Difference between revisions of "Team:UCL/Protocols"
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<div id="assays" class="protcl"><h2>Gut on Chip</h2> | <div id="assays" class="protcl"><h2>Gut on Chip</h2> | ||
<h4> | <h4> | ||
+ | <p><b>Fabrication of the upper and lower microchannel</b></p> | ||
<ol> | <ol> | ||
− | + | ||
<li> Pour 3g of 15:1 (wt:wt) PDMS mixture onto the bottom of the petri dish and spread across evenly.</li> | <li> Pour 3g of 15:1 (wt:wt) PDMS mixture onto the bottom of the petri dish and spread across evenly.</li> | ||
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</ol> | </ol> | ||
− | + | <br/> | |
− | <br | + | <p><b>Fabrication of porous membranes</b></p> |
− | + | ||
− | <p><b>Fabrication of porous membranes</b><p> | + | |
<ol> | <ol> | ||
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</ol> | </ol> | ||
+ | <br/> | ||
<p><b>Alignment and Assembly of the micro device</b></p> | <p><b>Alignment and Assembly of the micro device</b></p> | ||
+ | |||
+ | <ol> | ||
<li> Clean the upper PDMS layer and the porous membrane with packaging tape. Do not apply excessive pressure when you are cleaning the membrane surface, in order to avoid unwanted damage of the device and delamination.</li> | <li> Clean the upper PDMS layer and the porous membrane with packaging tape. Do not apply excessive pressure when you are cleaning the membrane surface, in order to avoid unwanted damage of the device and delamination.</li> | ||
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<li>Cut six pieces of 4 – cm long silicone tubing, and connect them to the free ends of the needles </li> | <li>Cut six pieces of 4 – cm long silicone tubing, and connect them to the free ends of the needles </li> | ||
+ | </ol> | ||
</div></div> | </div></div> | ||
</div></div></div> | </div></div></div> |
Latest revision as of 15:10, 17 September 2015
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