Team:UCLA/Parts

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<groupparts>iGEM015 UCLA</groupparts>

PARTS

Honeybee Parts

1. [http://parts.igem.org/Part:BBa_K1763000 BBa_K1763000 ] This part is the coding region of honeybee silk protein #3. For the sequence click here . Click on the link for more details on how the sequence was designed and chosen, as well as how the cloning was carried out.
2. [http://parts.igem.org/Part:BBa_K1763001 BBa_K1763001] This part is a composite part of the honeybee silk protein #3 coding region along with [http://parts.igem.org/Part:BBa_R0010 BBa_R0010 Promoter ] and [http://parts.igem.org/Part:BBa_B0034 RBS BBa_B0034 ]. For the sequence click here . Click HERE for more details on how the sequence was designed and chosen, as well as how the cloning was carried out.
3. [http://parts.igem.org/Part:BBa_K1763008 BBa_K1763008]. This sequence is a fusion protein between our honeybee silk and the Spycatcher, protein which has specific affinity for SpyCatcher. It also contains regulatory elements like a promoter and rbs for expression.
4. [http://parts.igem.org/Part:BBa_K1763007 BBa_K1763007] is our honeybee silk sequence with a T7 promoter upstream for effective protein expression in bacterial strains containing t7 polymerase.
5. [http://parts.igem.org/Part:BBa_K1763015 BBa_K1763015] is our honeybee silk sequence+sfGFP with a T7 promoter and RBS upstream that can be used in bacterial strains that contain T7 polymerase.

Spider Silk Parts

Basic ICA Parts: Monomers

1. [http://parts.igem.org/Part:BBa_K1763002 BBa_K1763002]: Major Ampullate Spidroin 2, construct AB. Used for Iterative Capped Assembly of Spider Silk Genes.
2. [http://parts.igem.org/Part:BBa_K1763003 BBa_K1763003]: Major Ampullate Spidroin 2, construct BC. Used for Iterative Capped Assembly of Spider Silk Genes.
3. [http://parts.igem.org/Part:BBa_K1763003 BBa_K1763004]: Major Ampullate Spidroin 2, construct CA. Used for Iterative Capped Assembly of Spider Silk Genes.
4. [http://parts.igem.org/Part:BBa_K1763009 BBa_K1763009]: Major Ampullate Spidroin 2 Sequencing Core, construct AB. Used in Iterative Capped Assembly of Spider Silk Genes as a unique sequencing primer binding site.

1. [http://parts.igem.org/Part:BBa_K1763010 BBa_K1763010]: Major Ampullate Spidroin 1, construct AB. Used for Iterative Capped Assembly of Spider Silk Genes.
2. [http://parts.igem.org/Part:BBa_K1763011 BBa_K1763011]: Major Ampullate Spidroin 1, construct AB. Used for Iterative Capped Assembly of Spider Silk Genes.
3. [http://parts.igem.org/Part:BBa_K1763012 BBa_K1763012]: Major Ampullate Spidroin 1, construct AB. Used for Iterative Capped Assembly of Spider Silk Genes.
4. [http://parts.igem.org/Part:BBa_K1763423 BBa_K1763423]: Major Ampullate Spidroin 1 Sequencing Core, construct AB. Used in Iterative Capped Assembly of Spider Silk Genes as a unique sequencing primer binding site.

Spider Silk MaSp ICA Constructs

The following constructs were created using Iterative Capped Assembly (ICA) to assemble MaSp monomers.

1. [http://parts.igem.org/Part:BBa_K1763424 BBa_K1763424]: MaSp2-3(1C3)
2. [http://parts.igem.org/Part:BBa_K1763425 BBa_K1763425]: MaSp2-3(T7)
3. [http://parts.igem.org/Part:BBa_K1763426 BBa_K1763426]: MaSp2-6(1C3)
4. [http://parts.igem.org/Part:BBa_K1763427 BBa_K1763427]: MaSp2-6(T7)
5. [http://parts.igem.org/Part:BBa_K1763428 BBa_K1763428]: MaSp2-9(1C3)
6. [http://parts.igem.org/Part:BBa_K1763429 BBa_K1763429]: MaSp2-9(T7)
7. [http://parts.igem.org/Part:BBa_K1763430 BBa_K1763430]: MaSp2-12(1C3)
8. [http://parts.igem.org/Part:BBa_K1763431 BBa_K1763431]: MaSp2-12(T7)
9. [http://parts.igem.org/Part:BBa_K1763432 BBa_K1763432]: MaSp2-15(1C3)
10. [http://parts.igem.org/Part:BBa_K1763433 BBa_K1763433]: MaSp2-15(T7)
11. [http://parts.igem.org/Part:BBa_K1763434 BBa_K1763434]: MaSp1-9(1C3)
12. [http://parts.igem.org/Part:BBa_K1763435 BBa_K1763435]: MaSp1-9(T7)
13. [http://parts.igem.org/Part:BBa_K1763436 BBa_K1763436]: MaSp1-12(1C3)
14. [http://parts.igem.org/Part:BBa_K1763437 BBa_K1763437]: MaSp1-12(T7)
15. [http://parts.igem.org/Part:BBa_K1763438 BBa_K1763438]: MaSp1/2[2:1]-12(1C3)
16. [http://parts.igem.org/Part:BBa_K1763439 BBa_K1763439]: MaSp1/2[2:1]-12(T7)
17. [http://parts.igem.org/Part:BBa_K1763440 BBa_K1763440]: MaSp1/2[1:1]-12(1C3)
18. [http://parts.igem.org/Part:BBa_K1763441 BBa_K1763441]: MaSp1/2[1:1]-12(T7)
19. [http://parts.igem.org/Part:BBa_K1763442 BBa_K1763442]: MaSp1/2[1:2]-12(1C3)
20. [http://parts.igem.org/Part:BBa_K1763443 BBa_K1763443]: MaSp1/2[1:2]-12(T7)

Protein Cage Parts

1. [http://parts.igem.org/Part:BBa_K1763421 BBa_K1763421] Protein Cage Mutant 10