Team:BroadRun-NorthernVA/Parts

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PART NAME	PART NUMBER
1. Promoterless alpha amylase with native secretion	BBa_K1871000
2. Alpha amylase with native secretion	BBa_K1871001
3. Alpha amylase with mating factor alpha secretion	BBa_K1871002

Composite Part 1: BBa_K1871000

Promoterless

Kozak sequence (Part BBa_K165002)

Native secretion sequence, from Bacillus amyloliquefaciens

Alpha amylase coding sequence from Bacillus amyloliquefaciens

ADH1 Terminator (Part BBa_K392003)

Composite Part 2: BBa_K1871001

cyc promoter (Part BBa_K105027)

Kozak sequence (Part BBa_K165002)

Native secretion sequence, from Bacillus amyloliquefaciens

Alpha amylase coding sequence from Bacillus amyloliquefaciens

ADH1 Terminator (Part BBa_K392003)

Composite Part 3: BBa_K1871002

cyc promoter (Part BBa_K105027)

Kozak sequence (Part BBa_K165002)

Native secretion sequence, from Bacillus amyloliquefaciens

Alpha amylase coding sequence from Bacillus amyloliquefaciens

ADH1 Terminator (Part BBa_K392003)

Parts Description:

ADH1 Terminator- This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the RNA sequence.

Kozak Sequence- This part initiates translation from eukaryotic mRNA and is cloned between a promoter and coding region to facilitate translation.

cyc minimal promoter- This part is the center of the CYC1 promoter, S. cerevisiae. This promoter allows basal transcription, and the transcription signal can be modulated by adding operator sites upstream of this BioBrick. This allows activators or repressors to act on the transcription machinery.

Mating Factor Alpha Secretion Sequence- This part is the secretion signal from yeast α-mating factor, and directs the secretion of the produced protein. This allows the exportation of the protein.

Alpha Amylase- Obtained from Bacillus amyloliquefaciens, α-Amylase is a protein enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen.

Native Alpha Amylase Secretion Sequence A signal sequence which directs the protein to be secreted.