Difference between revisions of "Team:Peking/Parts"

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{{Peking}}
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  <meta charset="utf-8">
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  <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1">
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  <meta name="Peking-iGEM-2015" content="iGEM - Peking iGEM 2015">
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    /* to style the sidebar*/
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<h2> Part Documentation</h2>
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    </style>
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</head>
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
<body>
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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  <!-- Container -->
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  <div id="container">
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    <!-- Start Header -->
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    <header class="clearfix">
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        <!-- Start  Logo & Naviagtion  -->
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            <div class="navbar navbar-default navbar-top">
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                <div class="container">
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                    <div class="navbar-header" >
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                        <!-- Stat Toggle Nav Link For Mobiles -->
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                        <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-collapse" style="margin-top:40px">
 +
                            <i class="fa"><img  style="margin-left:0;height:30px;"src="https://static.igem.org/mediawiki/2015/4/45/Peking-Hambergur-1.png" ></i>
 +
                        </button>
 +
                        <!-- End Toggle Nav Link For Mobiles -->
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                        <a class="navbar-brand" href="https://2015.igem.org/Team:Peking">
 +
                            <img alt="Peking iGEM 2015" src="https://static.igem.org/mediawiki/2015/8/8b/Peking-header-logo-1.png" style="height:55px;margin-top:7px">
 +
                        </a>
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                    </div>
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                    <div class="navbar-collapse collapse"  style="padding-top:5px;padding-bottom:0">
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                        <!-- Start Navigation List -->
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                        <ul class="nav navbar-nav navbar-right " style="padding-bottom:15px;padding-top:10px">
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                            <li>
 +
                                <a href="https://2015.igem.org/Team:Peking/JudgingCriteria" class="active">Achievements</a>
 +
                                <ul class="dropdown">
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/JudgingCriteria">Judging Criteria</a>
 +
                                    </li>
 +
                                    <li><a class="active" href="https://2015.igem.org/Team:Peking/Parts">Parts</a>
 +
                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Collaborations">Collaborations</a>
 +
                                    </li>
 +
                                </ul>
 +
                            </li>
 +
                            <li>
 +
                                <a href="https://2015.igem.org/Team:Peking/Design">Project</a>
 +
                                <ul class="dropdown">
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Design">Overview</a>
 +
                                    </li>
 +
<li><a href="https://2015.igem.org/Team:Peking/Design/PC_Reporter">Paired<span style="text-transform:lowercase"> d</span>Cas9 Reporter</a>                                   
 +
</li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Design/Isothermal">Iso-<span style="text-transform:lowercase">t</span>hermal Amplification</a></li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Device">Hardware</a>
 +
                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Design/Speculation">Speculation</a>
 +
                                    </li>
 +
                                </ul>
 +
                            </li>
 +
                            <li>
 +
                                <a href="https://2015.igem.org/Team:Peking/Modeling">Modeling</a>
 +
                                <ul class="dropdown">
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Modeling">Array Design</a>
 +
                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Modeling/Analysis">Analysis algorithm</a>
 +
                                    </li>
 +
                                </ul>
 +
                            </li>
 +
                            <li>
 +
                                <a href="https://2015.igem.org/Team:Peking/Practices">Practices</a>
 +
                                <ul class="dropdown">
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Practices">Overview</a>
 +
                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Practices/Background">TB Facts</a>
 +
                                    </li>
 +
                                    <li><a  href="https://2015.igem.org/Team:Peking/Practices/Consultation">Consultation and Interview</a>
 +
                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Practices/Engagement">Public Engagement</a>
 +
                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Practices/Outreach">Ethics and Economics Issues</a>
 +
                                    </li>
 +
                                </ul>
 +
                            </li>
 +
                            <li>
 +
                                <a href="https://2015.igem.org/Team:Peking/Team">Lab</a>
 +
                                <ul>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Team">Team</a>
 +
                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Notebook">Notebook</a>
 +
                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Attributions">Attributions</a>
 +
                                    </li>
 +
                                    <li><a href="https://2015.igem.org/Team:Peking/Safety">Safety</a>
 +
                                    </li>
 +
                                </ul>
 +
                            </li>
 +
                        </ul>
 +
                        <!-- End Navigation List -->
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                    </div>
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                </div>
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            </div>
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            <!-- End Header Logo & Naviagtion -->
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        </header>
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        <!-- End Header Section -->
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    <!-- Start Page Banner -->
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    <div class="page-banner">
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      <div class="container">
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        <div class="row">
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          <div class="col-md-6">
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            <h2 style="font-size:20px; margin-bottom:5px; padding-bottom:0" >P<span style="text-transform:lowercase">arts</span></h2>
 +
            <p style="margin-top:0px;font-size:14px" id="Overview">Each part gives us a new experience.</p>
 +
          </div>
 +
          <div class="col-md-6">
 +
            <ul class="breadcrumbs">
 +
              <li><a href="https://2015.igem.org/Team:Peking">Home</a></li>
 +
              <li>Parts</li>
 +
            </ul>
 +
          </div>
 +
        </div>
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      </div>
 +
    </div>
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    <!-- End Page Banner -->
  
