Difference between revisions of "Team:SVCE Chennai/Project"
Abrahamrkj (Talk | contribs) |
Abrahamrkj (Talk | contribs) |
||
| Line 145: | Line 145: | ||
plasmids, we modulated our design to a plasmid with two different expression cassettes.</p> | plasmids, we modulated our design to a plasmid with two different expression cassettes.</p> | ||
| + | <p>The two cassettes are under the regulation of different promoters for the expression of the | ||
| + | |||
| + | different phenotypes. Minicell phenotype, driven by the overexpression of the ftsZ gene, is under | ||
| + | |||
| + | the regulation of the IPTG-controlled lac promoter. Bacteriocin production is driven by the | ||
| + | |||
| + | arabinose operon.</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/thumb/b/ba/Plasmid_design_v1.jpg/652px-Plasmid_design_v1.jpg" class="img-responsive"> | ||
| + | |||
| + | <p> | ||
| + | In order to confirm the expression of ftsZ, GFP is included under the lac promoter control by the | ||
| + | |||
| + | introduction of an RBS after the ftsZ stop codon. Similarly, bacteriocin production is tagged to | ||
| + | |||
| + | RFP. | ||
| + | </p> | ||
| + | |||
| + | <img src="https://static.igem.org/mediawiki/2015/thumb/b/ba/Plasmid_design_v1.jpg/652px-Plasmid_design_v1.jpg" class="img-responsive"> | ||
| + | |||
</div> | </div> | ||
Revision as of 19:34, 18 September 2015
Project: Minicins
Project Description
Our project aims to neuter multidrug resistant bacterial infections with the help of bacteriocins in a minicell chassis.
Firstly, the idea was to use two different plasmids – one for minicell division and the other for bacteriocin production. Then, we realized that plasmids are carried to the minicells. This requires the use of two different plasmids with two different promoters. Instead of going for two different plasmids, we modulated our design to a plasmid with two different expression cassettes.
The two cassettes are under the regulation of different promoters for the expression of the different phenotypes. Minicell phenotype, driven by the overexpression of the ftsZ gene, is under the regulation of the IPTG-controlled lac promoter. Bacteriocin production is driven by the arabinose operon.
In order to confirm the expression of ftsZ, GFP is included under the lac promoter control by the introduction of an RBS after the ftsZ stop codon. Similarly, bacteriocin production is tagged to RFP.