Team:Colegio EmelinaU/Notebook
Journal of Laboratory
May 30th, 2015
- Genetic transformation of red coloration [B0010]
- Plasmid removing: [K909008]= tetR-DBD-UVR8
[B0010]= Terminator
[R0040]= Promoter
June 13th, 2015
- Genetic transformation of [ B0010 ] * failed . We make a new attempt
- Electrophoresis : [ K909008 ]
[ B0010 ]
[ R0040 ]
June 20th, 2015
- Expanded coloring [ B0010 ]
- Make plasmid removing [ B0010 ]
- Perform digests [ B0010 ] = ( Terminator ) Digestion
[ K909008 ] = ( tetR - DBD - UVR8 ) Double digestion
- electrophoresis : [ B0010 ] 16746p
[ K909008 ] 21506p
June 27th, 2015
- Purification : [ B0010 ]
[ K909008 ]
- Plasmid removing : [ E1010 ]
- Electrophoresis : [ B0010 ]
[ K909008 ]<
[ E1010 ]
July 04th, 2015
- Plasmid removing: [J23119]
[B0010]
[K909008]
[R0040]
[E1010]
- Electrophoresis: [B0010]
[R0040]
[K909008]
[J23119]
- Digestion: [B0010]
[K909008]
July 13th, 2015
- Electrophoresis
- Plasmid Removing
- Terminator digestion and teR-DBD-UVR8
July 14th, 2015
- Bands Purification: [K909008]
[B0010]
- Join [K909008]
[B0010]
- Electroophoresis:
July04th= [K909008];[J23119];[B0010];[R0040]<
[K909008]; [R0040]; [B0010]; [R0040]
- Digestion
July 24th, 2015
Digestion= [K909008]= Xbal-PSTI
[J23119]= SPEI-PSTI
[B0010]= EcoRI-Xbal
[R0040]= SPEI-PSTI
[E1010]= Xbal-PSTI
August 1st, 2015
- Digestion: [B0010]= E X I
[R0040]= S P
[K909008]= X P II
[J23119]= SPEI PSTI
[R0040]= S P I
[R0040]= S P I
[K909008]= X P II N
[B0010]= E X I
- Genetic Transformation: [J23119]= constitutive promoter
[K1084103]= RBS
[K909008]= tetR-DBD-UVR8
[B0010]= Terminator
[R0040]= Promoter
[K592009]= purple color
[K592011]= blue color
August 22nd, 2015
- Digestion: [K1084103]
- Purification: [K909008]
- Genetic transformation: [K592009]
August 29th, 2015
- Plasmid removing: [K592009]
- Purification: [K909008] Black
[K909008] blue