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    <!-- Start Content -->
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    <div id="content">
 +
      <div class="container">
 +
        <div class="row blog-page">
 +
                 
 +
          <!--Sidebar-->
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          <div class="col-md-3 sidebar left-sidebar">
 +
            <!-- Categories Widget -->
 +
            <div id="sidebar1"class="widget widget-categories">
 +
              <h4 style="font-size:18px">Parts <span class="head-line"></span></h4>
 +
                <ul>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Parts" style="color:#00afd1">Parts Document</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Basic_Part">Basic Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Composite_Part">Composite Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Contribution">Parts Contribution</a>
 +
                </li>
 +
             
 +
              </ul>
 +
            </div>
 +
  <div id="sidebar2"class="widget widget-categories">
 +
              <h4 style="font-size:18px">Parts <span class="head-line"></span></h4>
 +
                <ul>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Parts" style="color:#00afd1">Parts Document</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Basic_Part">Basic Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Composite_Part">Composite Parts</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Collection">Parts Collection</a>
 +
                </li>
 +
                <li>
 +
                  <a href="https://2015.igem.org/Team:Peking/Part_Contribution">Parts Contribution</a>
 +
                </li>
 +
             
 +
              </ul>
 +
            </div>
 +
         
 +
          </div>
 +
          <!--End sidebar-->
  
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<div class="highlightBox">
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          <!-- Page Content -->
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<h4>Note</h4>
+
          <div class="col-md-9 page-content" style="text-align:justify;">
−
<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
+
            <div id="Part-Favorite part" id="Document">
−
</div>
+
            <!-- Classic Heading -->
 +
              <h3 class="classic-title" style="margin-top:50px"><span>Favorite Parts</span></h3>
 +
<div>
 +
              <p><b>Nluc-dCas9 fusion protein</b><br/>
 +
              <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a></p>
 +
              <div class="row">
 +
               
 +
                  <div class="col-md-8">
 +
              <img src="https://static.igem.org/mediawiki/2015/a/a2/Peking-Parts-ND-long.png" style="max-height:200px">
 +
            </div>
 +
            <div class="col-md-2"></div>
 +
          </div>
 +
              <p>Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Cluc-dCas9 fusion protein <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a> and sgRNAs, our paired dCas9 reporter system were constructed.</p>
 +
             
 +
              <p><b>Cluc-dCas9 fusion protein</b><br/>
 +
              <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a></p>
 +
              <div class="row">
 +
               
 +
                  <div class="col-md-8">
 +
              <img src="https://static.igem.org/mediawiki/2015/3/32/Peking-Parts-CD-long.png" style="max-height:200px">
 +
            </div>
 +
            <div class="col-md-4"></div>
 +
          </div>
 +
              <p>Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Nluc-dCas9 fusion protein <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a> and sgRNAs, our paired dCas9 reporter system were constructed.</p>
 +
             
 +
              <p><b>sgRNA generator</b><br/>
 +
              <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689000" style="color:#00afd1">(BBa_K1689000)</a></p>
 +
              <div class="row">
 +
               
