Difference between revisions of "Team:Peking/Parts"

 
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<html lang="en">
 
<html lang="en">
 
<head>
 
<head>
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   <meta charset="utf-8">
 
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   <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1">
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    <!-- Page Description and Author -->
 
 
   <meta name="Peking-iGEM-2015" content="iGEM - Peking iGEM 2015">
 
   <meta name="Peking-iGEM-2015" content="iGEM - Peking iGEM 2015">
−
 
 
 
   <!--add  ?action=raw&amp;ctype=text/css    when using Css-->
 
   <!--add  ?action=raw&amp;ctype=text/css    when using Css-->
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  <!-- Bootstrap CSS V  -->
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   <link rel="stylesheet" href="https://2015.igem.org/Template:Peking/new?action=raw&amp;ctype=text/css" type="text/css" media="screen">
−
   <link rel="stylesheet" href="https://2015.igem.org/Template:Peking/CSS?action=raw&amp;ctype=text/css" type="text/css" media="screen">
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   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/Style/new?action=raw&amp;ctype=text/css" media="screen">
−
  <!-- Font Awesome CSS V -->
+
   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/Responsive/new?action=raw&amp;ctype=text/css" media="screen">
−
  <!--<link rel="stylesheet" href="https://2015.igem.org/Template:Peking/CSS/Font?action=raw&amp;ctype=text/css" type="text/css" media="screen">-->
+
   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/Animate/new?action=raw&amp;ctype=text/css" media="screen">
−
  <!-- Margo CSS Styles  V-->
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   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/Blue/new?action=raw&amp;ctype=text/css " title="blue" media="screen" />
−
   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/CSS/Style?action=raw&amp;ctype=text/css" media="screen">
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   <style type="text/css">/*get rid of the annoying iGEM style*/
−
  <!-- Responsive CSS Styles  -->
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         #top_menu_inside, #top_menu_inside:before, #top_menu_inside:after { -webkit-box-sizing: content-box; -moz-box-sizing: border-box; box-sizing: content-box;}
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   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/CSS/Responsive?action=raw&amp;ctype=text/css" media="screen">
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        #top_menu_under{font-size: 12px;}
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  <!-- Css3 Transitions Styles  -->
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        #top_menu_14{font-size: 12px; height:18px}
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   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/CSS/Animate?action=raw&amp;ctype=text/css" media="screen">
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        #top_menu_inside li{font-size: 12px;}
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  <!-- Color CSS Styles  -->
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        #user_item{font-size: 12px;}
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   <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Peking/Blue?action=raw&amp;ctype=text/css " title="blue" media="screen" />
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      #content{padding:0;border:0;width:100%;}
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       .firstHeading{visibility:hidden;}
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   <style type="text/css">
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       h2{border-bottom:none;padding-bottom:.5em;}     
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      /*get rid of the annoying iGEM style*/
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      .carousel-control.left, .carousel-control.right{background-image: none}
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         #content{
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     /* to style the sidebar*/
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            padding:0;
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            border:0;
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            width:100%;
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        }
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       .firstHeading{
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        visibility:hidden;
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       h2{
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        padding-bottom:.5em;
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        .carousel-control.left, .carousel-control.right {
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          background-image: none
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     /* to style the team page*/
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        .individual-picture{
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        max-width:200px;margin-top:5px; margin-bottom:10px;
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         @media (min-width:1024px){
 
         @media (min-width:1024px){
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       #sidebar1{position:relative;top:120px;}
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       #sidebar1{position:relative;top:120px;max-width:200px;}
 
       #sidebar2{display:none;}
 
       #sidebar2{display:none;}
 
       #nav-top{height:88px;}
 
       #nav-top{height:88px;}
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     }
 
     }
 
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       @media (max-width: 1023px){
 
       #sidebar1{display:none;}
 
       #sidebar1{display:none;}
 
       #sidebar2{display:block;}
 
       #sidebar2{display:block;}
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    }
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    .back-to-top{z-index:9999;}
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      ul.dropdown{margin-left: 0px; margin-top: 0px;}
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    .classic-title span {
 +
    position: absolute;
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    margin-top: -30px;
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    border-bottom-color: #00afd1;
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}
 +
.page-banner{
 +
  background-color:#e0e0e0;margin-top:1%;margin-bottom:20px;padding:20px;padding-top:40px;
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}
  
