Difference between revisions of "Team:UCL/Effectors"

 
(44 intermediate revisions by 3 users not shown)
Line 57: Line 57:
  
 
</script>
 
</script>
 +
<script type="text/javascript" src="https://2015.igem.org/Team:UCL/Smoothscroll?action=raw&amp;ctype=text/javascript"></script>
  
 +
  <script>
 +
smoothScroll.init();
 +
</script>
 +
 
 +
 
 
<style>
 
<style>
  
Line 63: Line 69:
 
.mainmenu {background-color: #fff !important;}
 
.mainmenu {background-color: #fff !important;}
 
.menu-items a {color: #493C54;}
 
.menu-items a {color: #493C54;}
.buttonblack {background-color: #22343C; border-color: #22343C; }
+
.buttonblack {background-color: #336666; border-color: #336666; }
 
a.buttonblack { color: #fff !important; font-size: 12px;}
 
a.buttonblack { color: #fff !important; font-size: 12px;}
.buttonblack:hover {border-color: #22343C; color: #22343C !important;}
+
.buttonblack:hover {border-color: #336666; color: #336666 !important;}
  
  
Line 164: Line 170:
 
.content {color: #d67166;}
 
.content {color: #d67166;}
  
.bigtitle {width: 42%; top: 0; margin: 0 auto; font-size: 16px; background-image: url('https://static.igem.org/mediawiki/2015/4/43/UCL_headercircle.png'); text-align: center; line-height: 2;
 
    background-position: top center;  background-repeat: no-repeat; background-size: 600px; padding: 9%; position: relative; margin-bottom: 100px; color: #22343C;}
 
  
 
.bigtitle a {color: #22343C; text-decoration: none;}
 
.bigtitle a {color: #22343C; text-decoration: none;}
  
.title2 {font-family: Allura; font-size: 450%;}
+
.title2 {font-size: 450%; line-height: 1.5; }
  
  
.titlecircle {width: 85%; margin: 0 auto; padding-top: 7%;}
+
.titlecircle {width: 85%; margin: 0 auto; padding-top: 6%;}
  
 
.arrow {font-size: 50px;}
 
.arrow {font-size: 50px;}
  
  
 
+
.memberwrapper p {
 
+
text-align:justify;
#text h2 {
+
padding: 0 8px !important;
font-size: 30px !important;
+
font-size: 15px;
 +
line-height: 1.6;
 +
letter-spacing: 1.2px;
 +
color: #0a0111;
 +
margin: 0 !important;
  
 
}
 
}
 +
  
  
Line 191: Line 200:
  
 
#text {margin-top: 50px; z-index: 9999;}
 
#text {margin-top: 50px; z-index: 9999;}
#text h2 {font-size: 20px; text-decoration: none; border-bottom: 0px; display: block; background-color: #c97062; color: #fff; font-weight: bold; border-radius: 4px; padding: 10px; opacity: 0.5; }
+
#text h2 {font-size: 25px; text-decoration: none; border-bottom: 0px; display: block; background-color: #c97062; color: #fff; font-weight: bold; border-radius: 4px; padding: 10px; opacity: 0.5; }
  
 
.blockk:hover h2 {opacity: 0.9 !important; transition-property: opacity;
 
.blockk:hover h2 {opacity: 0.9 !important; transition-property: opacity;
Line 396: Line 405:
  
 
<div class="bigtitle">
 
<div class="bigtitle">
<div class="titlecircle"><span class="title2">Psychobiotics</span> <p style="margin-top: 0px; line-height: 1.7;margin-left:10%;margin-right:9%;height:50%;"> are an emerging class of probiotics that provide benefit to psychological health of patients by secreting compounds which affect nervous system. We have applied synthetic biology to incorporate the natural neuroregulatory properties of gut bacteria into synthetic genetic devices. <br><span class="arrow"> <a data-scroll data-options='{ "easing": "linear" }' href="#submenu" >&#x25BC; </a></span>
+
<div class="titlecircle"><span class="title2">Psychobiotics</span><hr style="height:1px; border:none; color:#22343; background-color:#000;"><p style="margin-top: 0px; line-height: 1.7; height:50%;"> are an emerging class of probiotics that provide benefit to psychological health of patients by secreting compounds which affect the nervous system. We have applied synthetic biology to incorporate the natural neuroregulatory properties of gut bacteria into synthetic genetic devices. <br><span class="arrow"> <a data-scroll data-options='{ "easing": "linear" }' href="#submenu" >&#x25BC; </a></span>
  
 
</p>
 
</p>
Line 474: Line 483:
 
  <a href="http://www.ncbi.nlm.nih.gov/pubmed/25860609"><b>[Link]</b></a></span><b>[9]</b></span>. We have created a synthetic device that produces <b>functional human tryptophan hydroxylase</b> to restore healthy serotonin levels in affected patients.
 