 +
                  <div class="col-md-8">
 +
              <img src="https://static.igem.org/mediawiki/2015/f/f2/Peking-Parts-gRNA-long.png" style="max-height:200px">
 +
            </div>
 +
            <div class="col-md-4"></div>
 +
          </div>
 +
              <p> sgRNA generator is used for generating sgRNA which recognizes targeted DNA, then guiding dCas9 to it.
 +
</p>
 +
             
 +
              </div>
  
 +
                <h3 class="classic-title" style="margin-top:50px"><span>Part List</span></h3>
 +
<div><!--
 +
<div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading.....</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM015', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>-->
 +
<div class="col-md-12">
 +
  <table class="table table-hover table-striped" style="color:#666;font-size:14px">
 +
<thead>
 +
<tr>
 +
<th>Favorite</th>
 +
<th>Name</th>
 +
<th>Type</th>
 +
<th>Description</th>
 +
<th>Design</th>
 +
</tr>
 +
</thead>
  
 +
<tbody>
 +
<tr>
 +
  <td>&nbsp;&radic;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689000" style="color:#00afd1">BBa_K1689000</a></td>
 +
<td>Composite</td>
 +
<td>sgRNA generator</td>
 +
<td>LIU Wenchao, XU Luze</td>
 +
</tr>
  
−
<h4>Adding parts to the registry</h4>
+
<tr>  
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
  <td>&nbsp;&radic;</td>
−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
+
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">BBa_K1689009</a></td>  
 +
<td>Composite</td>  
 +
<td>C-luc-dCas9</td>  
 +
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 +
</tr>  
  
 +
<tr>
 +
<td>&nbsp;&radic;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">BBa_K1689010</a></td>
 +
<td>Compostie</td>
 +
<td>N-luc-dCas9</td>
 +
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 +
</tr>
  
−
<h4>What information do I need to start putting my parts on the Registry?</h4>
+
<tr>  
−
<p>The information needed to initially create a part on the Registry is:</p>
+
  <td>&nbsp;</td>  
−
<ul>
+
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">BBa_K1689001</a></td>  
−
<li>Part Name</li>
+
<td>Coding</td>  
−
<li>Part type</li>
+
<td>Coding sequence of STV-N-luc</td>  
−
<li>Creator</li>
+
<td>LI Xiang, LI Jiaofeng</td>  
−
<li>Sequence</li>
+
</tr>  
−
<li>Short Description (60 characters on what the DNA does)</li>
+
−
<li>Long Description (Longer description of what the DNA does)</li>
+
−
<li>Design considerations</li>
+
−
</ul>
+
  
−
<p>
+
<tr>
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
+
<td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">BBa_K1689002</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of Cluc-STV</td>
 +
<td>LI Xiang, LI Jiaofeng</td>
 +
</tr>  
  
 +
<tr>
 +
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003" style="color:#00afd1">BBa_K1689003</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of Nluc416-FRB</td>
 +
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> 
 +
</tr>
  
 +
<tr>
 +
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004" style="color:#00afd1">BBa_K1689004</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of Nluc398-FRB</td>
 +
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> 
 +
</tr>
  
 +
<tr>
 +
<td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005" style="color:#00afd1">BBa_K1689005</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of FKBP-Cluc398</td>
 +
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> 
 +
</tr>
  
 +
<tr>
 +
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006" style="color:#00afd1">BBa_K1689006</a></td>
 +
<td>Coding</td>
 +
<td>Coding sequence of FKBP-Cluc394</td>
 +
<td>WANG Yu, WANG Beining, LIN Pingping, LIU Dongming</td> 
 +
</tr>
  
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">BBa_K1689007</a></td>
 +
<td>Composite</td>
 +
<td>dCas9-C-luc</td>
 +
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 +
</tr>
  
−
<h4>Inspiration</h4>
+
<tr>  
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
  <td>&nbsp;</td>
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">BBa_K1689008</a></td>
 +
<td>Composite</td>
 +
<td>dCas9-N-luc</td>
 +
<td>LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping</td>
 +
</tr>  
  