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    }
 
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     </style>
 
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</head>
  
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  <!--[if IE 8]><script src="http://html5shiv.googlecode.com/svn/trunk/html5.js"></script><![endif]-->
 
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</head>
 
 
<body>
 
<body>
 
   <!-- Container -->
 
   <!-- Container -->
 
   <div id="container">
 
   <div id="container">
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    <!-- Start Header -->
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    <!-- Start Header -->
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    <div class="hidden-header"></div>
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     <header class="clearfix">
 
     <header class="clearfix">
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         <!-- Start  Logo & Naviagtion  -->
 
         <!-- Start  Logo & Naviagtion  -->
 
             <div class="navbar navbar-default navbar-top">
 
             <div class="navbar navbar-default navbar-top">
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                     <div class="navbar-header" >
 
                     <div class="navbar-header" >
 
                         <!-- Stat Toggle Nav Link For Mobiles -->
 
                         <!-- Stat Toggle Nav Link For Mobiles -->
−
                         <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-collapse" style="marginn-top:20px">
+
                         <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-collapse" style="margin-top:40px">
 
                             <i class="fa"><img  style="margin-left:0;height:30px;"src="https://static.igem.org/mediawiki/2015/4/45/Peking-Hambergur-1.png" ></i>
 
                             <i class="fa"><img  style="margin-left:0;height:30px;"src="https://static.igem.org/mediawiki/2015/4/45/Peking-Hambergur-1.png" ></i>
 
                         </button>
 
                         </button>
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                     </div>
 
                     </div>
 
                     <div class="navbar-collapse collapse"  style="padding-top:5px;padding-bottom:0">
 
                     <div class="navbar-collapse collapse"  style="padding-top:5px;padding-bottom:0">
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                         <!-- Start Navigation List -->
 
                         <!-- Start Navigation List -->
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                         <ul class="nav navbar-nav navbar-right " id="nav-top" style="padding-bottom:15px;padding-top:10px">
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                         <ul class="nav navbar-nav navbar-right " style="padding-bottom:15px;padding-top:10px">
 
                             <li>
 
                             <li>
−
                                 <a href="https://2015.igem.org/Team:Peking">Home</a>
+
                                 <a href="https://2015.igem.org/Team:Peking/JudgingCriteria" class="active">Achievements</a>
−
                            </li>
+
−
              <li>
+
−
                                <a class="active" href="https://2015.igem.org/Team:Peking/JudgingCriteria">Achievements</a>
+
 
                                 <ul class="dropdown">
 
                                 <ul class="dropdown">
 
                                     <li><a href="https://2015.igem.org/Team:Peking/JudgingCriteria">Judging Criteria</a>
 
                                     <li><a href="https://2015.igem.org/Team:Peking/JudgingCriteria">Judging Criteria</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a class="active" href="#">Parts</a>
+
                                     <li><a class="active" href="https://2015.igem.org/Team:Peking/Parts">Parts</a>
 
                                     </li>
 
                                     </li>
 
                                     <li><a href="https://2015.igem.org/Team:Peking/Collaborations">Collaborations</a>
 
                                     <li><a href="https://2015.igem.org/Team:Peking/Collaborations">Collaborations</a>
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                                     <li><a href="https://2015.igem.org/Team:Peking/Design">Overview</a>
 
                                     <li><a href="https://2015.igem.org/Team:Peking/Design">Overview</a>
 
                                     </li>
 
                                     </li>
−
<li><a href="#">Background</a>
+
<li><a href="https://2015.igem.org/Team:Peking/Design/PC_Reporter">Paired<span style="text-transform:lowercase"> d</span>Cas9 Reporter</a>                                   
 +
</li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Design/Isothermal">Iso-<span style="text-transform:lowercase">t</span>hermal Amplification</a></li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Device">Hardware</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a href="#">CRIPSR</a>
+
                                     <li><a href="https://2015.igem.org/Team:Peking/Design/Speculation">Speculation</a>
−
                                    </li>
+
−
                                    <li><a href="#">Isothermal PCR</a>
+
−
                                    </li>
+
−
                                    <li><a href="#">B. subtillis</a>
+
−
                                    </li>
+
−
                                    <li><a href="#">Device</a>
+
 