  <a href="http://www.ncbi.nlm.nih.gov/pubmed/25860609"><b>[Link]</b></a></span><b>[9]</b></span>. We have created a synthetic device that produces <b>functional human tryptophan hydroxylase</b> to restore healthy serotonin levels in affected patients.
 
</div>  <br>
 
</div>  <br>
 
+
  </h4>
 
<div class="constructsdiv">
 
<div class="constructsdiv">
 
<p class="constructs">
 
<p class="constructs">
Line 498: Line 507:
  
 
    
 
    
    
+
   <h4>
 
    
 
    
 
   <span style="font-size: 20px;"><b>Characterization</b></span><br><br>
 
   <span style="font-size: 20px;"><b>Characterization</b></span><br><br>
 +
 +
  <b> Expression of IPTG-inducible TPH1 (BBa_K1598002) </b><br><br>
 +
 +
Instability and insolubility of human TPH1 expressed in <i>E. coli</i> has been previously reported  <span class="content"><span class="tooltip">Kowlessur, D. and Kaufman, S. (1999). Cloning and expression of recombinant human pineal tryptophan hydroxylase in Escherichia coli: purification and characterization of the cloned enzyme. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1434(2), pp.317-330.
 +
    <a href="http://www.sciencedirect.com/science/article/pii/S0167483899001843"><b>[Link]</b></a>s</span>
 +
    <b>[23]</b></span>. In order to overcome the stability issues of our TPH enzyme, the induced bacteria were cultured overnight at room temperature, as suggested by previous research <span class="content"><span class="tooltip">Kowlessur, D. and Kaufman, S. (1999). Cloning and expression of recombinant human pineal tryptophan hydroxylase in Escherichia coli: purification and characterization of the cloned enzyme. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1434(2), pp.317-330.
 +
    <a href="http://www.sciencedirect.com/science/article/pii/S0167483899001843"><b>[Link]</b></a>s</span>
 +
    <b>[24]</b></span>. On the following morning cells were lysed, and protein expression was confirmed and quantified using Bradford assay with Bovine Serum Albumin as a standard. <br><br>
 +
 +
 +
<div style="display: block;">
 +
<div style="margin: 0 auto; font-size: 13px; line-height: 1.2; width: 48%; float: left;">
 +
<img src="https://static.igem.org/mediawiki/2015/6/63/UCL_Bassay.png" style="width: 100%;"> <br><b>Standard curve for Bradford Assay. The absorbance of 10-fold diluted samples of induced lysate and uninduced control is indicated on the graph.</b>
 +
<div style="height: 150px;"></div></div>
 +
<div style="margin: 0 auto; font-size: 13px; line-height: 1.2; width: 48%; float: right;">
 +
<img src="https://static.igem.org/mediawiki/2015/8/8b/UCL_Graph4.png" style="width: 100%;"><br><b>Protein concentration in induced lysate and uninduced control quantified using Bradford Assay</b></div>
 +
</div>
 +
<br><br><Br>
 +
 +
<b> Enzyme activity of IPTG-inducible TPH1 (BBa_K1598002) </b><br><br>
 +
 +
In order to characterize our biobrick, we have adapted the fluorometric assay developed by Moran and Fitzpatrick, which exploit difference in spectral properties of tryptophan and 5-HTP <span class="content"><span class="tooltip">Moran, G. and Fitzpatrick, P. (1999). A Continuous Fluorescence Assay for Tryptophan Hydroxylase. Analytical Biochemistry, 266(1), pp.148-152.
 +
    <a href="http://www.ncbi.nlm.nih.gov/pubmed/9887224"><b>[Link]</b></a>s</span>
 +
    <b>[25]</b></span>. According to the authors, tryptophan exhibits negligible fluorescence above 300 nm, and using excitation wavelength of 300 nm results in 5-hydroxytryptophan being almost exclusively excited. The emission is measured at 330 nm. As the assay was reported to be suitable for unpurified samples, we have performed fluorescence measurements using crude cell lysates. The detailed characterization protocol is available here: <a href="https://static.igem.org/mediawiki/2015/c/cb/UCL_BBa_K1598002_Assay.pdf">pdf</a> or in <a href="https://2015.igem.org/Team:UCL/Protocols">Protocols</a> section. <br><br>
 +
 +
 +
In order to demonstrate the TPH1 activity, samples containing range of crude lysate concentrations were added to the appropriate assay buffer and the reactions were initiated by adding 120 uM tryptophan solution. For blank measurements, uninduced control was used instead of induced sample.  <br><br>
 +
 +
 +
 +
<div style=" margin: 0 auto; font-size: 13px; line-height: 1.2; width: 60%;">
 +
<img src="https://static.igem.org/mediawiki/2015/0/05/UCLg7.png" style="width: 100%;">
 +
<br><b>Increase in fluorescence over time indicates 5-HTP production. The reported fluorescence values are shown as means of 8 replicates +/- SD. All measurements are expressed as ratio of fluorescence of the sample to maximum fluorescence intensity observed </b>.</div><br><Br>
  
