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| <div class="navbar-collapse collapse" style="padding-top:5px;padding-bottom:0"> | | <div class="navbar-collapse collapse" style="padding-top:5px;padding-bottom:0"> |
| <!-- Start Navigation List --> | | <!-- Start Navigation List --> |
− | <ul class="nav navbar-nav navbar-right " style="padding-bottom:15px;height:88px;padding-top:10px"> | + | <ul class="nav navbar-nav navbar-right " style="padding-bottom:15px;padding-top:10px"> |
| <li> | | <li> |
| <a href="https://2015.igem.org/Team:Peking/JudgingCriteria" class="active">Achievements</a> | | <a href="https://2015.igem.org/Team:Peking/JudgingCriteria" class="active">Achievements</a> |
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| <div class="col-md-6"> | | <div class="col-md-6"> |
| <h2 style="font-size:20px; margin-bottom:5px; padding-bottom:0" >P<span style="text-transform:lowercase">arts</span></h2> | | <h2 style="font-size:20px; margin-bottom:5px; padding-bottom:0" >P<span style="text-transform:lowercase">arts</span></h2> |
− | <p style="margin-top:0px;font-size:14px" id="Overview">Each part give us a new experience.</p> | + | <p style="margin-top:0px;font-size:14px" id="Overview">Each part gives us a new experience.</p> |
| </div> | | </div> |
| <div class="col-md-6"> | | <div class="col-md-6"> |
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| <a href="https://2015.igem.org/Team:Peking/Parts" style="color:#00afd1">Parts Document</a> | | <a href="https://2015.igem.org/Team:Peking/Parts" style="color:#00afd1">Parts Document</a> |
| </li> | | </li> |
− | | + | <li> |
| + | <a href="https://2015.igem.org/Team:Peking/Basic_Part">Basic Parts</a> |
| + | </li> |
| + | <li> |
| + | <a href="https://2015.igem.org/Team:Peking/Composite_Part">Composite Parts</a> |
| + | </li> |
| <li> | | <li> |
| <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a> | | <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a> |
| + | </li> |
| + | <li> |
| + | <a href="https://2015.igem.org/Team:Peking/Part_Contribution">Parts Contribution</a> |
| </li> | | </li> |
| | | |
| </ul> | | </ul> |
| </div> | | </div> |
− | | + | <div id="sidebar2"class="widget widget-categories"> |
− | <div id="sidebar2"class="widget widget-categories">
| + | <h4 style="font-size:18px">Parts <span class="head-line"></span></h4> |
− | <h4>Parts <span class="head-line"></span></h4> | + | |
| <ul> | | <ul> |
| <li> | | <li> |
| <a href="https://2015.igem.org/Team:Peking/Parts" style="color:#00afd1">Parts Document</a> | | <a href="https://2015.igem.org/Team:Peking/Parts" style="color:#00afd1">Parts Document</a> |
| </li> | | </li> |
− | | + | <li> |
| + | <a href="https://2015.igem.org/Team:Peking/Basic_Part">Basic Parts</a> |
| + | </li> |
| + | <li> |
| + | <a href="https://2015.igem.org/Team:Peking/Composite_Part">Composite Parts</a> |
| + | </li> |
| <li> | | <li> |
| <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a> | | <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a> |
| + | </li> |
| + | <li> |
| + | <a href="https://2015.igem.org/Team:Peking/Part_Contribution">Parts Contribution</a> |
| </li> | | </li> |
| | | |
| </ul> | | </ul> |
| </div> | | </div> |
| + | |
| </div> | | </div> |
| <!--End sidebar--> | | <!--End sidebar--> |
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| | | |
| <h3 class="classic-title" style="margin-top:50px"><span>Part List</span></h3> | | <h3 class="classic-title" style="margin-top:50px"><span>Part List</span></h3> |
− | <!--<div> | + | <div><!-- |
| <div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>--> | | <div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>--> |
| <div class="col-md-12"> | | <div class="col-md-12"> |
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| <td>Composite</td> | | <td>Composite</td> |
| <td>sgRNA generator</td> | | <td>sgRNA generator</td> |
− | <td>ZHAO Xuejin</td> | + | <td>LIU Wenchao, XU Luze</td> |
− | </tr>
| + | |
− | | + | |
− | <tr>
| + | |
− | <td> √</td>
| + | |
− | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">BBa_K1689008</a></td>
| + | |
− | <td>Composite</td>
| + | |
− | <td>Coding sequence of dCas9-Nluc</td>
| + | |
− | <td>ZHANG Yihao</td>
| + | |
| </tr> | | </tr> |
| | | |
