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- <div id="1-1-pcr"> ...25µl reactions were run according to the PCR protocol <a href="#">here</a>500 KB (35,542 words) - 16:53, 18 September 2015
- <div id="1-1-pcr"> ...25µl reactions were run according to the PCR protocol.530 KB (37,526 words) - 03:47, 19 September 2015
- <div id="1-1-pcr"> ...25µl reactions were run according to the PCR protocol <a href="#">here</a>375 KB (27,047 words) - 18:13, 2 September 2015
- need to use high efficiency promoters and lower efficiency RBS. Justin will color:black'>PCR</span></b></p>333 KB (47,812 words) - 07:49, 18 September 2015
- ...h technics, supervised by the instructors. We learned basics, as running a PCR, and where we can find everything needed to work in the lab. Training sessions for PCR56 KB (6,991 words) - 00:06, 19 September 2015
- <div id="1-1-pcr"> ...25µl reactions were run according to the PCR protocol <a href="#">here</a>166 KB (11,675 words) - 10:51, 20 August 2015
- Introduction: Yesterday, Nithin performed a PCR amplication of our PCquad optimized for iGEM 2.0 with the primers to add th PCR amplification of construct attempt #27 KB (1,116 words) - 23:29, 17 July 2015
- allows for quick growth and high yields. Therefore the proper preparation of LB will be crucial to too high – we want to prevent spills.</li>97 KB (16,302 words) - 04:31, 20 November 2015
- <p><strong>Caution:</strong> Gel box has high voltage! Be sure to turn off power pack and unplug leads before removing li Colony PCR Protocols for Transformed <i>E.coli</i>68 KB (11,180 words) - 07:32, 18 September 2015
- <li><a href="#colonypcr">Colony PCR</a></li> ...ropdown-toggle" data-toggle="dropdown" href="#">Polymerase Chain Reaction (PCR)<span class="caret"></span></a>52 KB (3,960 words) - 11:11, 17 August 2015
- <li>PCR for all the single and composite gene parts from 2014 using the protocol sh <li>Q5 high fidelity polymerase 0.25ul</li>36 KB (5,999 words) - 03:43, 19 September 2015
- * gradient PCR using J04450 in pSB1C3 (#ZWDH#) as the template (diluted 1:10) primers are: * run the agarose gel of yesterdays gradient PCR products (#ES4X#) (expected length: 2070 bp)21 KB (3,097 words) - 19:20, 18 September 2015
- <li><a href="#pcr">Polymerase Chain Reaction (PCR)</a> <li><a href="#colonypcr">Colony PCR</a></li>54 KB (5,322 words) - 23:45, 16 September 2015
- <br><a href="#P12">12.PCR Protocols</a> <br><a href="#P13">13.PCR MasterMix</a>30 KB (5,210 words) - 11:44, 18 September 2015
- <li>PCR (Thermo Scientific Phusion High Fidelity PCR Master-mix):<br> <b>Protocol for PCR reaction:</b><br>18 KB (2,876 words) - 00:23, 19 September 2015
- ...ge"><b><u>Goal:</u></b></font> To amplify the Gaussia Luciferase DNA using PCR ...<div class = "indentlist"><ul>Use 5µl in PCR</ul></div>72 KB (10,158 words) - 20:59, 20 November 2015
- <li><p>PCR amplified todE insert and todF insert using FroggaBio 2X Taq FroggaMix</p> <li>Ran PCR purification of TodE and TodF according to the PureLink PCR Purification protocol.</li>21 KB (3,547 words) - 04:32, 20 November 2015
- ...="window.scrollTo(0,document.getElementById('fourth2').offsetTop)">4.2 Taq PCR</li> ...="window.scrollTo(0,document.getElementById('fourth3').offsetTop)">4.3 Pfu PCR</li>107 KB (14,954 words) - 19:51, 18 September 2015
- * Nucleospin Gel and PCR clean-up # Heat in microwave for 1 min at high power.13 KB (2,103 words) - 17:03, 18 September 2015
- ...k() { }); return false;"><h1>PCR product purification using QIAquick® PCR Purification Kit (QIAGEN)</h1></a> ...; Add 5 volumes Buffer PB to 1 volume of the PCR reaction and mix.</p>117 KB (15,375 words) - 00:00, 19 September 2015