                                     </li>
 
                                     </li>
 
                                 </ul>
 
                                 </ul>
 
                             </li>
 
                             </li>
 
                             <li>
 
                             <li>
−
                                 <a href="https://2015.igem.org/Team:Peking/Modeling">Modelling</a>
+
                                 <a href="https://2015.igem.org/Team:Peking/Modeling">Modeling</a>
 
                                 <ul class="dropdown">
 
                                 <ul class="dropdown">
−
                                     <li><a href="#">Link 1</a>
+
                                     <li><a href="https://2015.igem.org/Team:Peking/Modeling">Array Design</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a href="#">Link 2</a>
+
                                     <li><a href="https://2015.igem.org/Team:Peking/Modeling/Analysis">Analysis algorithm</a>
 
                                     </li>
 
                                     </li>
 
                                 </ul>
 
                                 </ul>
 
                             </li>
 
                             </li>
 
                             <li>
 
                             <li>
−
                                 <a href="https://2015.igem.org/Team:Peking/Practices">Human Practice</a>
+
                                 <a href="https://2015.igem.org/Team:Peking/Practices">Practices</a>
 
                                 <ul class="dropdown">
 
                                 <ul class="dropdown">
−
                                     <li><a href="#">Link 1</a>
+
                                     <li><a href="https://2015.igem.org/Team:Peking/Practices">Overview</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a href="#">Link 2</a>
+
                                     <li><a href="https://2015.igem.org/Team:Peking/Practices/Background">TB Facts</a>
 +
                                    </li>
 +
                                    <li><a  href="https://2015.igem.org/Team:Peking/Practices/Consultation">Consultation and Interview</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a href="#">Link 3</a>
+
                                     <li><a href="https://2015.igem.org/Team:Peking/Practices/Engagement">Public Engagement</a>
 
                                     </li>
 
                                     </li>
−
                                     <li><a href="#">Link 4</a>
+
                                     <li><a href="https://2015.igem.org/Team:Peking/Practices/Outreach">Ethics and Economics Issues</a>
 
                                     </li>
 
                                     </li>
 
                                 </ul>
 
                                 </ul>
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     <!-- Start Page Banner -->
 
     <!-- Start Page Banner -->
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     <div class="page-banner" style="background-color:#e0e0e0;margin-top:1%;margin-bottom:20px;">  
+
     <div class="page-banner">  
 
       <div class="container">
 
       <div class="container">
 
         <div class="row">
 
         <div class="row">
 
           <div class="col-md-6">
 
           <div class="col-md-6">
−
             <h2><b>Parts</b></h2>
+
             <h2 style="font-size:20px; margin-bottom:5px; padding-bottom:0" >P<span style="text-transform:lowercase">arts</span></h2>
−
             <p>Each part give us a new experience.</p>
+
             <p style="margin-top:0px;font-size:14px" id="Overview">Each part gives us a new experience.</p>
 
           </div>
 
           </div>
 
           <div class="col-md-6">
 
           <div class="col-md-6">
 
             <ul class="breadcrumbs">
 
             <ul class="breadcrumbs">
 
               <li><a href="https://2015.igem.org/Team:Peking">Home</a></li>
 
               <li><a href="https://2015.igem.org/Team:Peking">Home</a></li>
−
               <li>Achievements</li>
+
               <li>Parts</li>
 
             </ul>
 
             </ul>
 
           </div>
 
           </div>
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     <!-- End Page Banner -->
 
     <!-- End Page Banner -->
  
 +
 
     <!-- Start Content -->
 
     <!-- Start Content -->
 
     <div id="content">
 
     <div id="content">
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                 </li>
 