 
    
 
    
 
+
 
 +
 
 
    
 
    
 
   <span style="font-size: 20px;"><b>Anti-TNA Silencing RNAs</b></span><br><br>
 
   <span style="font-size: 20px;"><b>Anti-TNA Silencing RNAs</b></span><br><br>
Line 513: Line 556:
  
 
    
 
    
    
+
   </h4>
 
<br>
 
<br>
  
 
    
 
    
 
   <h2><span id="Acetylcholine" style="padding-top: 150px;">Amazing Artificial Acetylcholine Apparatus</span> </h2>
 
   <h2><span id="Acetylcholine" style="padding-top: 150px;">Amazing Artificial Acetylcholine Apparatus</span> </h2>
 
+
<h4>
 
<span style="font-size: 20px;"><b>Description</b></span><br><Br>
 
<span style="font-size: 20px;"><b>Description</b></span><br><Br>
  
Line 543: Line 586:
 
<Br><Br>
 
<Br><Br>
  
 
+
  </h4>
 
<div class="constructsdiv">
 
<div class="constructsdiv">
 
<p class="constructs">
 
<p class="constructs">
Line 559: Line 602:
 
     <h2><span id="gaba" style="padding-top: 150px;">GABA Generating Gut Germs</span> </h2>
 
     <h2><span id="gaba" style="padding-top: 150px;">GABA Generating Gut Germs</span> </h2>
  
    
+
   <h4>
 
   <span style="font-size: 20px;"><b>Description</b></span><br><br>
 
   <span style="font-size: 20px;"><b>Description</b></span><br><br>
  
Line 582: Line 625:
 
<Br><br>
 
<Br><br>
  
 
+
  </h4>
 
<div class="constructsdiv">
 
<div class="constructsdiv">
 
<p class="constructs">
 
<p class="constructs">
Line 600: Line 643:
 
<br/> <br/>
 
<br/> <br/>
 
    
 
    
       <h2><span id="pregnenenen" style="padding-top: 150px;">Targeting Side Effects with Pregnenolone</span> </h2>
+
       <h2><span id="Pregnenenen" style="padding-top: 150px;">People Power Personal Pregnenolone</span> </h2>
  
    
+
   <h4>
 
<span style="font-size: 20px;"><b>Description</b></span><br><br>
 
<span style="font-size: 20px;"><b>Description</b></span><br><br>
  
<p>Also called Cytochrome P450scc, Cholesterol Side Chain Cleavage Enzyme (P450scc) is a mitochondrial enzyme that converts cholesterol into pregnenelone, which is a neurosteroid as well as the precursor to a variety of steroid hormones and neurotransmitters. The gene that encodes it is CYP11A1.
+
<p>Also called Cytochrome P450scc, Cholesterol Side Chain Cleavage Enzyme (P450scc) is a mitochondrial enzyme that converts cholesterol into pregnenalone, which is a neurosteroid as well as the precursor to a variety of steroid hormones and neurotransmitters. The gene that encodes it is CYP11A1.
  
 
<p>This is the reaction it catalyses: </p>
 
<p>This is the reaction it catalyses: </p>
Line 611: Line 654:
  
 
<p>Furthermore, the catalysis involves 3 monooxygenase reactions, which requires two electron transport proteins: ferrodoxin (FDX1) and ferredoxin reductase (FDXR).</p>
 
<p>Furthermore, the catalysis involves 3 monooxygenase reactions, which requires two electron transport proteins: ferrodoxin (FDX1) and ferredoxin reductase (FDXR).</p>
 
<br>
 
<img src="https://static.igem.org/mediawiki/2015/1/18/Pregnenelone_comic.PNG">
 
</br>
 
  
 
<p>Our biobrick consists of the iGEM prefix followed by the J23101 constitutive promoter, RBS, FDXR gene, RBS, FDX1 GENE, RBS, CYP11A1 gene, rrnb double terminator, and the iGEM suffix. This will be ligated onto a PSB1C3 backbone.
 
