Difference between revisions of "Team:Tec-Chihuahua/Notebook"
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| − | + | <!-- START OF THE JOURNAL!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! --> | |
<h1 id="journal" class="arrow">Journal</h1> | <h1 id="journal" class="arrow">Journal</h1> | ||
| − | + | ||
| − | + | ||
| − | + | ||
<div class="col-md-12"> | <div class="col-md-12"> | ||
<div class="col-md-6 journal-left"> | <div class="col-md-6 journal-left"> | ||
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</div> | </div> | ||
</div> | </div> | ||
| − | |||
<div class="col-md-12"> | <div class="col-md-12"> | ||
<div class="col-md-6 col-md-offset-6 journal-right"> | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
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<div class="col-md-12 in-quote"> | <div class="col-md-12 in-quote"> | ||
<p>Team promotional photo.</p> | <p>Team promotional photo.</p> | ||
| − | <img src=" | + | <img src="https://static.igem.org/mediawiki/2015/7/7c/Tec-chihuahua-notebook-march04.jpeg"> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | |||
<div class="col-md-12"> | <div class="col-md-12"> | ||
<div class="col-md-6 col-md-offset-6 journal-right"> | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
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<div class="col-md-12 in-quote"> | <div class="col-md-12 in-quote"> | ||
<p>We presented our ideas to experts of the Centro de Investigación en Materiales Avanzados (CIMAV), they seem interested on the Project and gave us their support. </p> | <p>We presented our ideas to experts of the Centro de Investigación en Materiales Avanzados (CIMAV), they seem interested on the Project and gave us their support. </p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/f/fa/Tec-chihuahua-notebook-abril09.jpeg" > | |
</div> | </div> | ||
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<div class="col-md-12 in-quote"> | <div class="col-md-12 in-quote"> | ||
<p>We went to the local TV Show “Emma de la O” to talk about the biotechnology and our project.</p> | <p>We went to the local TV Show “Emma de la O” to talk about the biotechnology and our project.</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/d/d8/Tec-chihuahua-notebook-abril17.jpeg"> | |
</div> | </div> | ||
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</div> | </div> | ||
</div> | </div> | ||
| − | |||
<div class="col-md-12"> | <div class="col-md-12"> | ||
<div class="col-md-6 col-md-offset-6 journal-right"> | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
| Line 233: | Line 228: | ||
<div class="col-md-12 in-quote"> | <div class="col-md-12 in-quote"> | ||
<p>Purification of the NP-CNT</p> | <p>Purification of the NP-CNT</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/e/e4/Tec-chihuahua-notebook-may12.jpeg"> | |
</div> | </div> | ||
| Line 266: | Line 261: | ||
<p>We finished the filtration of the nanotubes</p> | <p>We finished the filtration of the nanotubes</p> | ||
<p>Plasmid extraction of <span class="bacteria">E. coli</span></p> | <p>Plasmid extraction of <span class="bacteria">E. coli</span></p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/9/9d/Tec-chihuahua-notebook-may22.jpeg"> | |
| − | + | ||
</div> | </div> | ||
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<div class="col-md-12 in-quote"> | <div class="col-md-12 in-quote"> | ||
<p>We measured the purity of plasmid using the NanoDrop 2000</p> | <p>We measured the purity of plasmid using the NanoDrop 2000</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/8/8f/Tec-chihuahua-notebook-may25.jpeg"> | |
</div> | </div> | ||
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</div> | </div> | ||
</div> | </div> | ||
| − | |||
<div class="col-md-12"> | <div class="col-md-12"> | ||
<div class="col-md-6 journal-left"> | <div class="col-md-6 journal-left"> | ||
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<p>We finished drying the nanotubes.</p> | <p>We finished drying the nanotubes.</p> | ||
<p>Preparation of PBS</p> | <p>Preparation of PBS</p> | ||
| − | |||
</div> | </div> | ||
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<div class="col-md-12 in-quote"> | <div class="col-md-12 in-quote"> | ||
<p>We began the work with the bovine oocytes at the Facultad de Zootecnia (School of Animal Husbandry) of the Universidad Autónoma de Chihuahua (UACH).</p> | <p>We began the work with the bovine oocytes at the Facultad de Zootecnia (School of Animal Husbandry) of the Universidad Autónoma de Chihuahua (UACH).</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/4/44/Tec-chihuahua-notebook-june08.jpeg"> | |
| − | + | ||
</div> | </div> | ||
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<p>Oocytes extraction and selection.</p> | <p>Oocytes extraction and selection.</p> | ||
