Difference between revisions of "Team:UCL/Protocols"
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<li> Calculate the amount of insert DNA required to maintain 1:3 backbone:insert molar ratio using formula below. For standard ligations use 50 ng of vector DNA, increase the amounts of DNA if unsuccessful.<br/> | <li> Calculate the amount of insert DNA required to maintain 1:3 backbone:insert molar ratio using formula below. For standard ligations use 50 ng of vector DNA, increase the amounts of DNA if unsuccessful.<br/> | ||
<img src="https://static.igem.org/mediawiki/2015/9/9a/UCL_ligationformula.png.png">. </li> | <img src="https://static.igem.org/mediawiki/2015/9/9a/UCL_ligationformula.png.png">. </li> | ||
| − | + | $$Insert Mass in ng = 3*((Insert Length in bp)/(Vector Length in bp))*Vector Mass in ng</math>$$ | |
<li> Prepare the ligation mixture: | <li> Prepare the ligation mixture: | ||
<ul> - required amount of insert DNA</ul> | <ul> - required amount of insert DNA</ul> | ||
Revision as of 22:33, 31 August 2015
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