<p>Our biobrick consists of the iGEM prefix followed by the J23101 constitutive promoter, RBS, FDXR gene, RBS, FDX1 GENE, RBS, CYP11A1 gene, rrnb double terminator, and the iGEM suffix. This will be ligated onto a PSB1C3 backbone.
Line 730: Line 769:
  
 
ALL NICE BUT NOT RELEVANT TO OUR PROJECT-->
 
ALL NICE BUT NOT RELEVANT TO OUR PROJECT-->
 +
 +
<h3>Results</h3>
 +
 +
<p>We ran an HPLC assay for the pregnenelone biobrick to characterise its function.
 +
Pregenolone was seen as a clear peak at around 11mins.
 +
 +
A standard curve was plotted based on the peaks. The standard curve was then used to calculate the concentration of pregnenolone produced by each sample:
 +
 +
<ul>
 +
<li>Sample with no cholesterol- 0mg/ml</li>
 +
<li>Sample with cholesterol in 2-hydroxypropyl beta cyclodextrin- 0.0007mg/ml</li>
 +
<li>Sample with cholesterol in DMSO- 0.007mg/ml</li>
 +
</ul>
 +
 +
<b>Therefore, we got a maximum pregnenolone production of 0.007 mg/ml within 4 hours of substrate addition!</b>
 +
</p>
 
</p>
 
</p>
  
Line 737: Line 792:
 
</div>
 
</div>
  
<h2><span id="modelling"  style="padding-top: 100px;"> Modelling</span></h2>
 
<h4></h4>
 
 
<h2><span id="characterization"  style="padding-top: 100px;"> Characterization</span></h2>
 
<h4></h4>
 
  
  </div>
 
  
 
</div>
 
</div>

Latest revision as of 03:59, 19 September 2015

'

Psychobiotics

are an emerging class of probiotics that provide benefit to psychological health of patients by secreting compounds which affect the nervous system. We have applied synthetic biology to incorporate the natural neuroregulatory properties of gut bacteria into synthetic genetic devices.

Background

Gut-brain communication involves biochemical signalling between intestinal microflora and host's central nervous system. These interactions are bidirectional: gut microbiome is influenced by our mental state and, at the same time, is capable of affect our mental well-being. The term psychobiotics describes an emerging class of probiotics that positively affect patient's mental health by producing neuroactive compounds, including neurotransmitters. The molecules associated with mammalian neurotransmission that have been isolated from gut bacteria include gamma-butyric acid (GABA), serotonin, dopamine, and acetylcholine Lyte, M. and Cryan, J. Microbial Endocrinology: The Microbiota-Gut-Brain Axis in Health and Disease Advances in Experimental Medicine and Biology Volume 817, 2014, pp 221-239 [Link][1]. When secreted into intestinal lumen, these molecules can modulate the neural signalling within the enteric nervous system which communicates with brain via the vagus nerve, therefore influencing brain’s biochemistry and host’s behaviour.

“It is well recognized that some bacteria within the human gut have the capacity to produce many neurotransmitters and neuromodulators. For example, Lactoba cillus spp. and Bifidobacterium spp. have been reported to produce GABA; Escherichia spp., and Bacillus spp. have been reported to produce norepinephrine; Streptococcus spp., Escherichia spp. and Enterococcus spp. have been reported to produce serotonin; Bacillus spp. have been reported to produce dopamine, and Lactobacillus spp. have been reported to produce acetylcholine and histamine. [...] Other bacterially-produced metabolites with proven neuroactive func- tions include short chain fatty acids (SCFAs) and long chain fatty acids such as conjugated linoleic acid (CLA). “
Microbial Endocrinology: The Microbiota-Gut-Brain Axis in Health and Disease Advances in Experimental Medicine and Biology Volume 817, 2014, pp 221-239



Probiotics are live microorganisms that, when ingested in adequate amounts, exert a health benefit on the host Report on Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria, 2001. [Link][2]. The studies on mice indicate that several natural probiotic strains exhibit psychobiotic properties: treatment with Lactobacillus rhamnosus has been shown to regulate emotional behaviour and influence the mRNA expression of GABA receptor in host’s brain Bravo, Javier A, Forsythe, Paul, Chew, Marianne V, Escaravage, Emily; Savignac, Hélène M et al. Ingestion of Lactobacillus strain regulates emotional behavior and central GABA receptor expression in a mouse via the vagus nerve. Proceedings of the National Academy of Sciences of the United States of America vol. 108 (38), 2011, p. 16050-5 [Link][3], while another study has shown that administration of Bifidobacterium infantis results in increased concentration of tryptophan, a serotonergic precursor, in blood plasma and shows antidepressant properties as determined by forced swim test Desbonnet, L., Garrett, L., Clarke, G., Bienenstock, J., & Dinan, T. G. The probiotic Bifidobacteria infantis: An assessment of potential antidepressant properties in the rat. Journal of Psychiatric Research, 43(2), 2008, p. 164–174 [Link] [4]. Based on the current understanding of the role of commensal microbiota in regulation of host brain biochemistry and behaviour, we hypothesize that by engineering bacteria for precisely controlled neurochemical production we can develop probiotic strains that could potentially act as new strategies for mental health treatment.