<p>The maduration of the viables oocytes began.</p> | <p>The maduration of the viables oocytes began.</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/0/01/Tec-chihuahua-notebook-june09.jpeg"> | |
</div> | </div> | ||
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<p>We began the CNT-Fluorescein functionalization.</p> | <p>We began the CNT-Fluorescein functionalization.</p> | ||
<p>Cleaning and picking of the oocytes.</p> | <p>Cleaning and picking of the oocytes.</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/1/13/Tec-chihuahua-notebook-june10.jpeg"> | |
</div> | </div> | ||
| Line 374: | Line 366: | ||
<div class="col-md-12 in-quote"> | <div class="col-md-12 in-quote"> | ||
<p>We finished the CNT functionalization with fluorescein.</p> | <p>We finished the CNT functionalization with fluorescein.</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/3/3c/Tec-chihuahua-notebook-june11.jpeg"> | |
| − | + | ||
</div> | </div> | ||
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<div class="col-md-12 in-quote"> | <div class="col-md-12 in-quote"> | ||
<p>Pins Ceremony</p> | <p>Pins Ceremony</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/d/dc/Tec-chihuahua-notebook-june15.jpeg"> | |
</div> | </div> | ||
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<div class="col-md-12 in-quote"> | <div class="col-md-12 in-quote"> | ||
<p>Preparation of the stocks and the media for the handling and maduration of oocytes.</p> | <p>Preparation of the stocks and the media for the handling and maduration of oocytes.</p> | ||
| − | |||
| − | |||
</div> | </div> | ||
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<p>Oocytes extraction and selection.</P> | <p>Oocytes extraction and selection.</P> | ||
<p>The maduration of the viables oocytes began.</p> | <p>The maduration of the viables oocytes began.</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/a/a7/Tec-chihuahua-notebook-june24.jpeg"> | |
</div> | </div> | ||
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<p>F-NTC / Oocytes interaction.</p> | <p>F-NTC / Oocytes interaction.</p> | ||
<p>Filtration of the second group of nanotubes</p> | <p>Filtration of the second group of nanotubes</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/3/3d/Tec-chihuahua-notebook-june25.jpeg"> | |
| − | + | ||
</div> | </div> | ||
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<p>Team meeting.</p> | <p>Team meeting.</p> | ||
<p>Observation of functionalized oocytes with confocal microscope.</p> | <p>Observation of functionalized oocytes with confocal microscope.</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/c/c5/Tec-chihuahua-notebook-june29.jpeg"> | |
</div> | </div> | ||
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<div class="col-md-12 in-quote"> | <div class="col-md-12 in-quote"> | ||
<p>Filtration of the CNT.</p> | <p>Filtration of the CNT.</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/2/25/Tec-chihuahua-notebook-june30.jpeg"> | |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | |||
| − | |||
<div class="col-md-12"> | <div class="col-md-12"> | ||
<div class="col-md-6 col-md-offset-6 journal-right"> | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
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<div class="col-md-12 in-quote"> | <div class="col-md-12 in-quote"> | ||
<p>Presentation to high school students about Synthetic Biology for the Human Practices.</p> | <p>Presentation to high school students about Synthetic Biology for the Human Practices.</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/e/e8/Tec-chihuahua-notebook-july07.jpeg"> | |
| − | + | ||
| − | + | ||
</div> | </div> | ||
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<div class="col-md-12 in-quote"> | <div class="col-md-12 in-quote"> | ||
<p>F-NTC / Oocytes interaction and staining of the oocytes with DAPI.</p> | <p>F-NTC / Oocytes interaction and staining of the oocytes with DAPI.</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/7/7c/Tec-chihuahua-notebook-july10.jpeg"> | |
| − | + | ||
</div> | </div> | ||
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<p>We took a Biosafety Course for the Safety requirements.</p> | <p>We took a Biosafety Course for the Safety requirements.</p> | ||
<p>Wiki meeting with Sammy</p> | <p>Wiki meeting with Sammy</p> | ||
| − | |||
| − | |||
</div> | </div> | ||
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<div class="col-md-12 in-quote"> | <div class="col-md-12 in-quote"> | ||
<p>Team meeting.</p> | <p>Team meeting.</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/8/88/Tec-chihuahua-notebook-july15.jpeg"> | ||
</div> | </div> | ||
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<div class="col-md-12 in-quote"> | <div class="col-md-12 in-quote"> | ||