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| <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">BBa_K1689009</a></td> | | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">BBa_K1689009</a></td> |
| <td>Composite</td> | | <td>Composite</td> |
− | <td>Coding sequence Cluc-dCas9</td> | + | <td>C-luc-dCas9</td> |
− | <td>ZHANG Yihao</td> | + | <td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td> |
| </tr> | | </tr> |
| | | |
| <tr> | | <tr> |
− | <td> </td> | + | <td> √</td> |
| <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">BBa_K1689010</a></td> | | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">BBa_K1689010</a></td> |
| <td>Compostie</td> | | <td>Compostie</td> |
− | <td>Coding sequence of N-luc-dCas9</td> | + | <td>N-luc-dCas9</td> |
− | <td>ZHANG Yihao</td> | + | <td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td> |
| </tr> | | </tr> |
| | | |
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| <td>Coding</td> | | <td>Coding</td> |
| <td>Coding sequence of STV-N-luc</td> | | <td>Coding sequence of STV-N-luc</td> |
− | <td>ZHANG Yihao</td> | + | <td>LI Xiang, LI Jiaofeng</td> |
| </tr> | | </tr> |
| | | |
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| <td>Coding</td> | | <td>Coding</td> |
| <td>Coding sequence of Cluc-STV</td> | | <td>Coding sequence of Cluc-STV</td> |
− | <td>ZHANG Yihao</td> | + | <td>LI Xiang, LI Jiaofeng</td> |
| </tr> | | </tr> |
| | | |
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| <td>Coding</td> | | <td>Coding</td> |
| <td>Coding sequence of Nluc416-FRB</td> | | <td>Coding sequence of Nluc416-FRB</td> |
− | <td>Xiang Li</td> | + | <td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> |
| </tr> | | </tr> |
| | | |
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| <td>Coding</td> | | <td>Coding</td> |
| <td>Coding sequence of Nluc398-FRB</td> | | <td>Coding sequence of Nluc398-FRB</td> |
− | <td>Xiang Li</td> | + | <td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> |
| </tr> | | </tr> |
| | | |
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| <td>Coding</td> | | <td>Coding</td> |
| <td>Coding sequence of FKBP-Cluc398</td> | | <td>Coding sequence of FKBP-Cluc398</td> |
− | <td>Xiang Li</td> | + | <td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> |
| </tr> | | </tr> |
| | | |
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| <td>Coding</td> | | <td>Coding</td> |
| <td>Coding sequence of FKBP-Cluc394</td> | | <td>Coding sequence of FKBP-Cluc394</td> |
− | <td>Xiang Li</td> | + | <td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> |
| </tr> | | </tr> |
| | | |
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| <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">BBa_K1689007</a></td> | | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">BBa_K1689007</a></td> |
| <td>Composite</td> | | <td>Composite</td> |
− | <td>Coding sequence of dCas9-C-luc</td> | + | <td>dCas9-C-luc</td> |
− | <td>ZHANG Yihao</td> | + | <td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td> </td> |
| + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">BBa_K1689008</a></td> |
| + | <td>Composite</td> |
| + | <td>dCas9-N-luc</td> |
| + | <td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td> |
| </tr> | | </tr> |
| | | |
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| <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">BBa_K1689011</a></td> | | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">BBa_K1689011</a></td> |
| <td>Composite</td> | | <td>Composite</td> |
− | <td>delta alpha segment of lacz fused with dCas9, His tag allows for protein purification</td> | + | <td>dCas9-delta alpha</td> |
− | <td>Jiaofeng Li</td> | + | <td>ZHANG Yihao, WEI Weijia</td> |
| </tr> | | </tr> |
| | | |
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| <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">BBa_K1689012</a></td> | | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">BBa_K1689012</a></td> |
| <td>Composite</td> | | <td>Composite</td> |
− | <td>N terminal of β-lactamase fused with dCas9</td> | + | <td>dCas9-Nlact</td> |
− | <td>Jiaofeng Li</th> | + | <td>ZHANG Yihao, WEI Weijia</td> |
| </tr> | | </tr> |
| | | |