                 </li>
 
                 <li>
 
                 <li>
−
                   <a href="https://2015.igem.org/Team:Peking/Description">Parts Contribution</a>
+
                   <a href="https://2015.igem.org/Team:Peking/Part_Contribution">Parts Contribution</a>
 
                 </li>
 
                 </li>
 +
             
 
               </ul>
 
               </ul>
 
             </div>
 
             </div>
−
 
+
  <div id="sidebar2"class="widget widget-categories">
−
            <div id="sidebar2"class="widget widget-categories">
+
               <h4 style="font-size:18px">Parts <span class="head-line"></span></h4>
−
               <h4>Parts <span class="head-line"></span></h4>
+
 
                 <ul>
 
                 <ul>
 
                 <li>
 
                 <li>
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                 </li>
 
                 </li>
 
                 <li>
 
                 <li>
−
                   <a href="https://2015.igem.org/Team:Peking/Description">Parts Contribution</a>
+
                   <a href="https://2015.igem.org/Team:Peking/Part_Contribution">Parts Contribution</a>
 
                 </li>
 
                 </li>
 +
             
 
               </ul>
 
               </ul>
 
             </div>
 
             </div>
 +
         
 
           </div>
 
           </div>
 
           <!--End sidebar-->
 
           <!--End sidebar-->
  
 
           <!-- Page Content -->
 
           <!-- Page Content -->
−
           <div class="col-md-9 page-content">
+
           <div class="col-md-9 page-content" style="text-align:justify;">
−
             <div id="Part-Favorite part">
+
             <div id="Part-Favorite part" id="Document">
 
             <!-- Classic Heading -->
 
             <!-- Classic Heading -->
−
               <h3 class="classic-title"><span>Favorite Parts</span></h3>
+
               <h3 class="classic-title" style="margin-top:50px"><span>Favorite Parts</span></h3>
−
               <img src="https://static.igem.org/mediawiki/2015/0/08/Peking-team-about-01.jpg" style="width:100%">
+
<div>
−
              <p>Nluc-dCas9 fusion protein</p>
+
              <p><b>Nluc-dCas9 fusion protein</b><br/>
−
              <p>(BBa_K1689010)</p>
+
              <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a></p>
−
               <p>Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Cluc-dCas9 fusion protein ( BBa_K1689009) and sgRNAs, our paired dCas9 reporter system were constructed.</p>
+
              <div class="row">
−
               <p>Cluc-dCas9 fusion protein</p>
+
               
−
               <p>( BBa_K1689009)</p>
+
                  <div class="col-md-8">
−
               <p>Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Nluc-dCas9 fusion protein ( BBa_K1689010) and sgRNAs, our paired dCas9 reporter system were constructed.</p>
+
               <img src="https://static.igem.org/mediawiki/2015/a/a2/Peking-Parts-ND-long.png" style="max-height:200px">
−
               <p>sgRNA generator</p>
+
            </div>
−
               <p>(BBa_K1689000)</p>
+
            <div class="col-md-2"></div>
 +
          </div>
 +
               <p>Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Cluc-dCas9 fusion protein <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a> and sgRNAs, our paired dCas9 reporter system were constructed.</p>
 +
             
 +
               <p><b>Cluc-dCas9 fusion protein</b><br/>
 +
               <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a></p>
 +
              <div class="row">
 +
               
 +
                  <div class="col-md-8">
 +
              <img src="https://static.igem.org/mediawiki/2015/3/32/Peking-Parts-CD-long.png" style="max-height:200px">
 +
            </div>
 +
            <div class="col-md-4"></div>
 +
          </div>
 +
               <p>Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Nluc-dCas9 fusion protein <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a> and sgRNAs, our paired dCas9 reporter system were constructed.</p>
 +
             
 +
               <p><b>sgRNA generator</b><br/>
 +
               <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689000" style="color:#00afd1">(BBa_K1689000)</a></p>
 +
              <div class="row">
 +
               
 +
                  <div class="col-md-8">
 +
              <img src="https://static.igem.org/mediawiki/2015/f/f2/Peking-Parts-gRNA-long.png" style="max-height:200px">
 +
            </div>
 +
            <div class="col-md-4"></div>
 +
          </div>
 
               <p> sgRNA generator is used for generating sgRNA which recognizes targeted DNA, then guiding dCas9 to it.  
 