<p>Team meeting</p> | <p>Team meeting</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/5/58/Tec-chihuahua-notebook-july24.jpeg"> | |
</div> | </div> | ||
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<p>Research and documentary work.</p> | <p>Research and documentary work.</p> | ||
<p>Working on the wiki content.</p> | <p>Working on the wiki content.</p> | ||
| − | + | <img src="https://static.igem.org/mediawiki/2015/d/dd/Tec-chihuahua-notebook-july27.jpeg"> | |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | |||
<div class="col-md-12"> | <div class="col-md-12"> | ||
| − | + | <div class="col-md-6 journal-left"> | |
| − | + | <h3 class="quote-date"><b>Aug</b> / 03 / 2015</h3> | |
| − | + | <div class="col-md-12 in-quote"> | |
| − | + | <p>Wiki Content work</p> | |
| − | + | <p>Team meating</p> | |
| − | + | ||
| − | + | ||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| + | </div> | ||
| − | + | <div class="col-md-12"> | |
| − | + | <div class="col-md-6 col-md-offset-6 journal-right"> | |
| − | + | <h3 class="quote-date"><b>Aug</b> / 04 - 05 / 2015</h3> | |
| − | + | <div class="col-md-12 in-quote"> | |
| − | + | <p>Wiki meeting with Sammy</p> | |
| − | + | ||
| − | + | ||
</div> | </div> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| + | </div> | ||
| + | </div> | ||
| + | <div class="col-md-12"> | ||
| + | <div class="col-md-6 journal-left"> | ||
| + | <h3 class="quote-date"><b>Aug</b> / 06 / 2015</h3> | ||
| + | <div class="col-md-12 in-quote"> | ||
| + | <p>we grew <span class="bacteria">E. coli</span> TOP10 on LB brith and incubated it on shaker</p> | ||
| − | |||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| + | </div> | ||
| − | + | <div class="col-md-12"> | |
| − | + | <div class="col-md-6 col-md-offset-6 journal-right"> | |
| − | + | <h3 class="quote-date"><b>Aug</b> / 07 / 2015</h3> | |
| − | + | <div class="col-md-12 in-quote"> | |
| − | + | <p>Sterilizing solutions</p> | |
| − | + | <p>Aliquots of <span class="bacteria">E. coli</span> and glycerol were prepared</p> | |
| − | + | ||
| − | + | ||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| − | + | </div> | |
| − | + | <div class="col-md-12"> | |
| − | + | <div class="col-md-6 journal-left"> | |
| − | + | <h3 class="quote-date"><b>Aug</b> / 10 / 2015</h3> | |
| − | + | <div class="col-md-12 in-quote"> | |
| + | <p>Team meeting</p> | ||
| − | |||
| − | |||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| + | </div> | ||
| − | + | <div class="col-md-12"> | |
| − | + | <div class="col-md-6 col-md-offset-6 journal-right"> | |
| − | + | <h3 class="quote-date"><b>Aug</b> / 11 / 2015</h3> | |
| − | + | <div class="col-md-12 in-quote"> | |
| − | + | <p>Preparation of Solutions</p> | |
| − | + | <p>Preparation of LB Broth</p> | |
| − | + | <p>The optical density of TOP10 was measured</p> | |
| − | + | ||
| − | + | ||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| − | + | </div> | |
| − | + | <div class="col-md-12"> | |
| − | + | <div class="col-md-6 journal-left"> | |
| − | + | <h3 class="quote-date"><b>Aug</b> / 12 - 14 / 2015</h3> | |
| − | + | <div class="col-md-12 in-quote"> | |
| + | <p>Biobricks cloning started: We failed on cell transformation</p> | ||
| − | |||
| − | |||
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| − | + | </div> | |
| − | + | <div class="col-md-12"> | |
| − | + | <div class="col-md-6 col-md-offset-6 journal-right"> | |
| − | + | <h3 class="quote-date"><b>Sep</b> / 04 - 18 / 2015</h3> | |
| − | + | <div class="col-md-12 in-quote"> | |
| − | + | <p>Lab work with the biobricks</p> | |
| − | + | <p>Fast and Furiously Finishing the Wiki</p> | |
| − | + | <p>Last details of presentation and poster</p> | |
| − | + | <p>Making of promotional video.</p> | |
| − | + | <img src="https://static.igem.org/mediawiki/2015/3/35/Tec-chihuahua-notebook-sept02.jpeg"> | |
| − | + | ||
| − | </ | + | |
</div> | </div> | ||
| + | |||
</div> | </div> | ||
| + | </div> | ||
</br> | </br> | ||
<h1 class="arrow">Months of work finally come to end!</h1> | <h1 class="arrow">Months of work finally come to end!</h1> | ||
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| − | + | <!-- END OF THE JOURNAL!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! --> | |
| − | + | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 03:43, 19 September 2015
Notebook
Protocols
Oocytes
Parts
Plants
Journal
Feb / 26 / 2015
First meeting of the team. We talked about the proyect and started generating ideas.
Mar / 4 / 2015
Choice of the project.
Mar / 14 / 2015
We won the Entrepreneurship Contest of the Nucleum Congress in Monterrey.
Mar / 19 / 2015
Team Meeting.
Mar / 26 / 2015
Team promotional photo.
Abr / 2 / 2015
Team Meeting
Abr / 9 / 2015
We presented our ideas to experts of the Centro de Investigación en Materiales Avanzados (CIMAV), they seem interested on the Project and gave us their support.
