Team:UCLA/Notebook/Spider Silk Genetics/27 April 2015

iGEM UCLA




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4/27/2015

4/27/2015


1 2AB, 2BC, 2CA Sticky End Ligation Test


AB+BCBC+CACA+ABAB+BC+CA
2x T7 Ligase Buffer5 uL 5 uL 5 uL 5 uL
ddH2O 1.5 uL 1.5 uL 0.5 uL 0 uL
DNA 1 2 uL 1 uL 2 uL 2 uL
DNA 2 1 uL 2 uL 2 uL 1 uL
DNA 3 n/a n/a n/a 2 uL
T7 Ligase 0.5 uL 0.5 uL 0.5 ul 0.5 uL
Total 10 uL 10 uL 10 uL 10 uL


25 C5 min
65 C

The ligation test was run on 2% gel with 2 uL of 50 bp ladder. Results shown below.


PIC

Figure 1: T7 Ligation Test with MaSp2 fragments. BC was used as a negative ligation control. The presence of ladders may indicate non-specific binding.