               <p> sgRNA generator is used for generating sgRNA which recognizes targeted DNA, then guiding dCas9 to it.  
 
</p>
 
</p>
Line 257: Line 256:
 
               </div>
 
               </div>
  
−
                 <h3 class="classic-title"><span>Part List</span></h3>
+
                 <h3 class="classic-title" style="margin-top:50px"><span>Part List</span></h3>
−
                <h4>Parts for paired dCas9 reporter system</h4>
+
<div><!--
 +
<div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>-->
 +
<div class="col-md-12">
 +
  <table class="table table-hover table-striped" style="color:#666;font-size:14px">
 +
<thead>
 +
<tr>
 +
<th>Favorite</th>
 +
<th>Name</th>
 +
<th>Type</th>
 +
<th>Description</th>
 +
<th>Design</th>
 +
</tr>
 +
</thead>  
  
−
                 <p>Nluc416-FRB(BBa_K1689003), FKBP-Cluc398(BBa_K1689005), Nluc398-FRB(BBa_K1689004) and FKBP-Cluc394(BBa_K1689006) are used to validate the reconsitituation activity of split luciferase.</p>
+
<tbody>
−
                 <p>Nluc-dCas9 (BBa_K1689010), Cluc-dCas9 ( BBa_K1689009), dCas9-Nluc(BBa_K1689008) and dCas9-Cluc (BBa_K1689007) fusion proteins are used for constructing paired dCas9 reporter system.</p>
+
<tr>
−
                 <p>Δα-dCas9(BBa_K1689011), dCas9-Nlact(BBa_K1689012), Nlact-dCas9(BBa_K1689013), dCas9-Clact(BBa_K1689014), F[1,2]-dCas9(BBa_K1689015), dCas9- F[1,2](BBa_K1689016), F3-dCas9(BBa_K1689017),  dCas9- F3(BBa_K1689018), dCas9-Δα(BBa_K1689019), delta omega-dCas9(BBa_K1689020) are used for further development of dectection.</p>
+
  <td>&nbsp;&radic;</td>
−
                 <h4>Parts for Molecular Beacon</h4>
+
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689000" style="color:#00afd1">BBa_K1689000</a></td>
−
                 <p>STV-Nluc(BBa_K1689001) and Cluc-STV(BBa_K1689002) are used for binding to molecular beocon.</p>
+
<td>Composite</td>
−
                 <h4>Parts for sgRNA generation</h4>
+
<td>sgRNA generator</td>
−
                 <p>sgRNA generator(BBa_K1689000) is used for generating sgRNA</p>
+
<td>LIU Wenchao, XU Luze</td>
−
                </div>
+
</tr>
 +
 
 +
<tr>
 +
  <td>&nbsp;&radic;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">BBa_K1689009</a></td>
 +
<td>Composite</td>
 +
<td>C-luc-dCas9</td>
 +
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&nbsp;&radic;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">BBa_K1689010</a></td>
 +
<td>Compostie</td>
 +
<td>N-luc-dCas9</td>
 +
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 +
</tr>
 +
 
 +
<tr>
 +
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">BBa_K1689001</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of STV-N-luc</td>
 +
<td>LI Xiang, LI Jiaofeng</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">BBa_K1689002</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of Cluc-STV</td>
 +
<td>LI Xiang, LI Jiaofeng</td>
 +
</tr>
 +
 
 +
<tr>
 +
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003" style="color:#00afd1">BBa_K1689003</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of Nluc416-FRB</td>
 +
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> 
 +
</tr>
 +
 
 +
<tr>
 +
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004" style="color:#00afd1">BBa_K1689004</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of Nluc398-FRB</td>
 +
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> 
 +
</tr>
 +
 
 +
<tr>
 +
<td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005" style="color:#00afd1">BBa_K1689005</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of FKBP-Cluc398</td>
 +
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> 
 +
</tr>
 +
 
 +
<tr>
 +
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006" style="color:#00afd1">BBa_K1689006</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of FKBP-Cluc394</td>
 +
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> 
 +
</tr>
 +
 