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
+
<tr>
−
<ul>
+
<td>&nbsp;</td>
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
+
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">BBa_K1689011</a></td>  
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
+
<td>Composite</td>  
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
+
<td>dCas9-delta alpha</td>
−
</ul>
+
<td>ZHANG Yihao, WEI Weijia</td>  
 +
</tr>  
  
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">BBa_K1689012</a></td>
 +
<td>Composite</td>
 +
<td>dCas9-Nlact</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
  
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013" style="color:#00afd1">BBa_K1689013</a></td>
 +
<td>Compostie</td>
 +
<td>Nlact-dCas9</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
  
−
<h4>Part Table </h4>
+
<tr>
−
</html>
+
<td>&nbsp;</td> 
−
<groupparts>iGEM015 Example</groupparts>
+
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">BBa_K1689014</a></td>  
−
<html>
+
<td>Coding</td>  
 +
<td>dCas9-Clact</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>  
  
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">BBa_K1689015</a></td>
 +
<td>Coding</td>
 +
<td>F[1,2]-dCas9</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
  
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">BBa_K1689016</a></td>
 +
<td>Coding</td>
 +
<td>dCas9-F[1,2]</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
  
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">BBa_K1689017</a></td>
 +
<td>Coding</td>
 +
<td>F3-dCas9</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">BBa_K1689018</a></td>
 +
<td>Coding</td>
 +
<td>dCas9-F3</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">BBa_K1689019</a></td>
 +
<td>Coding</td>
 +
<td>delta alpha-dCas9</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
 +
<tr>
 +
<td>&nbsp;</td> 
 +
<td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">BBa_K1689020</a></td>
 +
<td>Coding</td>
 +
<td>dCas9-delta omega</td>
 +
<td>ZHANG Yihao, WEI Weijia</td>
 +
</tr>
 +
</table>
 
</div>
 
</div>
 +
 +
                <p><b>Parts for paired dCas9 reporter system</b></p>
 +
 +
                <p>Nluc416-FRB <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689003" style="color:#00afd1">(BBa_K1689003)</a>,<br/> FKBP-Cluc398 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689005" style="color:#00afd1">(BBa_K1689005)</a>,<br/> Nluc398-FRB<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689004" style="color:#00afd1"> (BBa_K1689004)</a>, <br/>FKBP-Cluc394<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689006" style="color:#00afd1"> (BBa_K1689006)</a><br/> are used to validate the reconsitituation activity of split luciferase.</p>
 +
                <p>Nluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a>,<br/> Cluc-dCas9<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a>,<br/> dCas9-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">(BBa_K1689008)</a>,<br/> dCas9-Cluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689007)</a><br/> fusion proteins are used for constructing paired dCas9 reporter system.</p>
 +
                <p>&Delta;α-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689011)</a>,<br/> dCas9-Nlact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">(BBa_K1689012)</a>,<br/> Nlact-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689013)</a>,<br/> dCas9-Clact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689014)</a>,<br/> F[1,2]-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">(BBa_K1689015)</a>,<br/> dCas9-F[1,2] <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">(BBa_K1689016)</a>, <br/>F3-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689017" style="color:#00afd1">(BBa_K1689017)</a>,<br/>  dCas9-F3 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">(BBa_K1689018)</a>,<br/> dCas9-&Delta;&alpha;<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1"> (BBa_K1689019)</a>,<br/> &Delta;&omega;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a><br/> are used for further development of dectection.</p>
 +
                <p><b>Parts for Molecular Beacon</b></p>
 +
                <p>STV-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689001" style="color:#00afd1">(BBa_K1689001)</a>,<br/> Cluc-STV <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689002" style="color:#00afd1">(BBa_K1689002)</a><br/> are used for binding to molecular beacon.</p>
 +
</div>
 +
                <p id="Collection"><b>Parts for sgRNA generation</b></p>
 +
<div>
 +
                <p>sgRNA generator <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689000" style="color:#00afd1">(BBa_K1689000)</a> is used for generating sgRNA</p>
 +
             
