Difference between revisions of "Team:SVA-NYC/Chemical-Parts"

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<h2>Chemical Parts</h2>
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<h1>Chemical Parts</h1>
  
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<h2>LaMotte Kit Model STH Series - Combination Soil Testing Outfits (product ID #5029) Reagents:</h2>
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<p>
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  <img src="https://static.igem.org/mediawiki/2015/8/80/Test_svg.svg" alt="test SVG file"  width="90%" height="90%" />
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Soil Flocculating Reagent (5643WT)
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  </div>
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Universal Extracting Solution (5173)
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Duplex Indicator (2221)
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Bromcresol Green (2207)
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Chlorphenol Red (2209)
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Bromthymol Blue (2210)
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Phenol Red (2211)
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Thymol Blue (2213)
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Potassium Reagent B Tablets (5161A)
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Potassium Reagent C (5162)
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Phosphorus Reagent 2 (5156)
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Phosphorus Test Tablets (5706A)
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Nitrate Reagent #1 (5146)
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Nitrate Reagent #2 Powder (5147)
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Humus Screening Reagent Powder (5119)
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Calcium Test Solution (5108PS)
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Ammonia Nitrogen Test Solution (5103PS)
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Magnesium Test Solution 1 (5140)
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Magnesium and Manganese Test Solution #2 (5145WT)
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Manganese Periodate Reagent (6311)
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Aluminum Test Solution (5101)
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Nitrite Nitrogen Reagent 1 (5151WT)
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Nitrite Nitrogen Reagent 2 (5152WT)
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Nitrite Nitrogen Reagent 3 (5153WT)
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Ferric Iron Test Solution (5116PS)
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Sulfate Test Solution (5171)
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Chloride Test Solution (5111)
  
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<hr>
  
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    <p>A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
 
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  <p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
 
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<h2>Standards:</h2>
  
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<p>
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Chloride standard from NaCl
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Total M.W.= 58.44
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Cl M.W.= 35.5
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500ppm = .014084507 mol =
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0.20577469 grams in 250 mL
  
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<hr>
  
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Potassium standard from KCl
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Total M.W.= 74.55
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K M.W.= 39.0983
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400ppm = .0028139 mol =
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0.052 grams in 250 mL
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<hr>
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Sulfate standard from MgSO4 * 7H2O
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Total M.W.= 246.47
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SO4 M.W.= 96.06
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2000 ppm = .02082032 mol =
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1.28289611 grams in 250 mL
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<hr>
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Nitrite standard from NaNO2
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Total M.W.= NaNO2 68.9953
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NO2 M.W.= 46.01
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50 ppm = .00108672 mol =
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.01874465 grams in 250 mL
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<hr>
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Nitrate standard from KNO3
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Total M.W.= 101.1
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NO3 M.W.= 62.0049
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75 ppm = .00120958 mol =
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.03057218 grams in 250 mL
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<hr>
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Calcium standard from CaCl2
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Total M.W.= 110.98
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Ca M.W.= 40.98
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2800 ppm = .06832601 mol
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1.89570522 grams in 250 mL
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<hr>
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Aluminum standard from AlK(SO4)2 * 12H20
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Total M.W.= 474.39
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Al M.W.= 26.981538
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125 ppm = .0046328 mol =
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.54943819 grams in 250 mL
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<hr>
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Manganese standard from MnSO4*H2O
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Total M.W.= 169.01
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Mn M.W.= 54.938044
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molar ratio is 0.32505795
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40 ppm = 0.00023667(total) = 0.00007693 (Mn)
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0.00325055g in 250mL
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0.650g in 50mL = 1000x
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250uL of 1000x in 250mL = 1x 40ppm Mn Standard
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<hr>
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Phosphate standard from KH2PO4
 +
Total M.W.= 136.09
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P M.W.= 30.973762
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200 ppm = .00645708 =
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.21968594 grams in 250 mL
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<hr>
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Ammonia standard from NH4Cl
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Total M.W.= 53.49
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NH3 M.W.= 17.031
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150 ppm = .00880747 mol =
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.11777788 grams in 250 mL
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<hr>
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Magnesium Standard from MgCl2 * 6H2O
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Total M.W.= 203.3
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Mg M.W.= 24.305
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150 ppm = .00617157 mol =
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.31367003 grams in 250 mL
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<hr>
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Ferric Iron standard from Fe2(SO4)3 * 9H2O
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Total M.W.= 562
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Fe M.W.= 55.845
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62.5 ppm = .00055958 mol =
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.07862163 grams in 250 mL
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Revision as of 03:10, 19 September 2015


