Running a gel Making the gel Add 100mL of 1x TAE to a flask Add 1g Agarose for a 1% gel Add 2g for a 2% gel, etc. A higher percent gel runs slower but has better resolution Microwave for 60 s Swirl Repeat steps 3 and 4 until agarose is completely dissolved Let cool Add 5ul Ethidium Bromide Preparing the gel Check that open ends of tray are facing the sides of the gel box Alternatively, tape the open ends Put the comb(s) in place Pour If using a larger gel box, pour entire 100mL If using a smaller gel box, pour 75mL or scale down the above amounts Let gel solidify Turn the gel so the comb is at the negative terminal Remove comb Fill gel box with 1X TAE. It should barely cover the gel Running the gel Mix loading dye and water with DNA as needed Vortex and spin briefly Load wells, careful to not let samples out of the well Don't forget the ladder! Plug in and run Smaller gels don't need as high of dosage At a lower voltage, the samples move slower but the separation is more clear
