Difference between revisions of "Team:UCLA/Notebook/Honeybee Silk/7 September 2015"

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(→‎Redo of SDS PAGE)
 
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*Ph was 7.6 before adding in the SDS, used ddH20
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**Vinson says SDS screws with the pH meter.

Latest revision as of 22:51, 7 September 2015

iGEM UCLA




Redo of SDS PAGE

  • SDS PAGE to assess different lysis protocols
    • Last time I used the wrong running buffer.
  • Remade the running buffer as follows
    • (1x MOPS running buffer)
Component Mass (g)
Tris Base 6.06
MOPS 10.46
SDS 1.0
EDTA 0.3
  • Ph was 7.6 before adding in the SDS, used ddH20
    • Vinson says SDS screws with the pH meter.