 +
</div>
 +
                <h3 class="classic-title" style="margin-top:50px"><span>Part Collection</span></h3>
 +
<div>
 +
                <p>2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explored a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the  form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.</p>
 +
                <p>We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".</p>
 +
                <p>The Collection includes:</p>
 +
 +
                <p><b>dCas9-split luciferase Parts</b></p>
 +
                <p>Nluc-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689010" style="color:#00afd1">(BBa_K1689010)</a><br/>
 +
                Cluc-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689009" style="color:#00afd1">(BBa_K1689009)</a><br/>
 +
                dCas9-Nluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689008" style="color:#00afd1">(BBa_K1689008)</a><br/>
 +
                dCas9-Cluc <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689007" style="color:#00afd1">(BBa_K1689007)</a></p>
 +
 +
                <p><b>dCas9-split Dihydrofolate Reductase Parts</b></p>
 +
                <p>F[1,2]-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689015" style="color:#00afd1">(BBa_K1689015)</a><br/>
 +
                F3-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689017)</a><br/>
 +
                dCas9-F[1,2] <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689016" style="color:#00afd1">(BBa_K1689016)</a><br/>
 +
                dCas9-F3 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689018" style="color:#00afd1">(BBa_K1689018)</a></p>
 +
 +
                <p><b>dCas9-split β–Lactamase Parts</b></p>
 +
                <p>dCas9-Nlact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689012" style="color:#00afd1">(BBa_K1689012)</a><br/>
 +
                Nlact-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689013" style="color:#00afd1">(BBa_K1689013)</a><br/>
 +
                dCas9-Clact <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689014" style="color:#00afd1">(BBa_K1689014)</a></p>
 +
               
 +
                <p><b>dCas9-splitβ–Galactosidase Parts</b></p>
 +
                <p>&Delta;&alpha;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689011" style="color:#00afd1">(BBa_K1689011)</a><br/>
 +
                dCas9-&Delta;&alpha; <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689019" style="color:#00afd1">(BBa_K1689019)</a><br/>
 +
                &Delta;&omega;-dCas9 <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1689020" style="color:#00afd1">(BBa_K1689020)</a></p>
 +
                <p>Among them, the PC reporters with split luciferase oxidizes luciferin and gives out bioluminescence signal, while the other three reporters take electric signal as their output, which is also able to be detected and qualified.</p>
 +
                </div>
 +
 +
                </div>
 +
            </div>
 +
          </div>
 +
        </div>
 +
      </div>
 +
    </div>
 +
  </div>
 +
</div>
 +
 +
 +
 +
 +
                </div>
 +
 +
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Latest revision as of 00:40, 20 November 2015

Peking iGEM 2015

Parts

Each part gives us a new experience.

Favorite Parts

Nluc-dCas9 fusion protein
(BBa_K1689010)

Nluc was fused to the N terminus of dCas9 to form the Nluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Cluc-dCas9 fusion protein (BBa_K1689009) and sgRNAs, our paired dCas9 reporter system were constructed.

Cluc-dCas9 fusion protein
(BBa_K1689009)

Cluc was fused to the N terminus of dCas9 to form the Cluc-dCas9 fusion protein. It is used to bind a DNA sequence guided by sgRNA. Paired with Nluc-dCas9 fusion protein (BBa_K1689010) and sgRNAs, our paired dCas9 reporter system were constructed.

sgRNA generator
(BBa_K1689000)

sgRNA generator is used for generating sgRNA which recognizes targeted DNA, then guiding dCas9 to it.