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">BBa_K1689007</a></td>
 +
<td>Composite</td>
 +
<td>dCas9-C-luc</td>
 +
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 +
</tr>
 +
 
 +
<tr>
 +
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">BBa_K1689008</a></td>
 +
<td>Composite</td>
 +
<td>dCas9-N-luc</td>
 +
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">BBa_K1689011</a></td>
 +
<td>Composite</td>
 +
<td>dCas9-delta alpha</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">BBa_K1689012</a></td>
 +
<td>Composite</td>
 +
<td>dCas9-Nlact</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013" style="color:#00afd1">BBa_K1689013</a></td>
 +
<td>Compostie</td>
 +
<td>Nlact-dCas9</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">BBa_K1689014</a></td>
 +
<td>Coding</td>
 +
<td>dCas9-Clact</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">BBa_K1689015</a></td>
 +
<td>Coding</td>
 +
<td>F[1,2]-dCas9</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">BBa_K1689016</a></td>
 +
<td>Coding</td>
 +
<td>dCas9-F[1,2]</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">BBa_K1689017</a></td>
 +
<td>Coding</td>
 +
<td>F3-dCas9</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">BBa_K1689018</a></td>
 +
<td>Coding</td>
 +
<td>dCas9-F3</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">BBa_K1689019</a></td>
 +
<td>Coding</td>
 +
<td>delta alpha-dCas9</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">BBa_K1689020</a></td>
 +
<td>Coding</td>
 +
<td>dCas9-delta omega</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
</table>
 +
</div>
 +
 
 +
                <p><b>Parts for paired dCas9 reporter system</b></p>
 +
 
 +
                 <p>Nluc416-FRB <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003" style="color:#00afd1">(BBa_K1689003)</a>,<br/> FKBP-Cluc398 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005" style="color:#00afd1">(BBa_K1689005)</a>,<br/> Nluc398-FRB<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004" style="color:#00afd1"> (BBa_K1689004)</a>, <br/>FKBP-Cluc394<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006" style="color:#00afd1"> (BBa_K1689006)</a><br/> are used to validate the reconsitituation activity of split luciferase.</p>
 +
                 <p>Nluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a>,<br/> Cluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a>,<br/> dCas9-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">(BBa_K1689008)</a>,<br/> dCas9-Cluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689007)</a><br/> fusion proteins are used for constructing paired dCas9 reporter system.</p>
 +
                 <p>&Delta;α-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689011)</a>,<br/> dCas9-Nlact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">(BBa_K1689012)</a>,<br/> Nlact-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689013)</a>,<br/> dCas9-Clact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689014)</a>,<br/> F[1,2]-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">(BBa_K1689015)</a>,<br/> dCas9-F[1,2] <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">(BBa_K1689016)</a>, <br/>F3-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">(BBa_K1689017)</a>,<br/> dCas9-F3 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">(BBa_K1689018)</a>,<br/> dCas9-&Delta;&alpha;<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1"> (BBa_K1689019)</a>,<br/> &Delta;&omega;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a><br/> are used for further development of dectection.</p>
 +
                 <p><b>Parts for Molecular Beacon</b></p>
 +
                 <p>STV-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">(BBa_K1689001)</a>,<br/> Cluc-STV <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">(BBa_K1689002)</a><br/> are used for binding to molecular beacon.</p>
 +
</div>
 +
                 <p id="Collection"><b>Parts for sgRNA generation</b></p>
 +
<div>
 +
                 <p>sgRNA generator <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689000" style="color:#00afd1">(BBa_K1689000)</a> is used for generating sgRNA</p>
 +
             
 +
</div>
 +
                <h3 class="classic-title" style="margin-top:50px"><span>Part Collection</span></h3>
 
<div>
 
<div>
−
                <h3 class="classic-title"><span>Part collection</span></h3>
+
                 <p>2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explored a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the  form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.</p>
−
                 <p>2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explore a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the  form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.</p>
+
 
                 <p>We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".</p>
 
                 <p>We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".</p>
 
                 <p>The Collection includes:</p>
 
                 <p>The Collection includes:</p>
−
                 <p>dCas9-split luciferase Parts</p>
+
 