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| <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013" style="color:#00afd1">BBa_K1689013</a></td> | | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013" style="color:#00afd1">BBa_K1689013</a></td> |
| <td>Compostie</td> | | <td>Compostie</td> |
− | <td>NFluc of lactamase fused with dCas9, His tag allows for protein purification</td> | + | <td>Nlact-dCas9</td> |
− | <td>Jiaofeng Li</td> | + | <td>ZHANG Yihao, WEI Weijia</td> |
| </tr> | | </tr> |
| | | |
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| <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">BBa_K1689014</a></td> | | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">BBa_K1689014</a></td> |
| <td>Coding</td> | | <td>Coding</td> |
− | <td>dCas9 fused with C terminal of beta-lactamase</td> | + | <td>dCas9-Clact</td> |
− | <td>Wei Weijia</td> | + | <td>ZHANG Yihao, WEI Weijia</td> |
| </tr> | | </tr> |
| | | |
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| <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">BBa_K1689015</a></td> | | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">BBa_K1689015</a></td> |
| <td>Coding</td> | | <td>Coding</td> |
− | <td>segment F[1,2] of DHFR-dCas9 fusion protein</td> | + | <td>F[1,2]-dCas9</td> |
− | <td>Wei Weijia</td> | + | <td>ZHANG Yihao, WEI Weijia</td> |
| </tr> | | </tr> |
| | | |
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| <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">BBa_K1689016</a></td> | | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">BBa_K1689016</a></td> |
| <td>Coding</td> | | <td>Coding</td> |
− | <td>fusion protein of dCas9-F[1,2]</td> | + | <td>dCas9-F[1,2]</td> |
− | <td>Wei Weijia</td> | + | <td>ZHANG Yihao, WEI Weijia</td> |
| </tr> | | </tr> |
| | | |
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| <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">BBa_K1689017</a></td> | | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">BBa_K1689017</a></td> |
| <td>Coding</td> | | <td>Coding</td> |
− | <td>DHFR F3 fragment-dCas9 fusion protein</td> | + | <td>F3-dCas9</td> |
− | <td>Wei Weijia</td> | + | <td>ZHANG Yihao, WEI Weijia</td> |
| </tr> | | </tr> |
| | | |
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| <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">BBa_K1689018</a></td> | | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">BBa_K1689018</a></td> |
| <td>Coding</td> | | <td>Coding</td> |
− | <td>dCas9 fused with F3</td> | + | <td>dCas9-F3</td> |
− | <td>Wei Weijia</td> | + | <td>ZHANG Yihao, WEI Weijia</td> |
| </tr> | | </tr> |
| | | |
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| <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">BBa_K1689019</a></td> | | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">BBa_K1689019</a></td> |
| <td>Coding</td> | | <td>Coding</td> |
− | <td>Δα segment of β-galactosidase fused with dCas9</td> | + | <td>delta alpha-dCas9</td> |
− | <td>Wei Weijia</td> | + | <td>ZHANG Yihao, WEI Weijia</td> |
| </tr> | | </tr> |
| | | |
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| <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">BBa_K1689020</a></td> | | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">BBa_K1689020</a></td> |
| <td>Coding</td> | | <td>Coding</td> |
− | <td>dCas9 fused with delta omega segment of β-galactosidase</td> | + | <td>dCas9-delta omega</td> |
− | <td>Wei Weijia</td> | + | <td>ZHANG Yihao, WEI Weijia</td> |
| </tr> | | </tr> |
− | </table> | + | </table> |
| </div> | | </div> |
| | | |
| <p><b>Parts for paired dCas9 reporter system</b></p> | | <p><b>Parts for paired dCas9 reporter system</b></p> |
| | | |
− | <p>Nluc416-FRB <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003" style="color:#00afd1">(BBa_K1689003)</a>, FKBP-Cluc398 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005" style="color:#00afd1">(BBa_K1689005)</a>, Nluc398-FRB<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004" style="color:#00afd1"> (BBa_K1689004)</a> and FKBP-Cluc394<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006" style="color:#00afd1"> (BBa_K1689006)</a> are used to validate the reconsitituation activity of split luciferase.</p> | + | <p>Nluc416-FRB <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003" style="color:#00afd1">(BBa_K1689003)</a>,<br/> FKBP-Cluc398 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005" style="color:#00afd1">(BBa_K1689005)</a>,<br/> Nluc398-FRB<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004" style="color:#00afd1"> (BBa_K1689004)</a>, <br/>FKBP-Cluc394<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006" style="color:#00afd1"> (BBa_K1689006)</a><br/> are used to validate the reconsitituation activity of split luciferase.</p> |