Synthetic Super Serotonin System

The genes for our constructs were chosen based on the analysis of metabolic pathways involved in synthesis of neurotransmitter compounds, including tryptophan pathway, glutamate pathway, and acetylcholine pathway. The design of the constructs is explained below. The experimental protocols can be accessed here.

Description

Serotonin is an essential metabolite, key neurotransmitter in mood regulation and a target of vast majority of antidepressant drugs. The majority of serotonin in the body is produced by enterochromaffin cells located in the gut Manocha, M. and Khan, W. Serotonin and GI Disorders: An Update on Clinical and Experimental Studies. Clinical and Translational Gastroenterology, 2012, 3(4), p.e13. [Link][6]. Metabolomics study has revealed that serotonin levels in blood plasma are 2.8 fold higher in conventional as opposed to germ-free mice Wikoff, W., Anfora, A., Liu, J., Schultz, P., Lesley, S., Peters, E. and Siuzdak, G. Metabolomics analysis reveals large effects of gut microflora on mammalian blood metabolites. Proceedings of the National Academy of Sciences, 2009, 106(10), pp.3698-3703. [Link][7], suggesting that the interactions between host and gut microflora play crucial role in regulating the production of serotonin and functioning of serotonergic system.
Our system addresses serotonin deficiency and dysregulation of gut-brain communication in patients with mental health disorders.

The rate-limiting step of synthesis of serotonin is catalyzed by tryptophan hydroxylase, TPH, which converts tryptophan, an essential amino acid, into 5-hydroxytryptophan (5-HTP) O’Mahony, S., Clarke, G., Borre, Y., Dinan, T. and Cryan, J. Serotonin, tryptophan metabolism and the brain-gut-microbiome axis. Behavioural Brain Research, 2015, 277, pp.32-48. [Link][8]. It was shown that microbial colonization of the gut is essential for maintaining normal levels of tryptophan hydroxylase and serotonin in the blood Yano, J., Yu, K., Donaldson, G., Shastri, G., Ann, P., Ma, L., Nagler, C., Ismagilov, R., Mazmanian, S. and Hsiao, E. Indigenous Bacteria from the Gut Microbiota Regulate Host Serotonin Biosynthesis. Cell, 2015, 161(2), pp.264-276. [Link][9]. We have created a synthetic device that produces functional human tryptophan hydroxylase to restore healthy serotonin levels in affected patients.

Our Constructs

BBa_K1598001 - the basic TPH1 part. The consensus sequence of human TPH1 was obtained from UniProt (accession no: P17752). The sequence was codon-optimized for E.coli and adapted to BioBrick format. The construct was synthesized by IDT in a form of gBlock gene fragment and cloned into PSB1C3 using traditional digestion-ligation cloning.


BBa_K1598002 - the IPTG-inducible construct containing the Lac expression cassete (BBa_413103) with the TPH1 gene (BBa_K1598001) - Lac expression cassette was obtained from the iGEM distribution. The parts were assembled using the gel extraction method.





Characterization

Expression of IPTG-inducible TPH1 (BBa_K1598002)

Instability and insolubility of human TPH1 expressed in E. coli has been previously reported Kowlessur, D. and Kaufman, S. (1999). Cloning and expression of recombinant human pineal tryptophan hydroxylase in Escherichia coli: purification and characterization of the cloned enzyme. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1434(2), pp.317-330. [Link]s [23]. In order to overcome the stability issues of our TPH enzyme, the induced bacteria were cultured overnight at room temperature, as suggested by previous research Kowlessur, D. and Kaufman, S. (1999). Cloning and expression of recombinant human pineal tryptophan hydroxylase in Escherichia coli: purification and characterization of the cloned enzyme. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1434(2), pp.317-330. [Link]s [24]. On the following morning cells were lysed, and protein expression was confirmed and quantified using Bradford assay with Bovine Serum Albumin as a standard.


Standard curve for Bradford Assay. The absorbance of 10-fold diluted samples of induced lysate and uninduced control is indicated on the graph.

