Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/17 May 2015"
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= May 17, 2015 = | = May 17, 2015 = | ||
==Growing Cultures== | ==Growing Cultures== | ||
| − | *Prepped 2 tubes of LB(5mL) with chloremphenicol and picked colonies of Bba-xxxx[http://benchling.com/dcancilla/f/mVVgFLnR-honeybee-silk-sequences/seq-cc4djRXz-promsilk-in-psb1c3/edit] to generate a starter culture for protein expression. Also prepped 2 tubes of of LB(5mL) with | + | *Prepped 2 tubes of LB(5mL) with chloremphenicol and picked colonies of Bba-xxxx[http://benchling.com/dcancilla/f/mVVgFLnR-honeybee-silk-sequences/seq-cc4djRXz-promsilk-in-psb1c3/edit] to generate a starter culture for protein expression. Also prepped 2 tubes of of LB(5mL) with kanamycin and picked colonies of PET24a in order to miniprep and generate more plasmid. |
*We are growing the starter culture for the silk protein up to an OD of 0.8. | *We are growing the starter culture for the silk protein up to an OD of 0.8. | ||
**For the starter culture, because we started the incubation at 3pm, and we dont want to overgrow, we will put the cells in the 30 C shaker until tomorrow morning, at which point we will transfer to the 37 degree shaker. | **For the starter culture, because we started the incubation at 3pm, and we dont want to overgrow, we will put the cells in the 30 C shaker until tomorrow morning, at which point we will transfer to the 37 degree shaker. | ||
**The starter culture was placed in the 37 C incubator for one hour, and then transferred to the 30 C incubator at 4 PM | **The starter culture was placed in the 37 C incubator for one hour, and then transferred to the 30 C incubator at 4 PM | ||
Latest revision as of 22:07, 15 July 2015
May 17, 2015
Growing Cultures
- Prepped 2 tubes of LB(5mL) with chloremphenicol and picked colonies of Bba-xxxx[http://benchling.com/dcancilla/f/mVVgFLnR-honeybee-silk-sequences/seq-cc4djRXz-promsilk-in-psb1c3/edit] to generate a starter culture for protein expression. Also prepped 2 tubes of of LB(5mL) with kanamycin and picked colonies of PET24a in order to miniprep and generate more plasmid.
- We are growing the starter culture for the silk protein up to an OD of 0.8.
- For the starter culture, because we started the incubation at 3pm, and we dont want to overgrow, we will put the cells in the 30 C shaker until tomorrow morning, at which point we will transfer to the 37 degree shaker.
- The starter culture was placed in the 37 C incubator for one hour, and then transferred to the 30 C incubator at 4 PM