Team:Stockholm/Attributions

Attributions

All experiments conducted in this project has been designed, executed and analysed by members of the iGEM Group Stockholm. We, as iGEM Team Stockholm, are confidently standing behind the presented data. We have put a special focus on not altering the actual picture, we got by conducting our experiments.

At this point, we want to acknowledge the work of Roger Draheim, who was the only one throughout the project who has been conducting similar experiments from us independently which we later compared. Among these experiments, you can find the prediction of EnvZ (extracellular domain) and the characterization of the EnvZ gene in his expression vector from which we derived our EnvZ biobrick (BBa_17-----). In both given example, we got to similar results and can therefore give a confident conclusion to these results presented.

Our project has been developed inpendently by other projects. We are not really building up on a single project. We discovered that the iGEM Team from Dundee 2013 has been working with a chimeric version of EnvZ as well. Therefore, we have analysed their project and adjusted our experiments accordingly. After request, we were also very happy to receive the EnvZ deficient E.Coli strain BW25113 from Dundee, in person of Mr._________. We have been also inspired by the work that has been already conducted with the OmpR signaling cascade. Notably, the former iGEM Teams from Technion Israel.

Supervisors

Johan Rockberg, KTH Royal Institute of Technology
Ute Römling, Karolinska Institutet
Hakan Jönsson, KTH Royal Institute of Technology
Teresa Frisan, Karolinska Institutet

At this point, we would like to thank our supervisors who have been accompanying us throughout the entire project. They have been feedbacking us in weekly meetings, given us new ideas and promoted our research. We are glad of their engagement for this student-driven research project and that they have been helping us not only scientifically but also administratively to get insurance and a lab in which we have been performing our research in.

Others

Roger Draheim, University of Portsmouth represents our unofficial supervisor. He has been formerly working at the Stockholm University and is an expert in the field of Osmoregulation via EnvZ-OmpR pathways. He has provided us with the MDG147 and EPB30 strains which have a genomically integrated YFP/CFP reporter system depending on the phosphorylation status of OmpR. We would like to thank Roger for his advice, feedback and constructive suggestions throughout the entire project. We can’t thank you enough.

Gunnar von Heijne, Stockholm University has provided us with many good contacts (among which we find Roger Draheim) and has helped us to get the answers we needed from experts in the field. His kind and immediate reactions to questions related to our project has been very much acknowledged.

Martin Högbom, Stockholm University is a structural biologist and has been supporting our project as we were creating our own chimeric constructs. He gave us an insight in the possibilities of protein folding and the strengths and weaknesses for structure predictions.
Jan Willem De Gier, Stockholm University supported our project by giving us strong advice in questions related to surface protein expression. We furthermore could discuss with him the possibility of using spheroplasts for testing gene expression after our system. He kindly provided us with a protocol for spheroplasting E.Coli strains.
Tae Hyun Kang, The University of Texas Austin is a PhD who has been supporting our project by sending us a protocol for the production of spheroplasts from E.Coli. We are very glad about our correspondance with him and for all the answers to our several questions.
Francis Jingxin Hu, KTH Royal Institute of Technology Stockholm is a PhD student from the laboratory of Johan Rockberg and he has been showing us different microbiological techniques to start our project with.
Niklas Thalén, KTH Royal Institute of Technology Stockholm working mainly with fluorescent agents. He is another PhD student of the laboratory of Johan Rockberg and has been introducing us to the plate reader we could use and has been furthermore providing us with more information related to fluorescent measurements.
Ken Andersson, KTH Royal Institute of Technology Stockholm has provided us with advice and suggestions in the laboratory. He helped us producing big amounts of competent cells and has been, besides his work as PhD student, always willing to help us in our project.
Matheus Dyczynski, Karolinska Institutet Solna is a Post-Doc who has been working with different cloning and transformation methods. We would like to thank Matheus for giving us a seminar about cloning and transformation methods and to be a contact for cloning related questions.
Jan-Olof Höög, Karolinska Institutet Solna has supported this group from the very beginning before the teams was yet formed or registered. He helped to pass administrational barriers and supported the team with the right contacts and short cuts to make it to this years competition.
Lars-Arne Haldosen, Karolinska Institutet Solna represents first supporters of this year’s iGEM team. He helped to make the participation in this competition popular and supported our project throughout the entire process of registration, insurance and laboratory search.
Anders Andersson, KTH Royal Institute of Technology Stockholm is the most influential “close-to-be” supervisor. He has supported the project with new ideas and advice in its founding phase and provided us with new insights. Unfortunately, he had to drop out of the project, however we thank you for your kind discussions.
Hans-Georg Koch, University Freiburg has been providing us with a protocol for subcellular fragmentation, which we haven’t been able to use during the short time of our project, but we would like to acknowledge his spirit and kindness to provide the protocol.
Annika Cimdins, Karolinska Institutet Solna, has been supervising our experiments in the very last weeks of the project in which we were working in the laboratory of our supervisor Ute Römling.