Protein concentration in induced lysate and uninduced control quantified using Bradford Assay



Enzyme activity of IPTG-inducible TPH1 (BBa_K1598002)

In order to characterize our biobrick, we have adapted the fluorometric assay developed by Moran and Fitzpatrick, which exploit difference in spectral properties of tryptophan and 5-HTP Moran, G. and Fitzpatrick, P. (1999). A Continuous Fluorescence Assay for Tryptophan Hydroxylase. Analytical Biochemistry, 266(1), pp.148-152. [Link]s [25]. According to the authors, tryptophan exhibits negligible fluorescence above 300 nm, and using excitation wavelength of 300 nm results in 5-hydroxytryptophan being almost exclusively excited. The emission is measured at 330 nm. As the assay was reported to be suitable for unpurified samples, we have performed fluorescence measurements using crude cell lysates. The detailed characterization protocol is available here: pdf or in Protocols section.

In order to demonstrate the TPH1 activity, samples containing range of crude lysate concentrations were added to the appropriate assay buffer and the reactions were initiated by adding 120 uM tryptophan solution. For blank measurements, uninduced control was used instead of induced sample.


Increase in fluorescence over time indicates 5-HTP production. The reported fluorescence values are shown as means of 8 replicates +/- SD. All measurements are expressed as ratio of fluorescence of the sample to maximum fluorescence intensity observed .


Anti-TNA Silencing RNAs

The functioning of our serotonin-enhancing system relies strongly on the tryptophan availability in the gut. Several species present in gut microflora, including Escherichia spp. express tryptophanase, which performs degradation of tryptophan into indole, ammonia and pyruvate Chimerel, C., Emery, E., Summers, D., Keyser, U., Gribble, F. and Reimann, F. Bacterial Metabolite Indole Modulates Incretin Secretion from Intestinal Enteroendocrine L Cells. Cell Reports, 2014, 9(4), pp.1202-1208.[Link][10]. We propose to use antisense RNA complementary to tryptophanase-encoding gene as a strategy for downregulating the breakdown of tryptophan in the gut environment and, thus, optimization of 5-HTP synthesis.
In E. coli tryptophanase is encoded by tnaA gene. We have designed two synthetic regulatory small RNAs and investigated their applicability and sensitivity in silencing gene expression.


Amazing Artificial Acetylcholine Apparatus

Description

Acetylcholine is a versatile neurotransmitter that mediates signalling across central and peripheral nervous system. Acetylcholine activity is also essential for regulating the activity of vagus nerve, which connects brain to digestive system and Lleó, A., Greenberg, S. and Growdon, J. Current Pharmacotherapy for Alzheimer's Disease. Annual Review of Medicine, 2006, 57(1), pp.513-533.[Link][12], and preventing the excessive production of inflammatory cytokines [13]. These inflammatory cytokines are elevated in patients with major depression Dowlati, Y., Herrmann, N., Swardfager, W., Liu, H., Sham, L., Reim, E. and Lanctôt, K. (2010). A Meta-Analysis of Cytokines in Major Depression. Biological Psychiatry, 67(5), pp.446-457. [Link][14] and have been shown to induce depressive-like behaviour in mice Larson, S. and Dunn, A. Behavioral Effects of Cytokines. Brain, Behavior, and Immunity, 2001, 15(4), pp.371-387.[Link][15] and humans Capuron, L., Ravaud, A., Neveu, P., Miller, A., Maes, M. and Dantzer, R. Association between decreased serum tryptophan concentrations and depressive symptoms in cancer patients undergoing cytokine therapy. Molecular Psychiatry, 2002, 7(5), pp.468-473.[Link][16].

The synthesis of acetylcholine is catalysed by choline acetyltransferase, CHAT. In humans, CHAT expression requires microbiota-dependent signalling and does not begin until microbial gut colonization after birth. Reardon, C., Duncan, G., Brustle, A., Brenner, D., Tusche, M., Olofsson, P., Rosas-Ballina, M., Tracey, K. and Mak, T. Lymphocyte-derived ACh regulates local innate but not adaptive immunity. Proceedings of the National Academy of Sciences, 2013, 110(4), pp.1410-1415.[Link][17]. Hence, imbalances in gut microflora can lead to disrupted acetylcholine activity and associated depression-related symptoms.

We created a genetic device for inducible expression of human CHAT and explored the acetylcholine-producing probiotics as a solution for restoring the acetylcholine levels in patients with depression and other psychiatric diseases.