−
                 <p> Nluc-dCas9 (BBa_K1689010)</p>
+
                 <p><b>dCas9-split luciferase Parts</b></p>
−
                <p>Cluc-dCas9 ( BBa_K1689009)</p>
+
                 <p>Nluc-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a><br/>
−
                <p> dCas9-Nluc(BBa_K1689008)</p>
+
                Cluc-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a><br/>
−
                <p>dCas9-Cluc (BBa_K1689007)</p>
+
                dCas9-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">(BBa_K1689008)</a><br/>
−
                 <p>dCas9-split Dihydrofolate Reductase Parts</p>
+
                dCas9-Cluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689007)</a></p>
−
                 <p> F[1,2]-dCas9(BBa_K1689015)</p>
+
 
−
                <p>F3-dCas9(BBa_K1689017)</p>
+
                 <p><b>dCas9-split Dihydrofolate Reductase Parts</b></p>
−
                <p>dCas9-F[1,2](BBa_K1689016)</p>
+
                 <p>F[1,2]-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">(BBa_K1689015)</a><br/>
−
                <p>dCas9-F3(BBa_K1689018)</p>
+
                F3-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689017)</a><br/>
−
                 <p>dCas9-split β–Lactamase Parts</p>
+
                dCas9-F[1,2] <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">(BBa_K1689016)</a><br/>
−
                 <p> dCas9-Nlact(BBa_K1689012)</p>
+
                dCas9-F3 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">(BBa_K1689018)</a></p>
−
                <p>Nlact-dCas9(BBa_K1689013)</p>
+
 
−
                <p> dCas9-Clact(BBa_K1689014)</p>
+
                 <p><b>dCas9-split β–Lactamase Parts</b></p>
−
                 <p>dCas9-splitβ–Galactosidase Parts</p>
+
                 <p>dCas9-Nlact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">(BBa_K1689012)</a><br/>
−
                 <p> delta alpha-dCas9(BBa_K1689011)</p>
+
                Nlact-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013" style="color:#00afd1">(BBa_K1689013)</a><br/>
−
                <p>dCas9-delta alpha(BBa_K1689019)</p>
+
                dCas9-Clact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689014)</a></p>
−
                <p>delta omega-dCas9(BBa_K1689020)</p>
+
               
 +
                 <p><b>dCas9-splitβ–Galactosidase Parts</b></p>
 +
                 <p>&Delta;&alpha;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">(BBa_K1689011)</a><br/>
 +
                dCas9-&Delta;&alpha; <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">(BBa_K1689019)</a><br/>
 +
                &Delta;&omega;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a></p>
 
                 <p>Among them, the PC reporters with split luciferase oxidizes luciferin and gives out bioluminescence signal, while the other three reporters take electric signal as their output, which is also able to be detected and qualified.</p>
 
                 <p>Among them, the PC reporters with split luciferase oxidizes luciferin and gives out bioluminescence signal, while the other three reporters take electric signal as their output, which is also able to be detected and qualified.</p>
 
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−
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+
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                                 <li><span>Address:</span> Peking University, <br>No.5 Yiheyuan Road Haidian District, <br>Beijing, P.R.China 100871</li>
 
                                 <li><span>Address:</span> Peking University, <br>No.5 Yiheyuan Road Haidian District, <br>Beijing, P.R.China 100871</li>
 
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Latest revision as of 00:40, 20 November 2015

Peking iGEM 2015

Parts

Each part gives us a new experience.

Favorite Parts

Nluc-dCas9 fusion protein
(BBa_K1689010)

Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Cluc-dCas9 fusion protein (BBa_K1689009) and sgRNAs, our paired dCas9 reporter system were constructed.

Cluc-dCas9 fusion protein
(BBa_K1689009)

Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Nluc-dCas9 fusion protein (BBa_K1689010) and sgRNAs, our paired dCas9 reporter system were constructed.

sgRNA generator
(BBa_K1689000)

sgRNA generator is used for generating sgRNA which recognizes targeted DNA, then guiding dCas9 to it.

