Difference between revisions of "Team:Stockholm/Protocols"
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<a href="https://static.igem.org/mediawiki/2015/7/7b/STHLM_Spheroplast_protocol.pdf" >Spheroplast </a> | <a href="https://static.igem.org/mediawiki/2015/7/7b/STHLM_Spheroplast_protocol.pdf" >Spheroplast </a> | ||
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| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2015/e/e3/STHLM_FACS_protocol.pdf" >FACS</a> |
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<a href="https://static.igem.org/mediawiki/2015/8/86/STHLM_Affinity_separation_protocol.pdf" >Affinity separation (unfinished) </a> | <a href="https://static.igem.org/mediawiki/2015/8/86/STHLM_Affinity_separation_protocol.pdf" >Affinity separation (unfinished) </a> | ||
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<a href="https://static.igem.org/mediawiki/2015/1/17/STHLM_DotBlot_protocol.pdf" >Dot blot </a> | <a href="https://static.igem.org/mediawiki/2015/1/17/STHLM_DotBlot_protocol.pdf" >Dot blot </a> | ||
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| − | <a href="https://static.igem.org/mediawiki/2015/ | + | <a href="https://static.igem.org/mediawiki/2015/6/68/STHLM_ELISA_new_protocol.pdf" >ELISA</a> |
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<a href="https://static.igem.org/mediawiki/2015/0/05/STHLM_Alternative_Osmolarity_protocol.pdf" >Alternative osmolarity</a> | <a href="https://static.igem.org/mediawiki/2015/0/05/STHLM_Alternative_Osmolarity_protocol.pdf" >Alternative osmolarity</a> | ||
Revision as of 08:46, 18 September 2015
Protocols
Western blot
Membrane stripping
Spheroplast
FACS
Affinity separation (unfinished)
Dot blot
ELISA
Alternative osmolarity
Overlap PCR
Transformation
Colony PCR
Osmolarity
Site-directed mutagenesis
AHL osmolarity dependence
3A assembly
Chemically competent cells
Overnight cultures and restriction analysis
Traditional cloning (Restriction cloning)