Our Constructs

BBa_K1598003 - basic CHAT construct. The consensus sequence of human CHAT was obtained from UniProt (accession no: P28329). The sequence was codon-optimized for E.coli and 6xHis tag was added on C-terminal. BBa_B0034 RBS and BBa_B0015 terminator were added upstream and downstream of the gene, respectively. The construct was synthesized by IDT in a form of 2 gBlock gene fragments (1013bp and 1182bp with 30bp overlap) and cloned into PSB1C3 using Gibson assembly.




GABA Generating Gut Germs

Description

GABA is the main inhibitory neurotransmitter in the brain and decreased GABA levels have been linked to depression and anxiety Kalueff, A. and Nutt, D. (2007). Role of GABA in anxiety and depression. Depression and Anxiety, 24(7), pp.495-517.[Link][18]. Several species of gut bacteria have been shown to produce GABA Cryan, J. and Dinan, T. (2012). Mind-altering microorganisms: the impact of the gut microbiota on brain and behaviour. Nature Reviews Neuroscience, 13(10), pp.701-712.[Link][19] and metabolomics analysis has shown that the gut luminal GABA levels in ex-germfree mice are considerably higher than those observed in germfree mice Matsumoto, M., Kibe, R., Ooga, T., Aiba, Y., Kurihara, S., Sawaki, E., Koga, Y. and Benno, Y. (2012). Impact of Intestinal Microbiota on Intestinal Luminal Metabolome. Sci. Rep., 2.[Link][20] . The role of microbiota-derived GABA in gut-brain signalling can be supported by the presence of GABA receptors in gut epithelial cells Li, Y., Xiang, Y., Lu, W., Liu, C. and Li, J. (2012). A novel role of intestine epithelial GABAergic signaling in regulating intestinal fluid secretion. AJP: Gastrointestinal and Liver Physiology, 303(4), pp.G453-G460. [Link][21].

Our synthetic probiotics produce GABA through reaction catalyzed by glutamate decarboxylase (GAD).

The positive effects of GABA-producing species in the gut on the emotional behaviour of the host have been previously documented. In one study, administration of milk enriched in GABA-producing Lactobacillus brevis FPA 3709 had antidepressant effect on mice Ko, C., Lin, H. and Tsai, G. (2013). Gamma-aminobutyric acid production in black soybean milk by Lactobacillus brevis FPA 3709 and the antidepressant effect of the fermented product on a forced swimming rat model. Process Biochemistry, 48(4), pp.559-568. [Link] [22] . Another study has shown that ingestion of Lactobacillus rhamnosus reduces the stress-induced and depression-related behaviour Bravo, J., Forsythe, P., Chew, M., Escaravage, E., Savignac, H., Dinan, T., Bienenstock, J. and Cryan, J. (2011). Ingestion of Lactobacillus strain regulates emotional behavior and central GABA receptor expression in a mouse via the vagus nerve. Proceedings of the National Academy of Sciences, 108(38), pp.16050-16055.[Link][23] . Therefore, we suggest to investigate the therapeutic potential of administration of our GAD-overexpressing probiotics to patients with decreased GABA levels.

Our Constructs

BBa_K1598004 - the basic GAD part. The consensus sequence of human TPH1 was obtained from UniProt (accession no: Q99259). The sequence was codon-optimized for E.coli and 6xHis tag was added on C-terminal. BBa_B0034 RBS and BBa_B0015 terminator were added upstream and downstream of the gene, respectively. The construct was synthesized by IDT in a form of gBlock gene fragment and cloned into PSB1C3 using traditional digestion-ligation cloning.



People Power Personal Pregnenolone

Description

Also called Cytochrome P450scc, Cholesterol Side Chain Cleavage Enzyme (P450scc) is a mitochondrial enzyme that converts cholesterol into pregnenalone, which is a neurosteroid as well as the precursor to a variety of steroid hormones and neurotransmitters. The gene that encodes it is CYP11A1.

This is the reaction it catalyses:

cholesterol + 3 NADPH + 3 H+ + 3 O2 ⇄ pregnenolone + 4-methylpentanal + 3 NADP+ + 3 H2O

Furthermore, the catalysis involves 3 monooxygenase reactions, which requires two electron transport proteins: ferrodoxin (FDX1) and ferredoxin reductase (FDXR).

Our biobrick consists of the iGEM prefix followed by the J23101 constitutive promoter, RBS, FDXR gene, RBS, FDX1 GENE, RBS, CYP11A1 gene, rrnb double terminator, and the iGEM suffix. This will be ligated onto a PSB1C3 backbone.



DNA Assembly

Cloning Flowsheet

Our biobrick (excluding the vector) has been split into 5 parts for synthesis. Once they arrive, they will be assembled together using Golden Gate Assembly by the experts at Synthace, one of our sponsors. Sap1I will be the enzyme used according to the standard Golden Gate assembly protocol.