Part List

Favorite Name Type Description Design
 √ BBa_K1689000 Composite sgRNA generator LIU Wenchao, XU Luze
 √ BBa_K1689009 Composite C-luc-dCas9 LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
 √ BBa_K1689010 Compostie N-luc-dCas9 LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
  BBa_K1689001 Coding Coding sequence of STV-N-luc LI Xiang, LI Jiaofeng
  BBa_K1689002 Coding Coding sequence of Cluc-STV LI Xiang, LI Jiaofeng
  BBa_K1689003 Coding Coding sequence of Nluc416-FRB WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689004 Coding Coding sequence of Nluc398-FRB WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689005 Coding Coding sequence of FKBP-Cluc398 WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689006 Coding Coding sequence of FKBP-Cluc394 WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689007 Composite dCas9-C-luc LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
  BBa_K1689008 Composite dCas9-N-luc LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
  BBa_K1689011 Composite dCas9-delta alpha ZHANG Yihao, WEI Weijia
  BBa_K1689012 Composite dCas9-Nlact ZHANG Yihao, WEI Weijia
  BBa_K1689013 Compostie Nlact-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689014 Coding dCas9-Clact ZHANG Yihao, WEI Weijia
  BBa_K1689015 Coding F[1,2]-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689016 Coding dCas9-F[1,2] ZHANG Yihao, WEI Weijia
  BBa_K1689017 Coding F3-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689018 Coding dCas9-F3 ZHANG Yihao, WEI Weijia
  BBa_K1689019 Coding delta alpha-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689020 Coding dCas9-delta omega ZHANG Yihao, WEI Weijia

Parts for paired dCas9 reporter system

Nluc416-FRB (BBa_K1689003),
FKBP-Cluc398 (BBa_K1689005),
Nluc398-FRB (BBa_K1689004),
FKBP-Cluc394 (BBa_K1689006)
are used to validate the reconsitituation activity of split luciferase.

Nluc-dCas9(BBa_K1689010),
Cluc-dCas9(BBa_K1689009),
dCas9-Nluc (BBa_K1689008),
dCas9-Cluc (BBa_K1689007)
fusion proteins are used for constructing paired dCas9 reporter system.

Δα-dCas9 (BBa_K1689011),
dCas9-Nlact (BBa_K1689012),
Nlact-dCas9 (BBa_K1689013),
dCas9-Clact (BBa_K1689014),
F[1,2]-dCas9 (BBa_K1689015),
dCas9-F[1,2] (BBa_K1689016),
F3-dCas9 (BBa_K1689017),
dCas9-F3 (BBa_K1689018),
dCas9-Δα (BBa_K1689019),
Δω-dCas9 (BBa_K1689020)
are used for further development of dectection.

Parts for Molecular Beacon

STV-Nluc (BBa_K1689001),
Cluc-STV (BBa_K1689002)
are used for binding to molecular beacon.

Parts for sgRNA generation

sgRNA generator (BBa_K1689000) is used for generating sgRNA

Part Collection

2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explored a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.

We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".

The Collection includes:

dCas9-split luciferase Parts

Nluc-dCas9 (BBa_K1689010)
Cluc-dCas9 (BBa_K1689009)
dCas9-Nluc (BBa_K1689008)
dCas9-Cluc (BBa_K1689007)

dCas9-split Dihydrofolate Reductase Parts

F[1,2]-dCas9 (BBa_K1689015)
F3-dCas9 (BBa_K1689017)
dCas9-F[1,2] (BBa_K1689016)
dCas9-F3 (BBa_K1689018)

dCas9-split β–Lactamase Parts

dCas9-Nlact (BBa_K1689012)
Nlact-dCas9 (BBa_K1689013)
dCas9-Clact (BBa_K1689014)

dCas9-splitβ–Galactosidase Parts

Δα-dCas9 (BBa_K1689011)
dCas9-Δα (BBa_K1689019)
Δω-dCas9 (BBa_K1689020)

Among them, the PC reporters with split luciferase oxidizes luciferin and gives out bioluminescence signal, while the other three reporters take electric signal as their output, which is also able to be detected and qualified.