1. iGEM distribution kit 2015 was used for this Measurement InterLab study. The following parts were transformed into E.coli dh5alpha strain
BBa_I13504 (kit plate 3, Well 17C)
BBa_K823005, (called K823005 when in pSB1C3) kit Plate 1, Well 20K, pSB1C3
BBa_K823008, (called K823008 when in pSB1C3) kit Plate 1, Well 22A, pSB1C3
BBa_K823013 (called K823013 when in pSB1C3): 2015 Kit Plate 1, Well 22K, pSB1C3
2. Next day we observed colonies on chloramphenicol selective plates. One colony from each plate was put into 5 mL LB + 5 ul chloramphenicol (33 µg/ml). After 8 hours mini- prep was performed by using PureLink Quick Plasmid Miniprep Kit - Life Technologies.
3. Polymerase Chain Reaction was performed on each part using Prefix-F and Suffix-R Primers. After that, pcr products were cleaned up with QIAquick PCR Purification Kit.
PCR- reaction
High Fidelity Master Mix – 10 ul
Prefix-F primer – 2 ul (0.5 ng/ul)
Suffix-R primer – 2 ul (0.5 ng/ul)
DNA (final concentration) - 0.5 ng/ul
Sterile water to 20 ul
Overall volume = 20 ul