Abr / 17 / 2015
We went to the local TV Show “Emma de la O” to talk about the biotechnology and our project.
Abr / 20 / 2015
Synthesis of the NP-CNT. We began the synthesis of the nanotubes at CIMAV.
May / 12 - 15 / 2015
Purification of the NP-CNT
May / 20 / 2015
Filtration of the P-CNT
May / 21 / 2015
Preparation of Luria Bertani Broth for E. coli
May / 22 / 2015
We finished the filtration of the nanotubes
Plasmid extraction of E. coli
May / 25 / 2015
We measured the purity of plasmid using the NanoDrop 2000
May / 27 / 2015
Team meeting to discuss about the wiki
May / 28 / 2015
Inventory of team´s material and lab cleanup.
Jun / 1 / 2015
Preparation of Luria Bertani agar.
Team meeting for fund collection.
Sterilizing of work solutions.
We began to dry the P-CNT
Jun / 3 / 2015
We finished drying the nanotubes.
Preparation of PBS
Jun / 8 / 2015
We began the work with the bovine oocytes at the Facultad de Zootecnia (School of Animal Husbandry) of the Universidad Autónoma de Chihuahua (UACH).
Jun / 9 / 2015
Arriving of the Florescein Diacetate.
Oocytes extraction and selection.
The maduration of the viables oocytes began.
Jun / 10 / 2015
We began the CNT-Fluorescein functionalization.
Cleaning and picking of the oocytes.
Jun / 11 / 2015
We finished the CNT functionalization with fluorescein.
Jun / 15 / 2015
Pins Ceremony
Jun / 16 / 2015
F-NTC / Oocytes interaction. We failed
Jun / 18 / 2015
Purification of the Second batch of CNT.
Jun / 22 / 2015
Preparation of the stocks and the media for the handling and maduration of oocytes.
Jun / 24 / 2015
Oocytes extraction and selection.
The maduration of the viables oocytes began.
Jun/ 25 / 2015
Cleaning and picking of the oocytes.
F-NTC / Oocytes interaction.
Filtration of the second group of nanotubes
Jun / 26 / 2015
“About our lab” and “About our proyect” Questionnaires.
Jun / 29 / 2015
Team meeting.
Observation of functionalized oocytes with confocal microscope.
Jun / 30 / 2015
Filtration of the CNT.
Jul / 1 / 2015
We finished the filtration of the second batch of CNT.
Jul / 2 / 2015
Drying of the P-CNT.
Oocytes extraction and selection.
Preparation of work solutions.
Jul / 3 / 2015
Preparation of work solutions.
Jul / 4 / 2015
F-NTC / Oocytes interaction and staining of the oocytes with DAPI.
Jul / 6 /2015
Team meeting
Jul / 7 / 2015
Presentation to high school students about Synthetic Biology for the Human Practices.
Jul / 8 / 2015
Oocytes extraction and selection for maduration
We fabricated glass pipettes
Wiki meeting with Sammy.
Jul / 9 / 2015
Cleaning and picking of the oocytes.
Jul / 10 / 2015
F-NTC / Oocytes interaction and staining of the oocytes with DAPI.
Jul / 13 / 2015
We took a Biosafety Course for the Safety requirements.
Wiki meeting with Sammy
Jul / 15 / 2015
Team meeting.
Jul / 16 / 2015
Wiki meeting with Sammy.
Jul / 17 / 2015
Preparation of work solutions.
Jul / 20 / 2015
Meeting with Dr. Quintín Rascon to planning the work on N. tabacum.
Jul / 21 / 2015
We started Functionalization with Fluorescein of the second batch of P-CNT.
Oocytes extraction and selection for maduration.
Jul / 22 / 2015
We finished the CNT-functionalization.
Jul / 23 / 2015
Preparation of solutions.
Interaction of the oocytes with the F-CNT (both of the batches), previously fixed with paraformaldehyde.
Jul / 24 / 2015
Team meeting
Jul / 27 - 31 / 2015
Research and documentary work.
Working on the wiki content.
Aug / 03 / 2015
Wiki Content work
Team meating
Aug / 04 - 05 / 2015
Wiki meeting with Sammy
Aug / 06 / 2015
we grew E. coli TOP10 on LB brith and incubated it on shaker
Aug / 07 / 2015
Sterilizing solutions
Aliquots of E. coli and glycerol were prepared
Aug / 10 / 2015
Team meeting
Aug / 11 / 2015
Preparation of Solutions
Preparation of LB Broth
The optical density of TOP10 was measured
Aug / 12 - 14 / 2015
Biobricks cloning started: We failed on cell transformation
Sep / 04 - 18 / 2015
Lab work with the biobricks
Fast and Furiously Finishing the Wiki
Last details of presentation and poster
Making of promotional video.