Part List

Favorite Name Type Description Design
 √ BBa_K1689000 Composite sgRNA generator LIU Wenchao, XU Luze
 √ BBa_K1689009 Composite C-luc-dCas9 LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
 √ BBa_K1689010 Compostie N-luc-dCas9 LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
  BBa_K1689001 Coding Coding sequence of STV-N-luc LI Xiang, LI Jiaofeng
  BBa_K1689002 Coding Coding sequence of Cluc-STV LI Xiang, LI Jiaofeng
  BBa_K1689003 Coding Coding sequence of Nluc416-FRB WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689004 Coding Coding sequence of Nluc398-FRB WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689005 Coding Coding sequence of FKBP-Cluc398 WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689006 Coding Coding sequence of FKBP-Cluc394 WANG Yu, WANG Beining, LIN Pingping, LIU Dongming
  BBa_K1689007 Composite dCas9-C-luc LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
  BBa_K1689008 Composite dCas9-N-luc LIU Wenchao, WANG Yu, WANG Beining, LIN Pingping
  BBa_K1689011 Composite dCas9-delta alpha ZHANG Yihao, WEI Weijia
  BBa_K1689012 Composite dCas9-Nlact ZHANG Yihao, WEI Weijia
  BBa_K1689013 Compostie Nlact-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689014 Coding dCas9-Clact ZHANG Yihao, WEI Weijia
  BBa_K1689015 Coding F[1,2]-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689016 Coding dCas9-F[1,2] ZHANG Yihao, WEI Weijia
  BBa_K1689017 Coding F3-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689018 Coding dCas9-F3 ZHANG Yihao, WEI Weijia
  BBa_K1689019 Coding delta alpha-dCas9 ZHANG Yihao, WEI Weijia
  BBa_K1689020 Coding dCas9-delta omega ZHANG Yihao, WEI Weijia

Parts for paired dCas9 reporter system

Nluc416-FRB (BBa_K1689003),
FKBP-Cluc398 (BBa_K1689005),
Nluc398-FRB (BBa_K1689004),
FKBP-Cluc394 (BBa_K1689006)
are used to validate the reconsitituation activity of split luciferase.

Nluc-dCas9(BBa_K1689010),
Cluc-dCas9(BBa_K1689009),
dCas9-Nluc (BBa_K1689008),
dCas9-Cluc (BBa_K1689007)
fusion proteins are used for constructing paired dCas9 reporter system.

Δα-dCas9 (BBa_K1689011),
dCas9-Nlact (BBa_K1689012),
Nlact-dCas9 (BBa_K1689013),
dCas9-Clact (BBa_K1689014),
F[1,2]-dCas9 (BBa_K1689015),
dCas9-F[1,2] (BBa_K1689016),
F3-dCas9 (BBa_K1689017),
dCas9-F3 (BBa_K1689018),
dCas9-Δα (BBa_K1689019),
Δω-dCas9 (BBa_K1689020)
are used for further development of dectection.

Parts for Molecular Beacon

STV-Nluc (BBa_K1689001),
Cluc-STV (BBa_K1689002)
are used for binding to molecular beacon.

Parts for sgRNA generation

sgRNA generator (BBa_K1689000) is used for generating sgRNA

Part Collection

2015 Peking iGEM has not only transformed the sequence information into detectable bioluminescence, but also explored a wide range in which Paired dCas9 (PC) Reporter System is able to be applied. Since we have chosen split enzyme as our reporter, it can be substituted by various kinds of enzymes, thus the form of read-out will be abundant at the same time. That is, our PC reporter system has successfully provided a platform to produce a variety of signals.

We combine the specific sequence binding activity of dCas9 with diverse characteristics of split enzymes, thus creating a part collection named "PC Reporters Collection".

The Collection includes:

dCas9-split luciferase Parts

Nluc-dCas9 (BBa_K1689010)
Cluc-dCas9 (BBa_K1689009)
dCas9-Nluc (BBa_K1689008)
dCas9-Cluc (BBa_K1689007)

dCas9-split Dihydrofolate Reductase Parts

F[1,2]-dCas9 (BBa_K1689015)
F3-dCas9 (BBa_K1689017)
dCas9-F[1,2] (BBa_K1689016)
dCas9-F3 (BBa_K1689018)

dCas9-split β–Lactamase Parts

dCas9-Nlact (BBa_K1689012)
Nlact-dCas9 (BBa_K1689013)
dCas9-Clact (BBa_K1689014)

dCas9-splitβ–Galactosidase Parts

Δα-dCas9 (BBa_K1689011)
dCas9-Δα (BBa_K1689019)
Δω-dCas9 (BBa_K1689020)

Among them, the PC reporters with split luciferase oxidizes luciferin and gives out bioluminescence signal, while the other three reporters take electric signal as their output, which is also able to be detected and qualified.