− | <p>Nluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a>, Cluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a>, dCas9-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">(BBa_K1689008)</a> and dCas9-Cluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689007)</a> fusion proteins are used for constructing paired dCas9 reporter system.</p> | + | <p>Nluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a>,<br/> Cluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a>,<br/> dCas9-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">(BBa_K1689008)</a>,<br/> dCas9-Cluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689007)</a><br/> fusion proteins are used for constructing paired dCas9 reporter system.</p> |
− | <p>Δα-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689011)</a>, dCas9-Nlact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">(BBa_K1689012)</a>, Nlact-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689013)</a>, dCas9-Clact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689014)</a>, F[1,2]-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">(BBa_K1689015)</a>, dCas9-F[1,2] <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">(BBa_K1689016)</a>, F3-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">(BBa_K1689017)</a>, dCas9-F3 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">(BBa_K1689018)</a>, dCas9-Δα<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1"> (BBa_K1689019)</a>, Δω-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a> are used for further development of dectection.</p> | + | <p>Δα-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689011)</a>,<br/> dCas9-Nlact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">(BBa_K1689012)</a>,<br/> Nlact-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689013)</a>,<br/> dCas9-Clact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689014)</a>,<br/> F[1,2]-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">(BBa_K1689015)</a>,<br/> dCas9-F[1,2] <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">(BBa_K1689016)</a>, <br/>F3-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">(BBa_K1689017)</a>,<br/> dCas9-F3 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">(BBa_K1689018)</a>,<br/> dCas9-Δα<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1"> (BBa_K1689019)</a>,<br/> Δω-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a><br/> are used for further development of dectection.</p> |
| <p><b>Parts for Molecular Beacon</b></p> | | <p><b>Parts for Molecular Beacon</b></p> |
− | <p>STV-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">(BBa_K1689001)</a> and Cluc-STV <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">(BBa_K1689002)</a> are used for binding to molecular beacon.</p> | + | <p>STV-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">(BBa_K1689001)</a>,<br/> Cluc-STV <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">(BBa_K1689002)</a><br/> are used for binding to molecular beacon.</p> |
| </div> | | </div> |
| <p id="Collection"><b>Parts for sgRNA generation</b></p> | | <p id="Collection"><b>Parts for sgRNA generation</b></p> |
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| <h3 class="classic-title" style="margin-top:50px"><span>Part Collection</span></h3> | | <h3 class="classic-title" style="margin-top:50px"><span>Part Collection</span></h3> |
| <div> | | <div> |
− | <p>2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explore a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.</p> | + | <p>2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explored a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.</p> |
| <p>We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".</p> | | <p>We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".</p> |
| <p>The Collection includes:</p> | | <p>The Collection includes:</p> |