Difference between revisions of "Team:William and Mary/Parts"
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| − | <p><span> | + | <p><span><a href ="http://parts.igem.org/Part:BBa_K1795002">Part:BBa_K17950002</a></span>1</p> |
| − | <p><span> | + | <p><span><a href ="http://parts.igem.org/Part:BBa_K1795003">Part:BBa_K17950003</a></span>2</p> |
| − | <p><span> | + | <p><span><a href ="http://parts.igem.org/Part:BBa_K1795004">Part:BBa_K17950004</a></span>3</p> |
| − | <p><span> | + | <p><span><a href ="http://parts.igem.org/Part:BBa_K1795005">Part:BBa_K17950005</a></span>4</p> |
| + | <p><span><a href ="http://parts.igem.org/Part:BBa_K1795006">Part:BBa_K17950006</a></span>5</p> | ||
| + | <p><span><a href ="http://parts.igem.org/Part:BBa_K1795007">Part:BBa_K17950007</a></span>6</p> | ||
| + | <p><span><a href ="http://parts.igem.org/Part:BBa_K1795008">Part:BBa_K17950008</a></span>7</p> | ||
| + | <p><span><a href ="http://parts.igem.org/Part:BBa_K1795009">Part:BBa_K17950009</a></span>8</p> | ||
| + | <p><span><a href ="http://parts.igem.org/Part:BBa_K1795010">Part:BBa_K17950010</a></span>9</p> | ||
| + | <p><span><a href ="http://parts.igem.org/Part:BBa_K1795011">Part:BBa_K17950011</a></span>10</p> | ||
| + | <p><span><a href ="http://parts.igem.org/Part:BBa_K1795012">Part:BBa_K17950012</a></span>10</p> | ||
<br> | <br> | ||
| − | <p> | + | <p>To complete our creation of a codon-optimized dCas9 parts, we created functional gRNAs that target the most commonly used promoters in iGEM. These parts (BBa_K1795002- BBa_K1795012), when transformed into E. coli, constitutively express gRNA that, in complex with our dCas9 variant, will repress transcription of the targeted promoter. We have also contributed a scramble gRNA (<a href ="http://parts.igem.org/Part:BBa_K17950013">Part:BBa_K17950013</a>) that does not target any region in the E. coli genome and can be used as a negative control. For further details about our gRNAs, please see our <a href = "https://2015.igem.org/Team:William_and_Mary/Part_Collection">Part Collections</a> page. |
| − | + | </p> | |
</div> | </div> | ||
</div> | </div> | ||
Revision as of 13:24, 18 September 2015
All Parts (By Category) In deciding which parts to submit to the iGEM Registry we focused on three main aspects. 1. Ensuring our project is as reproducible and extensible as possible. To that end we have submitted all of new composite fluorescent protein parts that we constructed during the project. 2. Making genome integration as straightforward as possible for iGEM teams. In order to accomplish this goal we designed, tested, and validated a new integrator cassette that allows for simple genome integration using either 3A or Gibson Assembly. 3. Increasing the number of tools available for promoter-mediated regulation in synthetic biology. We created and validated an E. coli codon optimized dCas9 variant and a suite of gRNAs to target the most commonly used promoters in iGEM. Integrator Suites Detail1 Detail2 Detail3 Detail4 text text Antibiotic Operons Detail1 Detail2 Detail3 Detail4 text text dCas9s Part BBa_K1795000 is a dCas9 protein-coding region that has been optimized for expression in E. coli. Additionally, we created a functional dCas9 operon (http://parts.igem.org/Part:BBa_K1795001) that, when transformed into E. coli is constitutively expressed. For further details, please see our Basic Part page. gRNAs To complete our creation of a codon-optimized dCas9 parts, we created functional gRNAs that target the most commonly used promoters in iGEM. These parts (BBa_K1795002- BBa_K1795012), when transformed into E. coli, constitutively express gRNA that, in complex with our dCas9 variant, will repress transcription of the targeted promoter. We have also contributed a scramble gRNA (Part:BBa_K17950013) that does not target any region in the E. coli genome and can be used as a negative control. For further details about our gRNAs, please see our Part Collections page.
XFPs Under Various Promoters Detail1 Detail2 Detail3 Detail4 text text G^2 This plasmid (http://parts.igem.org/Part:BBa_K1795022) consists of both a CFP-LVA driven by R0010 and YFP-LVA driven by R0010. This allows future teams to use this part to investigate transcriptional noise either on a low copy number plasmid or by integrating this part using our galK integrator (http://parts.igem.org/Part:BBa_K1795023).
