td>At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores
| + | |
| − | </td><td>1234
| + | |
| − | </td>
| + | |
| − | </tr>
| + | |
| | | | |
| − | <tr>
| + | <p>Nluc416-FRB(BBa_K1689003), FKBP-Cluc398(BBa_K1689005), Nluc398-FRB(BBa_K1689004) and FKBP-Cluc394(BBa_K1689006) are used to validate the reconsitituation activity of split luciferase.</p> |
| − | <td> A/U
| + | <p>Nluc-dCas9 (BBa_K1689010), Cluc-dCas9 ( BBa_K1689009), dCas9-Nluc(BBa_K1689008) and dCas9-Cluc (BBa_K1689007) fusion proteins are used for constructing paired dCas9 reporter system.</p> |
| − | </td><td><a href=""style="color:#00afd1">xxx</a>
| + | <p>delta alpha-dCas9(BBa_K1689011), dCas9-Nlact(BBa_K1689012), Nlact-dCas9(BBa_K1689013), dCas9-Clact(BBa_K1689014), F[1,2]-dCas9(BBa_K1689015), dCas9- F[1,2](BBa_K1689016), F3-dCas9(BBa_K1689017), dCas9- F3(BBa_K1689018), dCas9-Δα(BBa_K1689019), delta omega-dCas9(BBa_K1689020) are used for further development of dectection.</p> |
| − | </td><td>At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores
| + | <h4>Parts for Molecular Beacon</h4> |
| − | </td><td>1234
| + | <p>STV-Nluc(BBa_K1689001) and Cluc-STV(BBa_K1689002) are used for binding to molecular beocon.</p> |
| − | </td>
| + | <h4>Parts for sgRNA generation</h4> |
| − | </tr>
| + | <p>sgRNA generator(BBa_K1689000) is used for generating sgRNA</p> |
| | + | </div> |
| | | | |
| − | <tr>
| |
| − | <td> A/U
| |
| − | </td><td><a href=""style="color:#00afd1">xxx</a>
| |
| − | </td><td>At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores
| |
| − | </td><td>1234
| |
| − | </td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
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| − | <td> A/U
| |
| − | </td><td><a href=""style="color:#00afd1">xxx</a>
| |
| − | </td><td>At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores
| |
| − | </td><td>1234
| |
| − | </td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
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| − | <td> A/U
| |
| − | </td><td><a href=""style="color:#00afd1">xxx</a>
| |
| − | </td><td>At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores
| |
| − | </td><td>1234
| |
| − | </td>
| |
| − | </tr>
| |
| − |
| |
| − | <tr>
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| − | <td> A/U
| |
| − | </td><td><a href=""style="color:#00afd1">xxx</a>
| |
| − | </td><td>At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores
| |
| − | </td><td>1234
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr>
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| − | <td> A/U
| |
| − | </td><td><a href=""style="color:#00afd1">xxx</a>
| |
| − | </td><td>At vero eos et accusamus et iusto odio dignissimos ducimus qui blanditiis praesentium voluptatum deleniti atque corrupti quos dolores
| |
| − | </td><td>1234
| |
| − | </td>
| |
| − | </tr>
| |
| − | </table>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| | </div> | | </div> |
| | + | <!--divider--> |
| | + | |
| | | | |
| | <!--FOOTER************FOOTER*******************FOOTER***************FOOTER************--> | | <!--FOOTER************FOOTER*******************FOOTER***************FOOTER************--> |
