Favorite Parts
Nluc-dCas9 fusion protein
(BBa_K1689010)
Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Cluc-dCas9 fusion protein ( BBa_K1689009) and sgRNAs, our paired dCas9 reporter system were constructed.
Cluc-dCas9 fusion protein
( BBa_K1689009)
Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Nluc-dCas9 fusion protein ( BBa_K1689010) and sgRNAs, our paired dCas9 reporter system were constructed.
sgRNA generator
(BBa_K1689000)
sgRNA generator is used for generating sgRNA which recognizes targeted DNA, then guiding dCas9 to it.
Part List
Parts for paired dCas9 reporter system
Nluc416-FRB(BBa_K1689003), FKBP-Cluc398(BBa_K1689005), Nluc398-FRB(BBa_K1689004) and FKBP-Cluc394(BBa_K1689006) are used to validate the reconsitituation activity of split luciferase.
Nluc-dCas9 (BBa_K1689010), Cluc-dCas9 ( BBa_K1689009), dCas9-Nluc(BBa_K1689008) and dCas9-Cluc (BBa_K1689007) fusion proteins are used for constructing paired dCas9 reporter system.
Δα-dCas9(BBa_K1689011), dCas9-Nlact(BBa_K1689012), Nlact-dCas9(BBa_K1689013), dCas9-Clact(BBa_K1689014), F[1,2]-dCas9(BBa_K1689015), dCas9- F[1,2](BBa_K1689016), F3-dCas9(BBa_K1689017), dCas9- F3(BBa_K1689018), dCas9-Δα(BBa_K1689019), delta omega-dCas9(BBa_K1689020) are used for further development of dectection.
Parts for Molecular Beacon
STV-Nluc(BBa_K1689001) and Cluc-STV(BBa_K1689002) are used for binding to molecular beocon.
Parts for sgRNA generation
sgRNA generator(BBa_K1689000) is used for generating sgRNA