Team:SVCE Chennai/Notebook


APRIL 2015

  • Recruitment of team members
  • Setting up of lab with necessary equipments and chemicals
  • Brainstorming


  • Brainstorming
  • University exams hence no lab work


  • lab practice sessions
  • confirmation of project title


Week 1

  • Submitted safety forms and also regarding our project forms.
  • Preparation of LB broth and LB Agar and plates were poured.
  • Stab culture for FtsZ was obtained from iGEM and was streaked on chloramphenicol LB plates.
  • Abstract and title of our project was uploaded.
  • Prepared chemicals and buffers for plasmid isolation.
  • FtsZ was innoculated in LB broth.
  • Pure culture of Pseudomonas aeruginosa and BL21 strain was obtained.
  • Sequences for gBlocks were constructed for ordering.

Week 2:

  • Plasmid isolation was performed for protocol optimisation.
  • Result was negative and so the isolation was performed again.
  • Elution of DNA was done but results were not satisfactory.
  • Media (SOB and SOC) were prepared for competent cell preparation.
  • Positive results were obtained for elution of DNA and protocol was optimised.
  • Innoculated BL 21 in SOB.
  • Presentation and questionnaire based on our project was prepared to educate the students in different colleges.
  • Logo designing was initiated.

Week 3:

  • Growth curve for Pseudomonas aeruginosa was done and results were analysed.
  • Growth curve for BL 21 was done.
  • Plasmid isolation of pET24a was performed.
  • Competent cell of BL 21 was made and protocol was optimised.
  • Seed stock for BL 21 competent cell was made.
  • Transformation was performed.
  • Primers required for our project were designed and ordered.
  • For Human Practise questionnaire for hospitals were prepared.
  • Presentation was done in Arulmigu Meenakshi Amman College and in Prathyusha Institute of Technology and Management.
  • Dr. Kannan from Saveetha Medical College was interviewed regarding antibiotic resistance.
  • Logo design was reviewed and changes were made.
  • Approached various funding agencies.
  • Collaboration with Uppsala was confirmed.

Week 4:

  • BL21 was subcultured.
  • Transformation efficiency of pET 24a in E coli BL21 competent cells was calculated.
  • Chloramphenicol plates were prepared for pSB1C3 amplification.
  • Methicillin Resistant Staphylococcus aureus were obtained.
  • Final safety forms were filled.
  • First step was taken for wiki page and its theme was discussed.
  • Responses for various questionnaire was collected and a statistical analysis was carried out.
  • Results were presented to the faculty members of our department.
  • Three doctors from reputed medical institutions were interviewed regarding the development of antibiotic resistance and formation of biofilms in hospital environments.


Week 1:

  • gBlocks of ftsZ was obtained.
  • PCR amplification of the ftsZ was done.
  • Results were found to be negative.
  • Hence they were troubleshooted.
  • Biochemical tests were performed for Staphylococcus and Pseudomonas. A presentation was done during the national level symposium called OMICS and created an awareness among various other college students.

Week 2:

  • gBlocks of thuricin was obtained.
  • PCR amplification of the gene was done.
  • This amplified gene was cloned into pSB1C3.
  • Growth curve for Staphylococcus aureus was done.
  • Human practices team presented in Madha Engineering College and SBOA.

Week 3:

  • Got bactofencin gBlocks and PCR amplification was done.
  • Then cloning was done in pSB1C3.
  • Cloned Thuricin and Bactofencin were submitted as parts.
  • Public interview regarding antibiotic resistance was taken.
  • Descriptions were uploaded in wiki page.
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Sri Venkateswara College of Engineering, Chennai