Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/28 April 2015"
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Latest revision as of 16:35, 5 June 2015
Component | Volume |
5X Q5 Reaction Buffer | 10 |
10 mM dNTPs | 1.0 |
10 uM Forward (primer 3/7) | 2.5 |
10 uM Reverse (primer 8) | 2.5 |
Template (diluted to 1ng/uL) | 1.0 |
Q5 High Fidelity DNA Polymerase | 0.50 |
Nuclease Free Water | 32.5 |
Temperature (C) | Time (Min:sec) |
98 | 0:30 |
98 x 30 cycles | 0:10 |
grad. 62 (silk) 58.4 (silk+prom) x 30 | 0:20 |
72 x 30 | 0:30 |
72(diluted to 1ng/uL) | 2:00 |
10 | hold |