Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/18 August 2015"
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*Used zymo kit to purify BsaI digested M1-AB, BC, CA. | *Used zymo kit to purify BsaI digested M1-AB, BC, CA. | ||
*yields around the 30 ng/uL mark. | *yields around the 30 ng/uL mark. | ||
| + | |||
| + | ==Glycerol Stock Reconstitution== | ||
| + | *Streaked out on individual plates M2-AB, BC, CA | ||
| + | *Streaked old and new glycerol stocks. | ||
Latest revision as of 05:20, 24 August 2015
Contents
8/18/2015
Miniprep and Sequencing for M1-12(1C3)
- Miniprep four samples using Qiagen kit.
| M1-12(1C3) | Concentration (ng/uL) | A 260/280 |
|---|---|---|
| 1 | 261.78 | 1.83 |
| 2 | 295.50 | 1.80 |
| 3 | 311.02 | 1.81 |
| 4 | 272.50 | 1.84 |
- Sent samples for sequencinig.
Gel Purification
- Used zymo kit to purify BsaI digested M1-AB, BC, CA.
- yields around the 30 ng/uL mark.
Glycerol Stock Reconstitution
- Streaked out on individual plates M2-AB, BC, CA
- Streaked old and new glycerol stocks.