  • Day 1: PCR Amplification and Purification of parts, followed by Digestion.
  • Day 2: Gel Purification, Golden Gate Assembly, and Transformation using DH5 alpha cells.
  • Day 3: Picking of colonies and Inoculation
  • Day 4: Minipreps, Digestion using ECOR1 and Pst1, Ligation with PSB1C3, and Transformation
  • Day 5: Picking of Colonies, and Inoculation
  • Day 6: Creation of Glycerol Stocks and Characterization using HPLC.

The protocols for all the steps describe above can be found on the protocols section of this wiki.

Golden Gate Assembly Protocol:

For assembling parts that are in Golden Gate standard, we used the following protocol:

Components Amount (μl)
Sap1(15 U) 0,75
T4 Ligase (400 U) 1
DTT (10 mM) 1
ATP (10 mM) 1
G-Buffer (10x, Fermentas) 1
parts 40 fmoles each
ddH2O Fill up to 10
Total Volume 10

Thermocycler programme:

  1. 37°C, 5 min
  2. 20°C, 5 min
  3. repeat (1. and 2.) 50 times
  4. 50°C, 10 min
  5. 80°C, 10 min

Characterization Assay (HPLC): The steps were followed according to the protocol given in this paper.

Relief of Side-effects from Depression/Anxiety drugs:

Participant A, a long term depression patient we interviewed, suggested that our novel probiotics will be more accepted if they had reduced the side-effects when compared to conventional drugs. She also mentioned how drugs gradually became ineffective overtime, and the dosage needs to be constantly increased, which is a major financial burden. Well, the administration of Pregnenolone and its derivates prevents the development of tolerance, and augments recovery from benzodiazepine withdrawal anxiety and hyperactivity in mice. Benzodiazepines, such as Valium, are one of the most popular antidepressant/anti-anxiety drugs in the world! Basically, this removes the need to constantly increase the dosage, and gets rid of many side effects and withdrawal symptoms, associated with treatments that act on the GABA receptor. Hence, combining it, pregnenolone, with our biobricks creates a complete system, which not only treat mental illness but also reduce side effects of treatment! Hence we have expanded on our silver medal Human Practices activity by demonstrating how we have integrated the investigated issues into the design and/or execution of your project. [reference 1]

·

Depression/Anxiety:

Neurosteroids derived from Pregnenolone modulate several neurotransmitter systems such as gamma-aminobutyric acid type A (GABA(A)), N-methyl-D-aspartate (NMDA) and acetylcholine receptors. As physiologic consequences, they are involved in neuronal plasticity, learning and memory processes, aggression and epilepsy, and they modulate the responses to stress, anxiety and depression. There is evidence for a common mechanism of action between neurosteroids and sigma1-receptor ligands and focus on the potential therapeutic interests of such interaction in the physiopathology of learning and memory impairments, stress, depression and neuroprotection. [reference 3]

·

Schizophrenia Treatment:

Emerging preclinical and clinical evidence suggests that pregnenolone may be a promising novel therapeutic candidate in schizophrenia. Pregnenolone is a neurosteroid with pleiotropic actions in rodents that include the enhancement of learning and memory, neuritic outgrowth, and myelination. Further, pregnenolone administration results in elevations in downstream neurosteroids such as allopregnanolone, a molecule with neuroprotective effects that also increases neurogenesis, decreases apoptosis and inflammation, modulates the hypothalamic-pituitary-adrenal axis, and markedly increases GABA(A) receptor responses. In addition, pregnenolone administration elevates pregnenolone sulfate, a neurosteroid that positively modulates NMDA receptors. There are thus multiple mechanistic possibilities for pregnenolone as a potential therapeutic agent in schizophrenia, including the amelioration of NMDA receptor hypofunction (via metabolism to pregnenolone sulfate) and the mitigation of GABA dysregulation (via metabolism to allopregnanolone). [reference 6]

·

Results

We ran an HPLC assay for the pregnenelone biobrick to characterise its function. Pregenolone was seen as a clear peak at around 11mins. A standard curve was plotted based on the peaks. The standard curve was then used to calculate the concentration of pregnenolone produced by each sample:

  • Sample with no cholesterol- 0mg/ml
  • Sample with cholesterol in 2-hydroxypropyl beta cyclodextrin- 0.0007mg/ml
  • Sample with cholesterol in DMSO- 0.007mg/ml
Therefore, we got a maximum pregnenolone production of 0.007 mg/ml within 4 hours of substrate addition!