soiled

SVA-NYC

Chemical Parts

LaMotte Kit Model STH Series - Combination Soil Testing Outfits (product ID #5029) Reagents:

Soil Flocculating Reagent (5643WT) Universal Extracting Solution (5173) Duplex Indicator (2221) Bromcresol Green (2207) Chlorphenol Red (2209) Bromthymol Blue (2210) Phenol Red (2211) Thymol Blue (2213) Potassium Reagent B Tablets (5161A) Potassium Reagent C (5162) Phosphorus Reagent 2 (5156) Phosphorus Test Tablets (5706A) Nitrate Reagent #1 (5146) Nitrate Reagent #2 Powder (5147) Humus Screening Reagent Powder (5119) Calcium Test Solution (5108PS) Ammonia Nitrogen Test Solution (5103PS) Magnesium Test Solution 1 (5140) Magnesium and Manganese Test Solution #2 (5145WT) Manganese Periodate Reagent (6311) Aluminum Test Solution (5101) Nitrite Nitrogen Reagent 1 (5151WT) Nitrite Nitrogen Reagent 2 (5152WT) Nitrite Nitrogen Reagent 3 (5153WT) Ferric Iron Test Solution (5116PS) Sulfate Test Solution (5171) Chloride Test Solution (5111)

Standards:

Chloride standard from NaCl Total M.W.= 58.44 Cl M.W.= 35.5 500ppm = .014084507 mol = 0.20577469 grams in 250 mL

Potassium standard from KCl Total M.W.= 74.55 K M.W.= 39.0983 400ppm = .0028139 mol = 0.052 grams in 250 mL
Sulfate standard from MgSO4 * 7H2O Total M.W.= 246.47 SO4 M.W.= 96.06 2000 ppm = .02082032 mol = 1.28289611 grams in 250 mL
Nitrite standard from NaNO2 Total M.W.= NaNO2 68.9953 NO2 M.W.= 46.01 50 ppm = .00108672 mol = .01874465 grams in 250 mL
Nitrate standard from KNO3 Total M.W.= 101.1 NO3 M.W.= 62.0049 75 ppm = .00120958 mol = .03057218 grams in 250 mL
Calcium standard from CaCl2 Total M.W.= 110.98 Ca M.W.= 40.98 2800 ppm = .06832601 mol 1.89570522 grams in 250 mL
Aluminum standard from AlK(SO4)2 * 12H20 Total M.W.= 474.39 Al M.W.= 26.981538 125 ppm = .0046328 mol = .54943819 grams in 250 mL
Manganese standard from MnSO4*H2O Total M.W.= 169.01 Mn M.W.= 54.938044 molar ratio is 0.32505795 40 ppm = 0.00023667(total) = 0.00007693 (Mn) 0.00325055g in 250mL 0.650g in 50mL = 1000x 250uL of 1000x in 250mL = 1x 40ppm Mn Standard
Phosphate standard from KH2PO4 Total M.W.= 136.09 P M.W.= 30.973762 200 ppm = .00645708 = .21968594 grams in 250 mL
Ammonia standard from NH4Cl Total M.W.= 53.49 NH3 M.W.= 17.031 150 ppm = .00880747 mol = .11777788 grams in 250 mL
Magnesium Standard from MgCl2 * 6H2O Total M.W.= 203.3 Mg M.W.= 24.305 150 ppm = .00617157 mol = .31367003 grams in 250 mL
Ferric Iron standard from Fe2(SO4)3 * 9H2O Total M.W.= 562 Fe M.W.= 55.845 62.5 ppm = .00055958 mol = .07862163 grams in 250 mL

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SVA Bio Art Lab
School of Visual Arts

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